Rat EDN1 / Endothelin 1 ELISA Kit (Sandwich ELISA) - LS-F24935 Reactivity: Rat Range: 3.9-250 pg/mlProduct DescriptionLS-F53583 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rabbit EDN1 / Endothelin 1 in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of EDN1 / Endothelin 1 as low as 1.56 picograms per milliliter. More EDN1 / Endothelin 1 Products Top of PageSpecificationsType
This assay has high sensitivity and excellent specificity for detection of rabbit ET-1. No significant cross-reactivity or interference between rabbit ET-1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between rabbit ET-1 and all the analogues, therefore, cross reaction may still exist. Manual
Intended Sample Types
Plasma, Serum, Tissue Homogenates Sample Size
50 - 100 µl Format
96-Well Strip Plate Detection
Colorimetric - 450nm (TMB) Measurement
Quantitative Detection Range
6.25 - 400 pg/ml Sensitivity
1.56 pg/ml Assay Time
4.5 h Precision
Intra-Assay: CV<8% / Inter-Assay: CV<10% Storage
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles. Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation. Kit Components
Coated 96-well Strip Plate
Adhesive Plate Sealers
Sample Diluent
Wash Buffer (25x)
TMB Substrate
Stop Solution
Standard (Lyophilized)
HRP Conjugate Diluent
Biotinylated Detection Antibody Diluent
HRP Conjugate (100x)
Biotinylated Detection Antibody (100x)
Restrictions
For research use only. Guarantee
This elisa kit carries the LSBio Guarantee. About EDN1 / Endothelin 1
EDN1 / Endothelin 1 is proteolytically processed to release a secreted peptide termed endothelin 1. This peptide is a potent vasoconstrictor and is produced by vascular endothelial cells. Endothelin 1 also can affect the central nervous system. Two transcript variants encoding different isoforms have been found for this gene.P05305 NM_001955 NP_001946.3
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