货号:
BH-K22613
保存条件:
Store at -20 °C
浓度:
1mg/1ml
应用范围:
WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
形态:
详见说明书
亚型:
IgG
免疫原:
KLH conjugated synthetic peptide derived from human SNAP25BP
适应物种:
详见说明书
宿主:
详见说明书
标记物:
详见说明书
抗体名:
突触相关蛋白25结合蛋白抗体规格
抗体英文名:
Anti-SNAPIN/SNAP25BP
保质期:
详见说明书
抗原来源:
Rabbit
克隆性:
多克隆
目录编号:
详见说明书
级别:
详见说明书
靶点:
详见说明书
数量:
大量
供应商:
上海博湖
规格:
0.2ml/200μg
英文名称 Anti-SNAPIN/SNAP25BP
中文名称 突触相关蛋白25结合蛋白抗体规格别 名 SNAP associated protein; SNAP-25-binding protein; SNAP-associated protein; SNAP25BP; SNAPAP; SNAPIN; SNAPN_HUMAN; SNARE associated protein snapin; SNARE-associated protein Snapin; Synaptosomal associated protein 25 binding protein; Synaptosomal-associated protein 25-binding protein; OTTHUMP00000035157.
突触相关蛋白25结合蛋白抗体规格浓 度 1mg/1ml
规 格 0.1ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit
产品类型 一抗
研究领域 细胞生物 神经生物学 信号转导 细胞类型标志物
蛋白分子量 predicted molecular weight: 15kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human SNAP25BP
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4突触相关蛋白25结合蛋白抗体规格产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 The protein encoded by this gene is a coiled-coil-forming protein that associates with the SNARE (soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor) complex of proteins and the BLOC-1 (biogenesis of lysosome-related organelles) complex. Biochemical studies have identified additional binding partners. As part of the SNARE complex, it is required for vesicle docking and fusion and regulates neurotransmitter release. The BLOC-1 complex is required for the biogenesis of specialized organelles such as melanosomes and platelet dense granules. Mutations in gene products that form the BLOC-1 complex have been identified in mouse strains that are models of Hermansky-Pudlak syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012].Function : May modulate a step between vesicle priming, fusion and calcium-dependent neurotransmitter release by potentiating the interaction of synaptotagmins with the SNAREs and the plasma-membrane-associated protein SNAP25. Its phosphorylation state influences exocytotic protein interactions and may regulate synaptic vesicle exocytosis. May also have a role in the mechanisms of SNARE-mediated membrane fusion in non-neuronal cells.Subunit : Interacts with CSNK1D, SNAP23 and STX4A but not with STX1A, VAMP2 and SYT1. Interacts with SNAP25; the interaction with SNAP25 is increased by its phosphorylation (By similarity). Component of the biogenesis of lysosome-related organelles complex 1 (BLOC-1) composed of BLOC1S1, BLOC1S2, BLOC1S3, BLOC1S4, BLOC1S5, BLOC1S6, DTNBP1/BLOC1S7 and SNAPIN/BLOC1S8. Octamer composed of one copy each BLOC1S1, BLOC1S2, BLOC1S3, BLOC1S4, BLOC1S5, BLOC1S6, DTNBP1/BLOC1S7 and SNAPIN/BLOC1S8. The BLOC-1 complex associates with the AP-3 protein complex and membrane protein cargos. Associates with the SNARE complex. Interacts with CEP110, NANOS1, PUM2 and RGS7. Interacts with human cytomegalovirus/HHV-5 protein UL70.Subcellular Location : Cytoplasm (By similarity). Membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Peripheral membrane protein; Cytoplasmic side (Potential). Cell junction, synapse, synaptosome. Cytoplasm, perinuclear region. Golgi apparatus membrane (By similarity). Note=May be cytoplasmic and peripheral membrane bound or anchored to the vesicular membrane through an N-terminal signal anchor (By similarity). Co-localizes with NANOS1 and PUM2 in the perinuclear region of germ cells.Tissue Specificity : Expressed in male germ cells of adult testis (at protein level).Post-translational modifications : Phosphorylated by CSNK1D/CK1 (By similarity).Similarity : Belongs to the SNAPIN family.Database links : UniProtKB/Swiss-Prot: O95295.1突触相关蛋白25结合蛋白抗体规格抗体的鉴定:
1)抗体的效价鉴定:不管是用于诊断还是用于ZL,制备抗体的目的都是要求较GX价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。
2)抗体的特异性鉴定:抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争YZ试验测定。以不同浓度抗原和近似抗原分别做竞争YZ曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。
如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。
3)亲和力:是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。
种属:
兔抗单克隆抗体,鼠抗单克隆抗体。兔抗多克隆抗体。抗体的浓度为1mg/ml。 抗体及相关标记抗体:HRP标记抗体,Biotin标记抗体,Gold标记抗体,RBITC标记抗体,AP标记抗体,FITC标记抗体,Cy3标记抗体,Cy5标记抗体,Cy5.5标记抗体,Cy7标记抗体,PE标记抗体,PE-Cy3标记抗体,PE-Cy5标记抗体,PE-Cy5.5标记抗体,PE-Cy7标记抗体,APC标记抗体,AlexaFluor 350标记抗体,Alexa Fluor 488标记抗体,Alexa Fluor 555标记抗体,Alexa Fluor647标记抗体 抗体的交叉反应: 人,小鼠,大鼠,鸡 ,狗,猪,羊,牛,兔等...... 抗体的应用:可以用于做石蜡切片免疫组化,冰冻切片免疫组化,Elisa,WB,免疫荧光等实验。
突触相关蛋白25结合蛋白抗体规格实验原理 :
(1)特异性结合抗原:抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞,通常需要补体或吞噬细胞等共同发挥效应以清除病原微生物或导致病理损伤。然而,抗体可通过与病毒或毒素的特异性结合,直接发挥中和病毒的作用。
(2)活补体:IgM、IgG1、IgG2和IgG3可通过经典途径激活补体,凝聚的IgA、IgG4和IgE可通过替代途径激活补体。
(3)结合细胞:不同类别的免疫球蛋白,可结合不同种的细胞,参与免疫应答。
(4)可通过胎盘及粘膜:免疫球蛋白G(IgG)能通过胎盘进入胎儿血流中,使胎儿形成自然被动
免疫。免疫球蛋白A(IgA)可通过消化道及呼吸道粘膜,是粘膜局部抗感染免疫的主要因素。
(5)具有抗原性:抗体分子是一种蛋白质,也具有刺机体产生免疫应答的性能。不同的免疫球蛋白分子,各具有不同的抗原性。
(6)抗体对理化因子的抵抗力与一般球蛋白相同:不耐热,60~70℃即被破坏。各种酶及能使蛋白质凝固变性的物质,均能破坏抗体的作用。抗体可被中性盐类沉淀。在生产上常可用硫酸铵或硫酸钠从免疫血清中沉淀出含有抗体的球蛋白,再经透析法将其纯化。Anti-pre-X protein(CT)/FITC 荧光素标记抗乙肝病毒pre-X蛋白抗体(C端)IgGMulti-class antibodies规格: 0.2mlRabbit Anti-human IgG Whole serum 兔抗人IgG抗血清Multi-class antibodies规格: 1ml白介素12抗体 anti-IL-12 0.1mlDonkey anti-human IgG whole serum 驴抗人IgG抗血清 1mlphospho-FAK(Ser910) 英文名称: 化粘着斑激酶抗体 0.1mlRhesus antibody Rh phospho-PTPN6(Tyr536) 化蛋白酪酸酶1C抗体 规格 0.1mlRabbit Anti-human IgG Whole serum 兔抗人IgG抗血清Multi-class antibodies规格: 1ml
phospho-KLF5/Krueppel-like factor 5 (pSer272) 化肠道内富含的Kruppel样因子5抗原Multi-class antibodies规格: 0.5mgAnti-AFP 甲胎蛋白抗体Multi-class antibodies规格: 0.1mlRhesus antibody Rh phospho-Caspase 6 (Ser257) 化半胱胺酸蛋白酶蛋白6抗体 规格 0.1ml兔血清封闭液 10ml Gibco原料,2%血清VGF/NERP2 英文名称: 神经内分泌调节肽VGF抗体 0.1mlDNA polymerase delta p50 英文名称: DNA聚合酶δ2/DNA pol δ 2抗体 0.2mlAnti-AFP 甲胎蛋白抗体Multi-class antibodies规格: 0.1ml
STARD5 英文名称: 促黄体激素诱导蛋白5抗体 0.2mlEphB6 英文名称: 酪酸蛋白激酶受体B6抗体 0.1ml包含氧化还原酶的WW域抗体 Anti-WWOX 0.1mlAnti-TSH/FITC 荧光素标记促甲状腺素抗体IgGMulti-class antibodies规格: 0.2mlRhesus antibody Rh phospho-IKK beta(Ser476) 化KBYZ蛋白激酶β抗体 规格 0.1mlTranthyretin 甲状腺素运载蛋白(抗原)Multi-class antibodies规格: 0.5mg
AcetamideAgar假单胞F琼脂 250(g) incubation media 假单胞F琼脂 250(g)波尔顿肉汤 Bolton Selective Enrichment Broth 250克 弯曲杆菌的选择性增菌培养HE琼脂(HE)250用于沙门氏菌的选择性分离培养(FDA标准)incubationmediaHE琼脂(HE)250用于沙门氏菌的选择性分离培养(FDA标准)EF-18琼脂 250g 用于滤膜法检测食品中沙门氏菌(SN/T1059.1)
葡萄糖半固体培养基 250(g) incubation media 葡萄糖半固体培养基 250(g)动力--尿素培养基基础(MIU) 250(g) incubation media 动力--尿素培养基基础(MIU) 250(g)SIM培养基 250(g) incubation media SIM培养基 250(g)乳糖肉汤(LB) 250(g) incubation media 乳糖肉汤(LB) 250(g)
突触相关蛋白25结合蛋白抗体规格Bordet-GengouAgarMediumDMEM(低糖),液体 "含1000mg/l葡萄糖,L-谷酰胺, incubation media DMEM(低糖),液体 "含1000mg/l葡萄糖,L-谷酰胺,胶质芽孢杆菌 支/瓶NA平板(加青霉素酶)(9cm)细菌计数、不含糖,可作血琼脂基础和传代用XLDAgar