Apoptosis (Cleaved Caspase-3) IHC Detection Kit

品Pai:CST，产品名称：SignalStain Apoptosis (Cleaved Caspase-3) IHC Detection Kit #12692
英文名称：SignalStain® Apoptosis (Cleaved Caspase-3) IHC Detection Kit
Product name：SignalStain^® Apoptosis (Cleaved Caspase-3) IHC Detection Kit #12692
Applications：IHC ,
Purification： Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp175 of human caspase-3 protein. Rabbit (DA1E) mAb IgG XP^® SignalStain^® Isotype Control is not directed against any known antigen. It functions as an isotype control for rabbit IgG monoclonal antibodies.
Background： Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2).

Entrez-Gene Id

Swiss-Prot Acc.

Specificity / Sensitivity:

SignalStain^® Apoptosis (Cleaved Caspase-3) IHC Detection Kit detects endogenous levels of the activated caspase-3 large fragment (17/19 kDa) resulting from cleavage adjacent to Asp175. This antibody does not recognize full-length caspase-3 or other cleaved caspases. This kit was developed for and is recommended for immunohistochemistry only.

Species Reactivity:

Human, Mouse

Product Description:

SignalStain^® Apoptosis (Cleaved Caspase-3) IHC Detection Kit allows the detection of activated caspase-3 in formalin-fixed paraffin-embedded human and mouse tissue samples. Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb is detected by the polymer based, HRP-conjugated SignalStain^® Boost IHC Detection Reagent in combination with SignalStain^® DAB Diluent and Chromogen Concentrate. Also included is a concentration-matched rabbit monoclonal IgG control to verify the specificity of staining.

This combination of reagents provides a sensitive and specific means of detecting apoptotic events in tissue samples.