Phospho-MFF (Ser146) Antibody

品Pai:CST，产品名称：Phospho-MFF (Ser146) Antibody #49281
英文名称：Phospho-MFF (Ser146) Antibody
Product name：Phospho-MFF (Ser146) Antibody #49281
Source：Rabbit
Sensitivity：Endogenous
MW(kDa)：25, 27, 30, 35
Applications：WB ,
Purification： Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser146 of human MFF isoform 2 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background： Mitochondrial fission factor (MFF) is a tail-anchored protein that resides within the outer mitochondrial membrane and is part of the mitochondrial fission complex. MFF participates in mitochondrial fission by serving as one of multiple receptors for the GTPase dynamin-related protein 1 (Drp1) (1-4). Research studies have also shown that MFF is a peroxisomal membrane protein and participates in peroxisome fission by serving as a receptor for another GTPase, dynamin-like protein 1 (5,6).Research studies have demonstrated that the ability of MFF to drive acute mitochondrial fission in response to mitochondrial stress is controlled by AMPK-dependent phosphorylation. AMPK directly phosphorylates MFF at two sites to allow for enhanced recruitment of Drp1 to the mitochondra (7). Multiple isoforms of MFF are expressed as a result of alternative splicing (8). One of the major phosphoacceptor sites of MFF (Ser172 of human isoform 1/Ser146 of human isoforms 2-5) lies within an AMPK phsophorylation motif that spans the boundary of differentially spliced exons of MFF isoforms, suggesting that MFF splice isoforms are phosphorylated by AMPK to varying degrees.

Entrez-Gene Id

Swiss-Prot Acc.

Specificity / Sensitivity:

Phospho-MFF (Ser146) Antibody recognizes endogenous levels of MFF protein only when phosphorylated at Ser146. This antibody has been shown to react with phosphorylated Ser146 of human MFF isoforms 2, 4, and 5 but not phosphorylated Ser172 of human MFF isoform 1 (7). This antibody cross-reacts with a 140kDa protein of unknown identity.

Species Reactivity:

Human, Mouse, Rat

Storage:

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.