Anti-POMC(Alpha-MSH) antibody

Anti-POMC(Alpha-MSH) antibody

Cat. No.     JLC260635        
Package    25 μl/100 μl/200 μl
Storage    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Product overview
Description                   Anti-POMC(Alpha-MSH) rabbit polyclonal antibody
Applications        ELISA, WB 
Immunogen               Synthetic peptide of human POMC
Reactivity                 Human, Mouse, Rat
Content                     0.2 mg/ml
Host species               Rabbit
Ig class                  Immunogen-specific rabbit IgG
Purification                 Antigen affinity purification

Target information
Symbol                 POMC
Full name                 proopiomelanocortin
Synonyms    LPH, MSH, NPP, POC, ACTH, CLIP
Swissprot    P01189

Target Background
This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation.

Applications
Western blotting
Predicted band size:29 kDa
Positive control:Human fetal brain tissue 
Recommended dilution: 500-2000

Gel: 10%SDS-PAGE
Lysate: 40 μg
Lane: Human fetal brain tissue
Primary antibody: ml260635(POMC(Alpha-MSH) Antibody) at dilution 1/400
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 10 seconds
                        
ELISA
Recommended dilution:2000-5000