Pig F2 / Prothrombin / Thrombin ELISA Kit (Competitive EIA) - LS-F51072

Pig F2 / Prothrombin / Thrombin ELISA Kit (Competitive EIA) - LS-F51072
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Size
Price
LS-F51072-1
1 plate
$1,090 USD
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10% off 5+ units
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SpecificationsPublicationsReviewsImagesPopular F2 / Prothrombin / Thrombin Elisa Kits
Bovine F2 / Prothrombin / Thrombin ELISA Kit (Sandwich ELISA) - LS-F13468
Reactivity: Bovine
Range: 7.8-500 ng/ml

Human F2 / Prothrombin / Thrombin ELISA Kit (Sandwich ELISA) - LS-F13466
Reactivity: Human
Range: 0.78-50 ng/ml

Human F2 / Prothrombin / Thrombin ELISA Kit (Sandwich ELISA) - LS-F22981
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Human F2 / Prothrombin / Thrombin ELISA Kit (Sandwich ELISA) - LS-F4268
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Rat F2 / Prothrombin / Thrombin ELISA Kit (Sandwich ELISA) - LS-F13467
Reactivity: Rat
Range: 1.56-100 ng/ml

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Product DescriptionLS-F51072 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Pig F2 / Prothrombin / Thrombin in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Competitive EIA assay principle and can be used to detect levels of F2 / Prothrombin / Thrombin as low as 3.9 nanograms per millilter.
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SpecificationsType
 
Competitive EIA (enzyme immunoassay) kit
Target
 
F2 / Prothrombin / Thrombin
Synonyms
 
F2, Prothrombin B-chain, Coagulation factor II, FACTOR II, Prothrombin, PT, RPRGL2, Serine protease, THPH1, Thrombin
Reactivity
 
Pig
Specificity
 
This assay has high sensitivity and excellent specificity for detection of pig FII. No significant cross-reactivity or interference between pig FII and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between pig FII and all the analogues, therefore, cross reaction may still exist.
Manual
 

Intended Sample Types
 
Plasma, Serum, Tissue Homogenates
Sample Size
 
50 - 100 µl
Format
 
96-Well Strip Plate
Detection
 
Colorimetric - 450nm (TMB)
Measurement
 
Quantitative
Detection Range
 
3.9 - 1000 ng/ml
Sensitivity
 
3.9 ng/ml
Assay Time
 
2.5 h
Precision
 
Intra-Assay: CV<8% / Inter-Assay: CV<10%
Storage
 
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Quality Assurance
 
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
 

• User Manual
• 96-well Microplate
• Detection Reagents
• Wash Buffer
• Substrate
• Stop Solution
• Adhesive Plate Sealers

Restrictions
 
For research use only.
Guarantee
 
This elisa kit carries the LSBio Guarantee.
About F2 / Prothrombin / Thrombin
 
Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Finally, peptides derived from the C-terminus of this protein have antimicrobial activity against E. coli and P. aeruginosa. Mutations in F2 leads to various forms of thrombosis and dysprothrombinemia. Uniprot: P00734 NCBI: NM_000506 NP_000497.1

 

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