Goat CRP / C-Reactive Protein ELISA Kit (Sandwich ELISA) - LS-F31855

Goat CRP / C-Reactive Protein ELISA Kit (Sandwich ELISA) - LS-F31855
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LS-F31855-1
1 plate
$695 USD
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SpecificationsPublicationsReviewsImagesPopular CRP / C-Reactive Protein Elisa Kits
Rat CRP / C-Reactive Protein ELISA Kit (Sandwich ELISA) - LS-F9616
Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Rat
Range: 15.63-1000 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Culture Supernatants, Cell Lysates, Serum, Tissue Homogenates, Plasma

Canine CRP / C-Reactive Protein ELISA Kit (Sandwich ELISA) - LS-F32739
Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Canine
Range: 0.313-20 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Tissue Homogenates, Plasma

Mouse CRP / C-Reactive Protein ELISA Kit (Sandwich ELISA) - LS-F10439
Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Mouse
Range: 0.02-2 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Plasma

Guinea pig CRP / C-Reactive Protein ELISA Kit (Sandwich ELISA) - LS-F5561
Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Guinea pig
Range: 15.63-1000 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma

Rabbit CRP / C-Reactive Protein ELISA Kit (Sandwich ELISA) - LS-F32738
Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Rabbit
Range: 0.781-50 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Tissue Homogenates, Plasma

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Product DescriptionLS-F31855 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Goat CRP / C-Reactive Protein in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of CRP / C-Reactive Protein as low as 0.938 nanograms per millilter.
About CRP / C-Reactive Protein
Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. Uniprot: P02741 NCBI: NM_000567 NP_000558.2

Synonyms: CRP ElisaKit, C-reactive protein ElisaKit, Pentraxin 1 ElisaKit, PTX1 ElisaKit

More CRP / C-Reactive Protein Products
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SpecificationsType
 
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
 
CRP / C-Reactive Protein
Reactivity
 
Goat
Manual
 

Intended Sample Types
 
Plasma, Serum, Tissue Homogenates
Format
 
96-Well Strip Plate
Detection
 
Colorimetric - 450nm (TMB)
Measurement
 
Quantitative
Detection Range
 
1.563 - 100 ng/ml
Sensitivity
 
0.938 ng/ml
Precision
 
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
 
Store at 4°C. Stable for 6 months.
Quality Assurance
 
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
 

• Coated 96-well Strip Plate
• Detection Antibody Diluent
• Biotinylated Detection Antibody (100x)
• HRP-Streptavidin Conjugate (100x)
• HRP-Streptavidin Conjugate Diluent
• Sample Diluent
• TMB Substrate
• Stop Solution
• Wash Buffer (25x)
• Standard (Lyophilized)
• Adhesive Plate Sealers

Background
 
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- CRP antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-CRP antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CRP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CRP can be calculated.
Restrictions
 
For research use only.
Guarantee
 
This elisa kit carries the LSBio Guarantee.
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