血小板源性生长因子受体B抗体(PDGFRβ)使用说明书

货号： BH-K21050 保存条件： Store at -20 °C 浓度： 1mg/1ml 应用范围： WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 形态： 大量 亚型： IgG 免疫原： KLH conjugated synthetic peptide derived from human PDGF-R-B C-terminus 适应物种： 详见说明书 宿主： 详见说明书 标记物： 详见说明书 抗体名： 血小板源性生长因子受体B抗体(PDGFRβ)使用说明书 抗体英文名： Anti-PDGF Receptor beta/PDGFRB 保质期： 详见说明书 抗原来源： Rabbit 克隆性： 多克隆 目录编号： 详见说明书 级别： 详见说明书 靶点： 详见说明书 数量： 大量 供应商： 上海博湖 规格： 0.2ml/200μg

中文名称  血小板源性生长因子受体B抗体(PDGFRβ)使用说明书
英文名称  Anti-PDGF Receptor beta/PDGFRB
别    名  CD140B; Beta platelet derived growth factor receptor; Beta-type platelet-derived growth factor receptor; CD 140B; CD140 antigen-like family member B; CD140B; CD140b antigen; JTK12; OTTHUMP00000160528; PDGF R beta; PDGF-R-beta; PDGFR; PDGFR1; PDGFRB; PGFRB
血小板源性生长因子受体B抗体(PDGFRβ)使用说明书是用于化学反应、分析化验、研究实验、教学实验、化学配方使用的纯净化学品，产品品质，价格实惠，多种规格供应，售后完善。

浓    度  1mg/1ml
规 格  0.1ml/100μg 0.2ml/200μg
抗体来源  Rabbit
克隆类型  polyclonal
交叉反应  Human, Mouse, Rat, Dog
产品类型  一抗  
研究领域  肿瘤 细胞生物 信号转导 细胞凋亡 生长因子和激素 细胞膜受体
蛋白分子量  predicted molecular weight: 118kDa 
性    状  Lyophilized or Liquid
免 疫 原  KLH conjugated synthetic peptide derived from human PDGF-R-B C-terminus
亚    型  IgG
纯化方法   affinity purified by Protein A
储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

血小板源性生长因子受体B抗体(PDGFRβ)使用说明书产品应用  WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复） 
 not yet tested in other applications.
 optimal dilutions/concentrations should be determined by the end user.  
保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 
Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
产品介绍 The PDGF Receptor Type A (Alpha platelet-derived growth factor receptor precursor, CD140a antigen), a 170kD protein, binds all three isoforms of PDGF with high affinity whereas the PDGF Receptor Type B, a 190kD protein, appears to bind only the PDGF BB homodimer with high affinity. Both receptors are transmembranous, ligand activated protein tyrosine kinases, which phosphorylate a number of important signal transduction proteins, which are bound with differential affinities via SH2 domains. The response of any given cell to PDGF will depend on the types of receptors displayed on the surface and isoforms of PDGF present in the extracellular environment.
Function : Receptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. Phosphorylates Tyr residues at the C-terminus of PTPN11 creating a binding site for the SH2 domain of GRB2.
Subunit : Interacts with homodimeric PDGFB and PDGFD, and with heterodimers formed by PDGFA and PDGFB. May also interact with homodimeric PDGFC. Monomer in the absence of bound ligand. Interaction with homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFD, leads to receptor dimerization, where both PDGFRA homodimers and heterodimers with PDGFRB are observed. Interacts with SH2B2/APS. Interacts directly (tyrosine phosphorylated) with SHB. Interacts (tyrosine phosphorylated) with PIK3R1. Interacts (tyrosine phosphorylated) with CBL. Interacts (tyrosine phosphorylated) with SRC and SRC family kinases. Interacts (tyrosine phosphorylated) with PIK3C2B, maybe indirectly. Interacts (tyrosine phosphorylated) with SHC1, GRB7, GRB10 and NCK1. Interaction with GRB2 is mediated by SHC1. Interacts (via C-terminus) with SLC9A3R1.
Subcellular Location : Cell membrane; Single-pass type I membrane protein. Cytoplasmic vesicle. Lysosome lumen. Note=After ligand binding, the autophosphorylated receptor is ubiquitinated and internalized, leading to its degradation.
N-glycosylated.
Ubiquitinated. After autophosphorylation, the receptor is polyubiquitinated, leading to its degradation.
DISEASE : Note=A chromosomal aberration involving PDGFRB is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with EVT6/TEL. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML).
Note=A chromosomal aberration involving PDGFRB may be a cause of acute myelogenous leukemia. Translocation t(5;14)(q33;q32) with TRIP11. The fusion protein may be involved in clonal evolution of leukemia and eosinophilia.
Note=A chromosomal aberration involving PDGFRB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with SPECC1.
Defects in PDGFRB are a cause of myeloproliferative disorder chronic with eosinophilia (MPE) [MIM:131440]. A hematologic disorder characterized by malignant eosinophils proliferation. Note=A chromosomal aberration involving PDGFRB is found in many instances of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;12) with ETV6 on chromosome 12 creating an PDGFRB-ETV6 fusion protein. Translocation t(5;15)(q33;q22) with TP53BP1 creating a PDGFRB-TP53BP1 fusion protein.
Note=A chromosomal aberration involving PDGFRB may be the cause of a myeloproliferative disorder (MBD) associated with eosinophilia. Translocation t(1;5)(q23;q33) that forms a PDE4DIP-PDGFRB fusion protein.
Note=A chromosomal aberration involving PGFRB is found in a patient with T-lymphoblastic lymphoma (T-ALL) and an associated myeloproliferative neoplasm (MPN) with eosinophilia. Translocation t(5;6)(q33-34;q23) with CEP85L. The translocation fuses the 5'-end of CEP85L (isoform 4) to the 3'-end of PDGFRB.
Similarity : Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
Database links : UniProtKB/Swiss-Prot: P09619.1
细胞膜受体（Membrane Receptors)
PDGF Receptor β


血小板源性生长因子受体B抗体(PDGFRβ)使用说明书具有全、新、优、品、好四大特点：
全：公司提供上万种产品，涵盖了生物试剂，elisa试剂盒，标准品，培养基，原装耗材，抗体、培养基、ATCC细胞等，基本上各种科研所需产品在我司都能找到。
新：产品更新速度较快，基本上每周都有新产品出现。
优：产品质量好，投诉比较少。
好：我公司具有优质的技术团队，产品一旦售出，实验过程中遇到困难可提供在线技术咨询。使您使用产品时没有任何的后顾之忧。
我们提供的标记服务，可根据您科研的需求，满足您的需要，质量保证，价格合理。
一、标记流程
委托标记服务，请下载并详细填写《委托标记申请单》，待我公司标记室技术人员研究、确认后答复，并办理委托标记事宜。
二、各种标记物制备的收费标准（RMB） 
我们不但提供抗体和大分子蛋白的标记服务，还提供小分子多肽及小分子蛋白的各种标记服务，提供标记化合物的服务（此化合物必须适用于标记），价格另议。
一抗和二坑的区别：
抗体就是平常所说的抗体，即能和抗原特异性结合。
第二抗体是能和抗体结合的，即抗体的抗体。主要用于检测抗体的存在。
一抗是针对抗原的抗体，二抗是针对一抗的抗体。即抗体也可以充当抗原刺激机体产生抗体。也就是说，抗原进入机体刺激机体免疫系统产生免疫应答，由B细胞可以产生与相应抗原发生特异性结合的特殊蛋白质。
一抗二抗都是一种可以特异结合别的物质的基团，而且一抗可以至少结合两种其他基团（底物和二抗）。
一抗：可以特异结合底物，就是识别出我们想要检测的东西。一抗和底物结合与否用肉眼是看不出来的。
二抗：可以和一抗结合，并带有可以被检测出的标记(如带荧光、放射性、化学发光或显色基团），作用是检测一抗。 如果一抗自己带有可以被检测出的标记(如带荧光、放射性、化学发光或显色基团），则不需要二抗。但这样成本很高，因为一种一抗只识别一种底物。所以如今的设计一般是二抗带上可检测标记，再来检测一抗。而一抗识别底物。这样，当一抗结合到底物上，就可以通过二抗检测出来。 

胆囊体  询价
胆囊颈  询价
胆囊疾病组织芯片  询价
胆囊管  询价
单组份BCIP/NBT显色试剂  询价
9号染色体开放阅读框抗体  Anti-C9orf  WB IHC-P IHC-F IF  100ul
9号染色体开放阅读框41抗体  Anti-C9orf41  WB IHC-P IHC-F IF  100ul
9号染色体开放阅读框5抗体  Anti-C9orf5  WB IHC-P IHC-F IF  100ul
9号染色体开放阅读框50抗体  Anti-C9orf50  WB IHC-P IHC-F IF  100ul
9号染色体开放阅读框57抗体  Anti-C9orf57  WB IHC-P IHC-F IF  100ul
N-乙基顺丁烯二酰亚胺敏感融合蛋白抗体  Anti-NSF  WB IHC-P IHC-F IF  100ul
NIPAL2蛋白抗体  Anti-NIPAL2  WB IHC-P IHC-F IF  100ul
神经营养因子HNT抗体  Anti-Neurotrimin  WB IHC-P IHC-F IF  100ul
轴突蛋白1β/Neurexin 1β抗体  Anti-Neurexin 1 beta  WB IHC-P IHC-F IF  100ul
谷酸受体3A抗体  Anti-NMDAR3A  WB IHC-P IHC-F IF  100ul
293T(人胚肾细胞) 5×106cells/瓶×2
CL-0306SMC-1(人胸膜瘤细胞)5×106cells/瓶×2
HA Others H7N9 甲型流感 H7N9 (A/Pigeon/Shanghai/S1069/2013) 血凝素HA1 (Hemagglutinin) 人细胞裂解液 (阳性对照)
A673细胞，横纹肌癌细胞 人巨核细胞保白血病细胞,Dami细胞 人恶性多发性畸胎瘤细胞;ERA-2
人胚肺二倍体细胞;KMB-17
PVR Others Mouse 小鼠 CD155 / PVR
人肝内胆管上皮细胞HIBEpiC
SIRPA Others Rat 大鼠 SIRP alpha / CD172a 人细胞裂解液 (阳性对照)
水牛皮肤成纤维样细胞;WB-S2
NCI-H292细胞，人肺癌细胞(淋巴结转移) 小鼠成纤维细胞,3T3细胞 CM-R071大鼠输尿管上皮细胞完全培养基100mL
RF/6A(猴视网膜血管内皮细胞) 5×106cells/瓶×2
Gibco 12680013 LHC-9 Medium (1X), Liquid 500 ml
血小板源性生长因子受体B抗体(PDGFRβ)使用说明书293T(人胚肾细胞) 5×106cells/瓶×2
CL-0306SMC-1(人胸膜瘤细胞)5×106cells/瓶×2
HA Others H7N9 甲型流感 H7N9 (A/Pigeon/Shanghai/S1069/2013) 血凝素HA1 (Hemagglutinin) 人细胞裂解液 (阳性对照)
A673细胞，横纹肌癌细胞 人巨核细胞保白血病细胞,Dami细胞 人恶性多发性畸胎瘤细胞;ERA-2
人胚肺二倍体细胞;KMB-17
PVR Others Mouse 小鼠 CD155 / PVR