Mouse CSPG4 / NG2 ELISA Kit (Sandwich ELISA) - LS-F35054

Mouse CSPG4 / NG2 ELISA Kit (Sandwich ELISA) - LS-F35054
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LS-F35054-1
1 plate
$1,090 USD
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SpecificationsPublicationsReviewsImagesPopular CSPG4 / NG2 Elisa Kits
Rat CSPG4 / NG2 ELISA Kit (Sandwich ELISA) - LS-F19336
Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Rat
Range: 0.312-20 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma

Human CSPG4 / NG2 ELISA Kit (Sandwich ELISA) - LS-F6503
Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Human
Range: 0.156-10 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Lysates, Tissue Homogenates

Mouse CSPG4 / NG2 ELISA Kit (Sandwich ELISA) - LS-F11232
Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Mouse
Range: 9.38-600 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma

Human CSPG4 / NG2 ELISA Kit (Sandwich ELISA) - LS-F11233
Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Human
Range: 0.156-10 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma

Human CSPG4 / NG2 ELISA Kit (Sandwich ELISA) - LS-F21262
Type: Quantitative Sandwich ELISA
Format: 96-Well Microplate
Reactivity: Human
Range: 0.156-10 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma

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Product DescriptionLS-F35054 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse CSPG4 / NG2 in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of CSPG4 / NG2 as low as 0.094 nanograms per millilter.
About CSPG4 / NG2
Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. Uniprot: Q6UVK1 NCBI: NM_001897 NP_001888.2

Synonyms: CSPG4 ElisaKit, MCSP ElisaKit, MEL-CSPG ElisaKit, MSK16 ElisaKit, NG2 ElisaKit, HMW-MAA ElisaKit, MCSPG ElisaKit

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SpecificationsType
 
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
 
CSPG4 / NG2
Reactivity
 
Mouse
Manual
 

Intended Sample Types
 
Plasma, Serum, Tissue Homogenates
Format
 
96-Well Strip Plate
Detection
 
Colorimetric - 450nm (TMB)
Measurement
 
Quantitative
Detection Range
 
0.156 - 10 ng/ml
Sensitivity
 
0.094 ng/ml
Precision
 
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
 
Store at 4°C. Stable for 6 months.
Quality Assurance
 
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
 

• Adhesive Plate Sealers
• Coated 96-well Strip Plate
• Detection Antibody Diluent
• Biotinylated Detection Antibody (100x)
• HRP-Streptavidin Conjugate (100x)
• HRP-Streptavidin Conjugate Diluent
• Sample Diluent
• TMB Substrate
• Stop Solution
• Wash Buffer (25x)
• Standard (Lyophilized)

Background
 
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Cspg4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Cspg4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Cspg4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofCspg4 can be calculated.
Restrictions
 
For research use only.
Guarantee
 
This elisa kit carries the LSBio Guarantee.
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