Human Estradiol ELISA Kit (Competitive EIA) - LS-F10021

Human Estradiol ELISA Kit (Competitive EIA) - LS-F10021
Catalog
Size
Price
LS-F10021-1
1 plate
$640 USD
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10% off 5+ units
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Product DescriptionLS-F10021 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human Estradiol in samples of Cell Culture Supernatants, Plasma, Serum, Tissue Homogenates and Urine. It is based upon a Competitive EIA assay principle and can be used to detect levels of Estradiol as low as 40 picograms per milliliter.
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SpecificationsType
 
Competitive EIA (enzyme immunoassay) kit
Target
 
Estradiol
Reactivity
 
Human
Specificity
 
This assay has high sensitivity and excellent specificity for detection of human E2. No significant cross-reactivity or interference between human E2 and analogs was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human E2 and all the analogs, therefore, cross reaction may still exist.
Manual
 

Intended Sample Types
 
Cell Culture Supernatants, Plasma, Serum, Tissue Homogenates, Urine
Sample Size
 
50 - 100 µl
Format
 
96-Well Microplate
Detection
 
Colorimetric - 450nm (TMB)
Measurement
 
Quantitative
Detection Range
 
40 - 1500 pg/ml
Sensitivity
 
40 pg/ml
Precision
 
Intra-assay: CV%<15% Inter-assay: CV%<15%
Storage
 
Short term: 4°C; Long term: see manual.
Quality Assurance
 
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
 

• User Manual
• 96-well Microplate
• Detection Reagents
• Wash Buffer
• Substrate
• Stop Solution
• Adhesive Plate Sealers

Background
 
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for E2 and Horseradish Peroxidase (HRP) conjugated E2. The competitive inhibition reaction is launched between with HRP labeled E2 and unlabeled E2 with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of E2 in the sample. The color development is stopped and the intensity of the color is measured.
Restrictions
 
For research use only.
Guarantee
 
This elisa kit carries the LSBio Guarantee.
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