英文名称 | Anti-His tag(CT)/FITC |
中文名称 | FITC标记的聚组氨酸标签抗体(C端) |
别 名 | HisX6; 6His; His Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag. |
规格价格 | 100ul/2980元 购买 大包装/询价 |
说 明 书 | 100ul |
研究领域 | 染色质和核信号 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | fusion protein, HHHHHH tagged |
产品应用 | IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | Synthetic peptide: HHHHHH conjugated to KLH |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
产品介绍 | background: The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 标签抗体 蛋白质纯化的新途径─应用His-Tag抗体检测His-Tag融合蛋白 1) 6xHis与不同的Sepharose纯化填料结合,可不受构象的影响,在天然或变形的条件下可进行进一步纯化。 2) 可在温和的洗脱液环境中,其结合、洗涤及洗脱步骤不会对蛋白结构产生影响。 3) 6xHis标签比普通(Tag)要小得多,经纯化后的蛋白可直接进行下游操作。可用于任何表达系统(包括: 细菌,杆状病毒和哺乳动物) 4) 6xHis标签在生理溶液pH下不带电荷,其标签不会干涉重组蛋白的结构和功能, 6xHis不会影响蛋白分泌 5) 6xHis标签免疫原性差,重组蛋白可以不必除去标签而直接作为抗原进行免疫。 |