重组线虫突触相关蛋白25kDa

产品英文名称：
Recombinant Synaptosomal-associated protein 25kDa， C.elegans

英文简称:
SNAP-25

中文名称:
重组线虫突触相关蛋白25kDa

背景资料:
Synaptic vesicle membrane docking and fusion is mediated by SNAREs （soluble N-ethylmaleimide-sensitive factor attachment protein receptors） located on the vesicle membrane （v-SNAREs） and the target membrane （t-SNAREs）. The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices， one of which is supplied by v-SNARE and the other thrRecombinant C.elegans SNAP-25 produced in E.Coli is a single， non-glycosylated polypeptide chain containing 207 amino acids and having a molecular mass of 23kDa.SNAP-25 gene was amplified by PCR from C.elegans and cloned into an E. coli expression vector. SNAP-25 was purified by using conventional chromatography techniques. by t-SNARE. For t-SNAREs on the plasma membrane， the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore， SNAP25 product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. The synaptosomal-associated protein （SNAP-25） is an essential component of the core complex that mediates presynaptic vesicle tra大肠杆菌中重组表达icking. Thus， SNAP-25 is directly involved in the release of neurotransmitters.

产品描述:
Recombinant C.elegans SNAP-25 produced in E.Coli is a single， non-glycosylated polypeptide chain containing 207 amino acids and having a molecular mass of 23kDa.SNAP-25 gene was amplified by PCR from C.elegans and cloned into an E. coli expression vector. SNAP-25 was purified by using conventional chromatography techniques.
来源宿主:
大肠杆菌中重组表达

序列:
MSGDDDIPEG LEAINLKMNA TTDDSLESTR RMLALCEESK EAGIKTLVML DDQGEQLERCEGALDTINQD MKEAEDHLKG MEKCCGLCVL PWNKTDDFEK TEFAKAWKKD DDGGVISDQPRITVGDSSMG PQGGYITKIT NDAREDEMDE NVQQVSTMVG NLRNMAIDMS TEVSNQNRQL DRIHDKAQSN EVRVESANKR AKNLITK.

溶解建议:
Sterile filtered colorless solution. The protein contains 20mM Tris-HCl pH7.5， 50mM NaCl， 5mMDTT， 1mM EDTA and 10% Glycerol.

保存建议：
Store at 4℃ if entire vial will be used within 2-4 weeks.Store， frozen at -20℃ for longer periods of time.For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.Avoid multiple freeze-thaw cycles.
其他简介：

  ProSpec是一家细胞因子蛋白及相关抗体生产和研发品Pai。ProSpec的生产和研发工厂位于以色列，专注于蛋白（重组及合成）生产研发，其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际的科研级蛋白供应商。目前ProSpec可以提供细胞因子，生长因子，激素，信号蛋白，病毒抗原等近800种重组蛋白和100多种抗体，是世界上提供蛋白品种Z多的公司之一，领先的生产工艺和规模使其可以提供毫克到克级蛋白，价格优于同类公司。绝大部分产品是天然成熟型蛋白，而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液，ProSpec-Tany绝大部分产品没有添加保护剂或盐，这就对工艺提出了更高的要求。同时，ProSpec-Tany绝大部分产品为冻干粉，因此易于运输和保存。
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