Phospho-Stat Antibody Sampler Kit

品Pai:CST，产品名称：Phospho-Stat Antibody Sampler Kit #9914
英文名称：Phospho-Stat Antibody Sampler Kit
Product name：Phospho-Stat Antibody Sampler Kit #9914
Isotype：Rabbit IgG
Sensitivity：Endogenous
MW(kDa)：84, 91
Purification： Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptides corresponding to residues surrounding Tyr690 of human Stat2, Ser727 of mouse Stat3, or Tyr641 of human Stat6. Antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr701 of human Stat1 protein, Tyr705 of mouse Stat3, or residues surrounding Tyr694 of human Stat5a protein.
Background： Jaks (Janus Kinases) and Stats (Signal Transducers and Activators of Transcription) are utilized by receptors for a wide variety of ligands including cytokines, hormones, growth factors and neurotransmitters. Jaks, activated via autophosphorylation following ligand-induced receptor aggregation, phosphorylate tyrosine residues on associated receptors, Stat molecules and other downstream signaling proteins (1,2). The phosphorylation of Stat proteins at conserved tyrosine residues activates SH2-mediated dimerization followed rapidly by nuclear translocation. Stat dimers bind to IRE (interferon response element) and GAS (gamma interferon-activated sequence) DNA elements, resulting in the transcriptional regulation of downstream genes (1,2). The remarkable range and specificity of responses regulated by the Stats is determined in part by the tissue-specific expression of different cytokine receptors, Jaks and Stats (2,3), and by the combinatorial coupling of various Stat members to different receptors. Serine phosphorylation in the carboxy-terminal transcriptional activation domain has been shown to regulate the function of Stat1, -2, -3, -4 and -5 (1). Phosphorylation of Stat3 at Ser727 via MAPK or mTOR pathways is required for optimal transcriptional activation in response to growth factors and cytokines including IFN-gamma and CNTF (4,5). Jak/Stat pathways also play important roles in oncogenesis, tumor progression, angiogenesis, cell motility, immune responses and stem cell differentiation (6-11).

Entrez-Gene Id

6772, 6773, 6774, 6776, 6777, 6778

Swiss-Prot Acc.

P42224, P52630, P40763, P42229, P51692, P42226

Specificity / Sensitivity:

Each phospho-Stat antibody in the kit recognizes only the phosphorylated form of its specific target.

Protocols

• 4322:Western Blotting, Immunofluorescence*, Flow
• 4441:Western Blotting
• 7074:Western Blotting
• 7649:Western Blotting, Immunoprecipitation (Magnetic), Immunofluorescence*, Flow, ChIP Magnetic, Chromatin IP-seq
• 9134:Western Blotting, Immunoprecipitation (Magnetic), ChIP Magnetic
• 9145:Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunofluorescence*, Flow, ChIP Magnetic, Chromatin IP-seq
• 9361:Western Blotting, Immunoprecipitation (Magnetic), Immunofluorescence*, Flow

Product Includes	Quantity	Applications	Reactivity	MW(kDa)	Isotype
Phospho-Stat1 (Tyr701) (D4A7) Rabbit mAb 7649	20 µl	WB IP IF F ChIP	H M R	84, 91	Rabbit IgG
Phospho-Stat2 (Tyr690) Antibody 4441	20 µl	WB	H	113	Rabbit
Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb 9145	20 µl	WB IP IHC IF F ChIP	H M R Mk	79, 86	Rabbit IgG
Phospho-Stat3 (Ser727) Antibody 9134	20 µl	WB IP ChIP	H M R	86	Rabbit
Phospho-Stat5 (Tyr694) (D47E7) XP® Rabbit mAb 4322	20 µl	WB IF F	H M	90	Rabbit IgG
Phospho-Stat6 (Tyr641) Antibody 9361	20 µl	WB IP IF F	H	110	Rabbit
Anti-rabbit IgG, HRP-linked Antibody 7074	100 µl	WB	Goat

Product Description:

The Phospho-Stat Pathway Sampler Kit provides an economical means to evaluate the activation status of Stat molecules, including the phosphorylation of Stat1 at Tyr701, Stat2 at Tyr690, Stat3 at Tyr705/Ser727, Stat5 at Tyr694 and Stat6 at Tyr641. The kit includes enough primary and secondary antibody to perform two Western blot experiments.