Rat ER Alpha / Estrogen Receptor ELISA Kit (Sandwich ELISA) - LS-F53765

Rat ER Alpha / Estrogen Receptor ELISA Kit (Sandwich ELISA) - LS-F53765
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Size
Price
LS-F53765-1
1 plate
$1,315 USD
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10% off 5+ units
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SpecificationsPublicationsReviewsImagesPopular ER Alpha / Estrogen Receptor Elisa Kits
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Product DescriptionLS-F53765 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat ER Alpha / Estrogen Receptor in samples of Cell Lysates, Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of ER Alpha / Estrogen Receptor as low as 7.81 picograms per milliliter.
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SpecificationsType
 
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
 
ER Alpha / Estrogen Receptor
Synonyms
 
ESR1, Era, ERalpha, Estrogen receptor, Estradiol receptor, ER-alpha, Estrogen receptor 1, NR3A1, ER, ESR, ESRA, Estrogen receptor alpha
Reactivity
 
Rat
Specificity
 
This assay has high sensitivity and excellent specificity for detection of rat ER. No significant cross-reactivity or interference between rat ER and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between rat ER and all the analogues, therefore, cross reaction may still exist.
Manual
 

Intended Sample Types
 
Cell Lysates, Plasma, Serum, Tissue Homogenates
Sample Size
 
50 - 100 µl
Format
 
96-Well Strip Plate
Detection
 
Colorimetric - 450nm (TMB)
Measurement
 
Quantitative
Detection Range
 
31.25 - 2000 pg/ml
Sensitivity
 
7.81 pg/ml
Assay Time
 
4.5 h
Precision
 
Intra-Assay: CV<8% / Inter-Assay: CV<10%
Storage
 
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Quality Assurance
 
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
 

• User Manual
• 96-well Microplate
• Detection Reagents
• Wash Buffer
• Substrate
• Stop Solution
• Adhesive Plate Sealers

Restrictions
 
For research use only.
Guarantee
 
This elisa kit carries the LSBio Guarantee.
About ER Alpha / Estrogen Receptor
 
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Uniprot: P03372 NCBI: NM_000125 NP_000116.2

 

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