Pig IL8 / Interleukin 8 ELISA Kit (Sandwich ELISA)Catalog Size Price LS-F23629-1 1 plate $725 USD Available Discounts 5% off 5+ units Request Bulk Add to Wish List Add to Cart SpecificationsPublicationsReviewsImagesProduct DescriptionLS-F23629 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Pig IL8 / Interleukin 8 in samples of Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of IL8 / Interleukin 8 as low as 18.75 picograms per milliliter.SpecificationsType
This kit recognizes natural and recombinant Porcine IL-8. Manual
Intended Sample Types
Plasma, Serum Format
96-Well Microplate Detection
Colorimetric - 450nm (TMB) Measurement
Quantitative Detection Range
31.25 - 2000 pg/ml Sensitivity
18.75 pg/ml Assay Time
3.5 h Precision
Intra-Assay: CV<10% Inter-Assay: CV<10% Storage
Short term: 4°C; Long term: see manual. Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation. Kit Components
User Manual
96-well Microplate
Detection Reagents
Wash Buffer
Substrate
Stop Solution
Adhesive Plate Sealers
Protocol
Prepare all reagents, samples and standards.
Add 100 µl standard or sample to each well and incubate for 90 minutes at 37°C.
Aspirate and add 100 µl prepared Detection Antibody and incubate of 1 hour at 37°C.
Aspirate and wash 3 times.
Add 100 µl HRP Conjugate and incubate for 30 minutes at 37°C.
Aspirate and wash 5 times.
Add 90 µl Substrate Solution and incubate for 15-20 minutes at 37°C.
Add 50 µl Stop Solution.
Read immediately at 450nm.
Restrictions
For research use only. Guarantee
This elisa kit carries the LSBio Guarantee. About IL8 / Interleukin 8
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