Clone | 4S.B3 |
Isotype | Mouse IgG1、 κ |
Reactivity | Human、 Cross-Reactivity: Chimpanzee、 Baboon、 Cynomolgus、 Rhesus |
Immunogen | Partially purified、 native human IFN-γ |
Formulation | Phosphate-buffered solution、 pH 7.2、 containing 0.09% sodium azide and BSA (origin USA). |
Preparation | The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 650™ and unconjugated antibody. |
Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining、 the suggested use of this reagent is ≤5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection、 although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd. |
Storage | The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |