Streptavidin (Sepharose Bead)

品Pai:CST，产品名称：Streptavidin (Sepharose Bead Conjugate) #3419
英文名称：Streptavidin (Sepharose® Bead Conjugate)
Product name：Streptavidin (Sepharose^® Bead Conjugate) #3419
Isotype：Mouse IgG2a
Sensitivity：Transfected Only
Applications：IP ,
Background： Streptavidin is a 53,000 dalton tetrameric protein purified from the bacterium Streptomyces avidinii (3). Each subunit binds to biotin with extremely high affinity. Because of its strong non-covalent interaction with biotin, streptavidin can be used to isolate biotinylated proteins (1,2).

Add 10 μl of well-vortexed beads to 200 μl of cell lysate at 1 mg/ml pre-incubated with biotinylated primary antibody. See protocol for more details.

Storage:

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

Product Description:

Streptavidin (Sepharose^® Bead Conjugate) is useful for the precipitation of biotinylated proteins (1,2). Recombinant streptavidin is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated Sepharose^® beads.