Recombinant Naja kaouthia Alpha-cobratoxin

host_species:E.coli

purification:Greater than 85% as determined by SDS-PAGE.

other_names:Alpha-elapitoxin-Nk2aShort name:Alpha-EPTX-Nk2aLong neurotoxin 1Siamensis 3

accession_no:P01391

calculated_mw:37.8 kDa

tag info:N-terminal 10xHis-GST-tagged and C-terminal Myc-tagged

Immunogen Description:Expression Region:1-71aa
Sequence Info:Full Length

formulation:Tris-based buffer50% glycerol

storage:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃,-80℃. The shelf life of lyophilized form is 12 months at -20℃,-80℃.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

background:Monomer: binds with high affinity to muscular (alpha-1-beta-1-gamma-delta (CHRNA1,CHRNB1,CHRNG,CHRND) nAChR) (IC50=4.5 nM on Torpedo californica membranes) and neuronal alpha-7,CHRNA7 nicotinic acetylcholine receptors (IC50=105 nM).2 PublicationsHomodimer: binds with high affinity (but lower than the monomeric form) to muscular (IC50=9.7 nM) and with low affinity to neuronal alpha-7,CHRNA7 nAChRs (IC50=1370 nM).However, it acquires (compared to the monomeric form) the capacity to block alpha-3,beta-2 (CHRNA3,CHRNB2) nAChRs Heterodimer with cytotoxin 3 (AC P01446): is slightly more active than the homodimer in inhibiting alpha-7 nAChR and is considerably more active in blocking the alpha-3-beta-2 nAChR.The monomeric form has no effect on alpha-3,beta-2 (CHRNA3,CHRNB2) nAChR. It does not show any blockade of the nicotine-evoked release of dopamine and does not affect ACh release.

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