μMACS SuperAmp Starting Kit/µMACS SuperAmp Kits

品Pai:miltenyi，产品名称：µMACS SuperAmp Starting Kit
µMACS SuperAmp Kits - Miltenyi Biotec
货号：130-093-251
The µMACS SuperAmp Kit facilitates fast and effective mRNA isolation, cDNA synthesis, and a millionfold amplification of the mRNA-derived cDNA by global PCR starting with 1 to 10,000 cells or appropriate amounts of tissue, without prior preparation of total RNA. Pure mRNA is directly isolated from cells, tissue, or blood by magnetic labeling with µMACS Oligo(dT) MicroBeads. Due to the fast reaction kinetics the small (50 nm) µMACS Oligo(dT) MicroBeads instantly bind to mRNA molecules and allow for efficient and reproducible isolation of high-purity mRNA. Subsequent cDNA synthesis, and cDNA purification take place in the same column, thus reducing loss of material and increasing the sensitivity of analysis. The synthesis of uniform sized cDNA fragments and their 3’ and 5’ tailing allows for a homogenous and uniform amplification of the cDNA during single-primer PCR, thus reducing PCR bias.