Mouse FGF21 ELISA Kit (Sandwich ELISA) - LS-F35298

Mouse FGF21 ELISA Kit (Sandwich ELISA) - LS-F35298
Catalog
Size
Price
LS-F35298-1
1 plate
$990 USD
Request Bulk
Add to Wish List
Add to CartPerformance Guaranteed



SpecificationsPublicationsReviewsImagesPopular FGF21 Elisa Kits
Human FGF21 ELISA Kit (Sandwich ELISA) - LS-F23399
Reactivity: Human
Range: 31.25-2000 pg/ml

Mouse FGF21 ELISA Kit (Sandwich ELISA) - LS-F23493
Reactivity: Mouse
Range: 31.25-2000 pg/ml

Pig FGF21 ELISA Kit (Sandwich ELISA) - LS-F23643
Reactivity: Pig
Range: 31.25-2000 pg/ml

Pig FGF21 ELISA Kit (Sandwich ELISA) - LS-F7600
Reactivity: Pig
Range: 15.63-1000 pg/ml

Human FGF21 ELISA Kit (Sandwich ELISA) - LS-F2817
Reactivity: Human
Range: 31.2-2000 pg/ml

---

Product DescriptionLS-F35298 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse FGF21 in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of FGF21 as low as 0.938 picograms per milliliter.
More FGF21 Products
Top of Page

---

SpecificationsType
 
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
 
FGF21
Synonyms
 
FGF21, Fibroblast growth factor 21, FGF-21
Reactivity
 
Mouse
Manual
 

Intended Sample Types
 
Plasma, Serum, Tissue Homogenates
Format
 
96-Well Strip Plate
Detection
 
Colorimetric - 450nm (TMB)
Measurement
 
Quantitative
Detection Range
 
1.563 - 100 pg/ml
Sensitivity
 
0.938 pg/ml
Precision
 
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
 
Store at 4°C. Stable for 6 months.
Quality Assurance
 
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
 

• User Manual
• 96-well Microplate
• Detection Reagents
• Wash Buffer
• Substrate
• Stop Solution
• Adhesive Plate Sealers

Background
 
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Fgf21 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Fgf21 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Fgf21 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Fgf21 can be calculated.
Restrictions
 
For research use only.
Guarantee
 
This elisa kit carries the LSBio Guarantee.
About FGF21
 
Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. Uniprot: Q9NSA1 NCBI:NM_019113 NP_061986.1

 

Top of Page

---

Publications (0)Submit a Publication
Top of Page

---

Customer Reviews (0)Submit a Review
Top of Page

---

Images

 

 

Sandwich ELISA Platform Overview