货号:
BH-K21009
保存条件:
Store at -20 °C
浓度:
1mg/1ml
应用范围:
WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
形态:
大量
亚型:
IgG
免疫原:
KLH conjugated synthetic peptide derived from human ENPP1 N-terminus
适应物种:
详见说明书
宿主:
详见说明书
标记物:
详见说明书
抗体名:
核苷酸内焦磷酸酶/磷酸二酯酶1抗体价格
抗体英文名:
Anti-ENPP1/PC1/3
保质期:
详见说明书
抗原来源:
Rabbit
克隆性:
多克隆
目录编号:
详见说明书
级别:
详见说明书
靶点:
详见说明书
数量:
大量
供应商:
上海博湖
规格:
0.2ml/200μg
英文名称 Anti-ENPP1/PC1/3
中文名称 核苷酸内焦磷酸酶/磷酸二酯酶1抗体价格
别 名 ENPP1; ENPP 1; ENPP-1; PC1; PC-1; ectonucleotide pyrophosphatase/phosphodiesterase 1; Plasma cell membrane glycoprotein PC 1; Phosphodiesterase I/nucleotide pyrophosphatase 1; BDP; EC; NEC1; PC1; PC3; Pcsk1; Prohormone convertase 1; Prohormone convertase
核苷酸内焦磷酸酶/磷酸二酯酶1抗体价格浓 度 1mg/1ml
规 格 0.1ml/100μg 0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Rabbit
产品类型 一抗
研究领域 肿瘤 免疫学 信号转导 激酶和磷酸酶
蛋白分子量 predicted molecular weight: 100kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human ENPP1 N-terminus
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
核苷酸内焦磷酸酶/磷酸二酯酶1抗体价格产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 ENPP1 has a broad specificity and cleaves a variety of substrates, including phosphodiester bonds of nucleotides and nucleotide sugars and pyrophosphate bonds of nucleotides and nucleotide sugars. It can hydrolyze nucleoside 5' triphosphates such as ATP, GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate. It can also hydrolyze diadenosine polyphosphates and 3',5'-cAMP to AMP. It may play a role in the regulation of pyrophosphate production, the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling.The subtilisin-like Prohormone Convertase (PC) family is a group of cellular enzymes that cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. PC family members share structural similarities, which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. The subcellular localization of PC family members varies. Immunolocalization studies show that PC1 is found in the perinuclear region as well as the trans-Golgi network, whereas PC2 can be found in the trans-Golgi network as well as diffusely distributed in the peripheral cytoplasm.Function : Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin and insulin.Subunit : Homodimer; disulfide-linked.Tissue Specificity : Expressed in plasma cells and also in a number of non-lymphoid tissues, including the distal convoluted tubule of the kidney, chondrocytes and epididymis.DISEASE : Defects in PCSK1 are the cause of proprotein convertase 1 deficiency (PC1 deficiency) [MIM:600955]. PC1 deficiency is characterized by obesity, hypogonadism, hypoadrenalism, reactive hypoglycemia as well as marked small-intestinal absorptive dysfunction It is due to impaired processing of prohormones.Similarity : Belongs to the peptidase S8 family. Furin subfamily.
核苷酸内焦磷酸酶/磷酸二酯酶1抗体价格抗体的鉴定:
1)抗体的效价鉴定:不管是用于诊断还是用于ZL,制备抗体的目的都是要求较GX价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。
2)抗体的特异性鉴定:抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争YZ试验测定。以不同浓度抗原和近似抗原分别做竞争YZ曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。
如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。
3)亲和力:是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。
种属:
兔抗单克隆抗体,鼠抗单克隆抗体。兔抗多克隆抗体。抗体的浓度为1mg/ml。 抗体及相关标记抗体:HRP标记抗体,Biotin标记抗体,Gold标记抗体,RBITC标记抗体,AP标记抗体,FITC标记抗体,Cy3标记抗体,Cy5标记抗体,Cy5.5标记抗体,Cy7标记抗体,PE标记抗体,PE-Cy3标记抗体,PE-Cy5标记抗体,PE-Cy5.5标记抗体,PE-Cy7标记抗体,APC标记抗体,AlexaFluor 350标记抗体,Alexa Fluor 488标记抗体,Alexa Fluor 555标记抗体,Alexa Fluor647标记抗体 抗体的交叉反应: 人,小鼠,大鼠,鸡 ,狗,猪,羊,牛,兔等...... 抗体的应用:可以用于做石蜡切片免疫组化,冰冻切片免疫组化,Elisa,WB,免疫荧光等实验。
核苷酸内焦磷酸酶/磷酸二酯酶1抗体价格实验原理 :
(1)特异性结合抗原:抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞,通常需要补体或吞噬细胞等共同发挥效应以清除病原微生物或导致病理损伤。然而,抗体可通过与病毒或毒素的特异性结合,直接发挥中和病毒的作用。
(2)活补体:IgM、IgG1、IgG2和IgG3可通过经典途径激活补体,凝聚的IgA、IgG4和IgE可通过替代途径激活补体。
(3)结合细胞:不同类别的免疫球蛋白,可结合不同种的细胞,参与免疫应答。
(4)可通过胎盘及粘膜:免疫球蛋白G(IgG)能通过胎盘进入胎儿血流中,使胎儿形成自然被动
免疫。免疫球蛋白A(IgA)可通过消化道及呼吸道粘膜,是粘膜局部抗感染免疫的主要因素。
(5)具有抗原性:抗体分子是一种蛋白质,也具有刺机体产生免疫应答的性能。不同的免疫球蛋白分子,各具有不同的抗原性。
(6)抗体对理化因子的抵抗力与一般球蛋白相同:不耐热,60~70℃即被破坏。各种酶及能使蛋白质凝固变性的物质,均能破坏抗体的作用。抗体可被中性盐类沉淀。在生产上常可用硫酸铵或硫酸钠从免疫血清中沉淀出含有抗体的球蛋白,再经透析法将其纯化。
会厌 询价回肠 询价喉肿瘤 询价喉部粘膜 询价红细胞裂解液 询价
B淋巴细胞特异性激活OCT结合蛋白1抗体 Anti-BOB1 WB IHC-P IHC-F IF 100ul核糖体合成蛋白BOP1抗体 Anti-BOP1 WB IHC-P IHC-F IF 100ul调节ZX体分离早期相关激酶BORA抗体 Anti-BORA WB IHC-P IHC-F IF 100ul莱姆病螺旋体抗体 Anti-Borrelia burgdorferi WB IHC-P IHC-F IF 100ul精子成熟相关蛋白BOULE抗体 Anti-BOULE WB IHC-P IHC-F IF 100ul
烟酰胺腺嘌呤二核苷酸酶1抗体 Anti-Nmnat1 WB IHC-P IHC-F IF 100ul合成酶-1抗体 Anti-nNOS WB IHC-P IHC-F IF 100ul转录因子相关蛋白NKX6.1抗体 Anti-NKX6.1 WB IHC-P IHC-F IF 100ul造血蛋白1抗体 Anti-NCKAP1L WB IHC-P IHC-F IF 100ul骨巢蛋白抗体 Anti-Nidogen2 WB IHC-P IHC-F IF 100ul
非洲绿猴肾细胞系/HCV-E2;Vero-HCV-E2HCT116, 人结直肠癌细胞NRG1 Others Canine 狗 NRG1-alpha (ECD) 人细胞裂解液 (阳性对照)SW 13细胞,肾上腺皮质瘤细胞 人子细胞,C-33A细胞 人骨骼肌成肌细胞总RNAHSkMM NA615小鼠T细胞性白血病瘤株;L7712FN1 Others Human 人 Fibronectin / Fibronectin Fragme 2 人细胞裂解液 (阳性对照)
人虹膜色素上皮细胞HIPEpiCEDA2R Others Rat 大鼠 XEDAR / EDA2R 人细胞裂解液 (阳性对照)大鼠肠动脉内皮细胞完全培养基 100mLCCRF-CEM [CCRF CEM]人急性淋巴细胞白血病T淋巴细胞 The CCRF-CEM [CCRF CEM] human acute lymphoblastic leukemia T lymphocytes 16+10%Hyclone 灭HX清EGF Protein Human 重组人 EGF / Epidermal Growth Factor 蛋白 (Fc 标签)293ET(人胚肾细胞(SVT和EBNA1基因修饰细胞)) 5×106cells/瓶×2 原代心肌细胞特制基础培养基Many types of cells包装:500/250/100ml
核苷酸内焦磷酸酶/磷酸二酯酶1抗体价格非洲绿猴肾细胞系/HCV-E2;Vero-HCV-E2HCT116, 人结直肠癌细胞NRG1 Others Canine 狗 NRG1-alpha (ECD) 人细胞裂解液 (阳性对照)SW 13细胞,肾上腺皮质瘤细胞 人子细胞,C-33A细胞 人骨骼肌成肌细胞总RNAHSkMM NA615小鼠T细胞性白血病瘤株;L7712FN1 Others Human 人 Fibronectin / Fibronectin Fragme 2 人细胞裂解液 (阳性对照)