EDTA Unmasking Solution (10X)

品Pai:CST，产品名称：SignalStain EDTA Unmasking Solution (10X) #14747
英文名称：SignalStain® EDTA Unmasking Solution (10X)
Product name：SignalStain^® EDTA Unmasking Solution (10X) #14747
Applications：IHC ,
Background： Proteins within fixed tissue sections contain chemical cross-links that may prevent antibody access or mask antigen targets in immunohistochemical assays. This limited access may vary among different tissue or antigens. Heat-induced antigen unmasking improves accessibility of antibodies to tissues or antigens (1).

Dilute to a 1X Unmasking Solution using ddH₂O just before use. Bring slides to a boil in 1X SignalStain^® EDTA Unmasking Solution. Follow with 15 min at a sub-boiling temperature. No cooling is necessary.

Antigen unmasking protocols utilizing a water bath are not recommended. While antigen unmasking performed with a microwave is preferred, the staining of particular tissues or antigen targets may require the use of a pressure cooker.

Storage:

Store 10X buffer at 4°C. At 10X, this buffer is stable for 12 months. Always prepare fresh 1X solutions daily.

Product Description:

SignalStain^® EDTA Unmasking Solution (10X) is used for antigen unmasking of formalin fixed, paraffin-embedded tissue sections or cell pellets in immunohistochemical assays (IHC-P). Cell Signaling Technology recommends the optimal unmasking reagent for each IHC-P approved antibody. Please consult the primary antibody datasheet to determine if this solution is recommended for your specific product.