The antibody was purified by affinity chromatography、 and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™ .
Concentration
0.5mg/ml
Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining、 the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue?├?├│ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue?├?├│ conjugate for flow cytometric analysis、 please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue?├?├│ is a registered trademark of Molecular Probes、 Inc. Pacific Blue?├?├│ dye antibody conjugates are sold under license from Molecular Probes、 Inc. for research use only、 except for use in combination with microarrays and high content screening、 and are covered by pending and issued patents.
Storage
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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