Anti-H3K4me2抗体

Anti-H3K4me2抗体Myc-Tag单克隆抗体
Size
200ug/100ug/50ug
Source
Mouse
Immunogen
Synthetic Peptide
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Specificity
The antibody can detect Myc fusion proteins.
Formulation
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Concentration
1 mg/ml
Storage / Stability
-20°C/1 year
Reactivity
N/A
Applications
WB, IP, IF
Dilution
Anti-H3K4me2抗体WB: 1:5000 IP: 1:200 IF: 1:1000
Myc-Tag Monoclonal Antibody Images

IP antibody use: 3ug Flag Mouse IgG1 per ml Lysate, WB 1: 5000 1) untransfected 293 cell lysate 2) transfected 293 cell lysate with Myc-Tag fusion protein 3) IP (transfected 293+ normal Mouse IgG+Protein G agarose) 4) IP (transfected 293+anti- C-Myc m

IF analysis of 293 cells transfected with a Myc-tag protein,1:2000 dilution (blue DAPI, red anti-Myc)

1ug Myc fusion protein+ Primary antibody dilution at 1) 1:5000 2) 1:10000
Anti-H3K4me2抗体注意事项：
1.抗体保存原则：避免反复冻融
2.短期保存的抗体：如抗体几周内就会使用，收到后推荐在2-8℃保存，2-8℃短暂保存数周对抗体活性没有影响。
3.长期保存的抗体：如抗体需超过1个月后才会使用，请酌情分装后于-20℃保存。
4.建议：将抗体保存在冰箱里层，避免温度变化造成反复冻融。
避免将抗体保存在自动除霜冰箱中。
反复冻融过程中，冰晶会对抗体的空间结构造成破坏，导致蛋白变性形成多聚体，从而降低抗体的结合能力，加快抗体降解速度。