RAW264.7[RAW264.7]细胞 小鼠单核巨噬细胞系更多培养技术指导

RAW264.7[RAW264.7]细胞 小鼠单核巨噬细胞系更多培养技术指导 货号：GD-C19819179

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞 全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息，我们拥有丰富的细胞系培养经验，能提供细胞培养全方位的技术支持，让您实验再无烦扰！公司由在国外工作和学习多年的留学人员创办，由国内ZG科学院上海生物化学与细胞生物学研究所，复旦大学，同济大学等多名工作在线的细胞培养专家，提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念，以世界的技术支撑阵容，提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美，提供了重要的基石；公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品，立足国内，面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位，为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内，在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室，以便更好的服务国内广大医院、大学、制药企业等，你们的每一份关心和支持，都是我们前进的动力！
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，原因比较复杂，常见原因可归纳为：培养基使用错误或培养基品质不佳；血清使用错误或血清的品质不佳；解冻过程错误；冷冻细胞解冻后，加以洗涤细胞和离心；悬浑细胞误认为死细胞；培养温度使用错误；细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来，其离心速率应为多少转速?欲回收动物细胞 其离心速率一般为300×g(约1OOOrpm),5-10分钟，转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2，其中r为离心机转轴ZX与离心套管底部内壁的距离；rpm为离心机每分钟的转数；RCF(relative centrifugal force)为相对离心力，以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
4-Nitrobenzenesulfonyl chloride, 97%
Benzyl (S,S,S)-2-azabicyclo[3.3.0]octane-3-carboxylate hydrochloride
4-ForMylfuran-2-boronic acid
N,N'-Di-2-naphthyl-1,4-phenylenediaMine , 96%
2-BROMO-5-NITROTOLUENE
2-(2-(tert-ButyldiMethylsilyloxy)ethyl)phenylboronic acid
4-(3-Methylbutanoyl)phenylboronic acid
Phenylselenocyanate, tech., 90%
(R)-4-Formyl-2,2-dimethyl-oxazolidine-3-carboxylicacid tert-butyl ester
2-(chloromethyl)-4-methoxypyridine(SALTDATA: 1.02HCl 0.19H2O)
5-Methyl-2-nitrobenzaldehyde
Boc-L-Glutamic acid
Phosmet
BENZOYL BROMIDE
trans-Ethyl cinnaMate
2-BroMo-1-(1-Methyl-1H-benziMidazol-2-yl)-1-ethanone
(1R,2R)-N,N'-Dihydroxy-N,N'-bis(diphenylacetyl)cyclohexane-1,2-diaMine
2,4-DiMethylaniline-5-sulfonic Acid SodiuM Salt Hydrate
Undecylbenzene, 99%
SodiuM Hypochlorite Solution, 5 Percent Available Chlorine, 5 Percent Available Chlorine, Solution, Reagent, ACS
4-(TrifluoroMethoxy)phenol
Diethyl fumarate
2-Chloro-1-phenyl-1-propanone
4-Chlorostyrene
4-fluoro-3-iodotoluene
2,7-Dichlorofluorene
4-Ethylaniline
2-AMINO-6-HYDROXYPYRIDINE
5-Chloro-3-Methylbenzo[b]thiophene, 97%
6-Cyano-2-fluorobenzeneboronic acid pinacol ester, 96%
2-AMino-5-Methoxybenzoic acid
3,5,5-TriMethylhexanoic Acid
N-2-TrifluoroMethylphenyl 4-boronobenzaMide
PERFLUORO-2,5,8,11-TETRAMETHYL-3,6,9,12-TETRAOXAPENTADECANOYL FLUORIDE
N-EPSILON-FORMYL-L-LYSINE
CHLORODIMETHYL-3,3,3-TRIFLUOROPROPYLSILANE
4-Bromo-1,1,2-trifluorobut-1-ene 98%
6-Chloropyridazine-3-carbonitrile
2-Amino-6-chloronicotinic acid
N-(4-Chloropyridin-2-yl)acetamide ,97%
Brompheniramine
(S)-(-)-2,2'-Bis(diphenylphosphino)-1,1'-binaphthyl
4-Fluoro-3-nitrophenylboronic acid
EC 3.2.1.31
5-BroMo-2-picoline
Sulfobetaine 8
2,4,6,8-TetrachloropyriMido[5,4-d]pyriMidine
2-BroMo-6-Methoxynaphthalene, 98%
(S)-3-AMino-3-phenylpropan-1-ol, 95%, 98% ee
1-Phenethyl-4-piperidone
4'-Hexylacetophenone
Ferrosoferric oxide
Reactive Scarlet GF
Sodium 3-(benzothiazol-2-ylthio)-1-propanesulfonate
2-phenyl-1H-imidazole-1-propiononitrile
1,2-Bis(2-Nitrophenoxy)ethane
3-Nitro-N-Methylaniline
3-AMino-3-(2-nitrophenyl)propanoic acid
2,3-DiMethylbroMobenzene
2,4-Difluorophenol
1-(p-Toluenesulfonyl)pyrrole
(+)-Bicuculline
4-Phenylcinnamic acid
2-Aminofluorene
3-Cyano-4,6-dimethyl-2-hydroxypyridine
Potassium chloroplatinate
2,2-Dimethylbutyric acid
4-Iodobenzoyl chloride
1-Benzyl-3-(tert-butoxycarbonylaMino)pyrrolidine
Ethyl 5-chloro-8-Methyl-4-oxo-1,4-dihydroquinoline-3-carboxylate
Allylboronic acid pinacol ester, 98%
O,O-Bis(diethoxyphosphoryl)calix[4]arene, 97%
5-Iodosalicylic Acid
2-BroMo-6-(trifluoroMethyl)pyridine
2-Tridecanone
3-methylazetidin-3-ol
2,3,5,6-Tetrafluoro-1,4-benzenedimethanamine
2-methylpyridine-4-carbaldehyde
GSK ELISA Kit
TAU ELISA Kit
Human apoprotein A1，apo-A1 ELISA Kit
Human ARSE ELISA Kit
Chicken Interleukin 2,IL-2 ELISA Kit
α2-MG ELISA Kit
PGC-1α ELISA Kit
Human monocyte chemotactic protein 3,MCP-3 ELISA Kit
Human VASH2 ELISA Kit
HMG-CoA ELISA Kit
Bromcresol Purple Glucose Peptone Water Medium
Zobell Marine Agar 2216
Iron yttriuM oxide, 99.9% (REO)
PotassiuM Hydrogen Fluoride
5-ALPHA-ANDROSTANE
2-Fluoropyridine-5-boronic acid
Diethyl fumarate
2-phenylbutyrophenone
NBD chloride
3-fluorobenzyl alcohol

5-Methylisoxazole-3-carboxylic acid
3-Hydroxy-4-nitrobenzoic acid
TelluriuM(IV) broMide, 99.9% (Metals basis)
1-(TrifluoroMethyl)vinyl acetate, 97%
3-(TrifluoroMethyl)crotonic acid, 96%
HydroxypropylMethylcellulose
o-ChlorobenzenesulfonaMide
4-Methyl-2-nitrophenylboronic acid
DiMethylsulfaMoyl chloride
CHLOROMETHYL P-TOLYL SULFIDE
trans-Bis(dicyclohexylaMine)palladiuM(II) acetate
Metoclopramide hydrochloride ,99%
2-Thiophenemethanol, 99+%
Dimethylammonium dichlorotri(?chloro)bis{(R)-(+)-2,2'-bis[di(3,5-xylyl)phosphino]-1,1'-binaphthyl}diruthenate(II) [NH2Me2][{RuCl((R)-xylbinap)}2(?Cl)3]
3-Fluoroazetidine hydrochloride