重组人有丝分裂原激活蛋白激酶1，活性

产品英文名称：
Recombinant Human Mitogen-Activated Protein Kinase 1， Active

英文简称:
MAPK1 Active

中文名称:
重组人有丝分裂原激活蛋白激酶1，活性

背景资料:
Mitogen-activated protein kinase 1 （MAPK1） is also known as "extracellular signal-regulated kinase 2" （ERK2）. Two similar （85% sequence identity） protein kinases were originally called ERK1 and ERK2. They were found during a search for protein kinases that are rapidly phosphorylated after activation of cell surface tyrosine kinasessuch as the epidermal growth factor receptor. Phosphorylation of ERKs leads to the activation of their kinase activity.The molecular events linking cell surface receptors to activation of ERKs are complex. It was found that RasGTP-binding proteins are involved in the activation of ERKs. Another protein kinase， Raf-1， was shown to phosphorylate a "MAPK kinase"， thus qualifying as a "MAPK kinase kinase". The MAPK kinase was named "MAPK/ERK kinase" （MEK）.Receptor-linked tyrosine kinases， Ras， Raf， MEK and MAPK could be fitted into a signaling cascade linking an extracellular signal to MAPK activation.Transgenic gene knockoutmice lacking MAPK1 have major defects in early development.

产品描述:
MAPK1 Recombinant （extracellular signal-regulated kinase） a Mitogen-Activated Protein Kinase， is a highly active form produced by phosphorylation of the purified ERK2/MAPK1 in vitro with MEK1 is a non-glycosylated polypeptide chain containing amino acids 2-480 and having a molecular mass of 44.6 kDa. MAPK1 contains an amino terminal polyhistidine tag and is purified by proprietary chromatographic techniques.
来源宿主:
大肠杆菌中重组表达

序列:


溶解建议:
提供的人有丝分裂原激活蛋白激酶1，活性（MAPK1 Active）产品为过滤灭菌的透明溶液；其中人有丝分裂原激活蛋白激酶1，活性（MAPK1 Active）产品溶解于50mM Tris-HCL，150mM NaCl，1mM DTT，50%甘油，pH 8.5溶液中.

保存建议：
人有丝分裂原激活蛋白激酶1，活性（MAPK1 Active）溶液可以在4℃冷藏保存2-4周；如果想延长保存时间，建议分装后置于-20℃冻存.请避免反复冻融.
其他简介：

  ProSpec是一家细胞因子蛋白及相关抗体生产和研发品Pai。ProSpec的生产和研发工厂位于以色列，专注于蛋白（重组及合成）生产研发，其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际的科研级蛋白供应商。目前ProSpec可以提供细胞因子，生长因子，激素，信号蛋白，病毒抗原等近800种重组蛋白和100多种抗体，是世界上提供蛋白品种Z多的公司之一，领先的生产工艺和规模使其可以提供毫克到克级蛋白，价格优于同类公司。绝大部分产品是天然成熟型蛋白，而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液，ProSpec-Tany绝大部分产品没有添加保护剂或盐，这就对工艺提出了更高的要求。同时，ProSpec-Tany绝大部分产品为冻干粉，因此易于运输和保存。