Anti-Cytochrome b561 D1抗体

Anti-Cytochrome b561 D1抗体Myc-Tag单克隆抗体
Size
200ug/100ug/50ug
Source
Mouse
Immunogen
Synthetic Peptide
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Specificity
The antibody can detect Myc fusion proteins.
Formulation
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Concentration
1 mg/ml
Storage / Stability
-20°C/1 year
Reactivity
N/A
Applications
WB, IP, IF
Dilution
Anti-Cytochrome b561 D1抗体WB: 1:5000 IP: 1:200 IF: 1:1000
Myc-Tag Monoclonal Antibody Images

IP antibody use: 3ug Flag Mouse IgG1 per ml Lysate, WB 1: 5000 1) untransfected 293 cell lysate 2) transfected 293 cell lysate with Myc-Tag fusion protein 3) IP (transfected 293+ normal Mouse IgG+Protein G agarose) 4) IP (transfected 293+anti- C-Myc m

IF analysis of 293 cells transfected with a Myc-tag protein,1:2000 dilution (blue DAPI, red anti-Myc)

1ug Myc fusion protein+ Primary antibody dilution at 1) 1:5000 2) 1:10000
Sample: 
Spleen (Mouse) Lysate at 40 ug
RAW264.7 Cell (Mouse) Lysate at 40 ug
Primary: Anti-Cytochrome b561 D1抗体 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 26 kD