Phospho-β-Catenin (Ser45) Antibody

品Pai:CST，产品名称：Phospho-β-Catenin (Ser45) Antibody #9564
英文名称：Phospho-β-Catenin (Ser45) Antibody
Product name：Phospho-β-Catenin (Ser45) Antibody #9564
Source：Rabbit
Sensitivity：Endogenous
MW(kDa)：92
Reactivity：H
Applications：WB ,
Purification： Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser45 of human β-catenin. Antibodies are purified by protein A and peptide affinity chromatography.
Background： β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).

Entrez-Gene Id

Swiss-Prot Acc.

Specificity / Sensitivity:

Phospho-β-Catenin (Ser45) Antibody detects endogenous levels of beta-catenin only when phosphorylated at serine 45. It does not recognize β-catenin phosphorylated at other sites.

Species Reactivity:

Human

Species predicted to react based on sequence homology:

Mouse, Rat, Dog, Pig

Storage:

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.