DMS-153细胞 人肺癌细胞系更多培养技术支持 货号:GD-C19720514
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞 全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤:【瘤细胞悬液接种】1)无菌选取生长良好(有光泽,淡红色)瘤组织或对数生长期培养瘤细胞;2)在PBS中将瘤组织剪碎后用匀浆器研磨,经80~100目筛网过滤成细胞悬液;3)培养细胞应用PBS洗两遍;4)计数并调整细胞浓度至107~108/ml;5)常规消毒后,于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位,>106细胞)。初次接种成功率低,细胞数尽可能多一些;6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期,以后随着传代潜伏期逐渐缩短,固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位,引起腹水,腹水中含有瘤细胞 将这种腹水反复传代,即可成为腹水瘤。初次传代时,腹水常呈血性(含大量红细胞),反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤,进行细胞计数;2)消毒动物,左下腹穿刺接种106腹水瘤细胞;3)接种腹水瘤细胞后约7~12d,待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部,用9号针头抽取腹水,也可行腹部解剖后,用滴管吸取。每只小鼠可抽3~5ml;4)抽取的腹水经3000rpm离心15min,收集上清,分装冻存备用。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
2-Methoxy-4-(trifluoroMethoxy)benzaldehyde, 97%
2-(TrifluoroMethyl)-D-phenylalanine
4-DIAZO-N-ETHYL-N-(2-HYDROXYETHYL)ANILINE CHLORIDE ZINC CHLORIDE
2-BroMophenylhydrazine hydrochloride
6-(HydroxyMethyl)pyridine-3-boronic acid
1,5-DICHLOROHEXAMETHYLTRISILOXANE
Ammonium hexafluorogermanate, 99%
1-BOC-3-Azetidinol
L-Alanine tert-butyl ester hydrochloride
3-Decanone
3-methyl-4-(1H-pyrrol-1-yl)benzoic acid(SALTDATA: FREE)
Diphenylphosphoryl azide
4-Pentylphenyl 4-propylbenzoate
Fmoc-p(NH-Boc)-L-Phe-OH
2-Methyl-2H-indazole-3-carboxylic acid
Squalene
DiMethyldioctadecylaMMoniuM Iodide
4-(Morpholine-4-carbonyl)broMobenzene
4-n-Butylphenylboronic acid, 95%
TolMetin SodiuM Salt, Dihydrate, USP
DiMethyldistearylaMMoniuM Chloride
4,6-Dimethoxysalicylaldehyde
methyl 3-hydroxy-4-methoxybenzoate
C.I. Acid Orange 24
4-Isopropyl Benzoic Acid
ETHYL PHENYL SULFIDE
3-Iodobenzyl bromide
3-Chloropropanesulfonyl chloride
2-Chloro-5-MethoxybenziMidazole, 94%
3-Methylthiophene-2-carbonitrile, 96%
Boc-L-alanine
5-BroMoindole-2-carboxylic Acid
DL-6-METHOXY-ALPHA-METHYL-2-NAPHTHALENEMETHANOL
N-Methyl-N-nitrosotoluene-4-sulphonaMide
4-Carboxybenzenesulfonazide
2-(3-Methoxyphenyl), 95%
Dibenzyl phosphite ,98%
N-Allyl glycine hydrochloride
2,5-Dimethyl-1-(2-thienylmethyl)-1H-pyrrole-3-carboxylic acid , 97%
N-(Butoxymethyl)acrylamide (stabilized with MEHQ)
5-Iodo-2-furancarboxaldehyde
4-((2-Hydroxyethyl)amino)-3-nitrophenol
Cobalt(II) fluoride
1,2,5,6-Dibenzanthracene
Tetraethyl orthosilicate
4-(Dibenzofuranyl)boronic acid
Methyl Nonafluorobutyl Ketone
Z-MeAla-OH
2-AMino-3-broMo-5-nitropyridine, 98%
Nitrapyrin
1-Acenaphthenol
N-Acetyl-2-oxindole
Quinoline-7-carbaldehyde
2,2-Dimethyl-1-propanamine hydrochloride (1:1)
Hydrazine acetate
Oxobutanedioic acid
6-AMinoerythrosin
4-ChloroMethyl-2,2-diMethyl-1,3-dioxolane
1,4-DIBROMOOCTAFLUOROBUTANE
4-Benzyloxy-1-butanol, 97%
Chromane-2-carboxylic acid, 97%
(3-Oxo-2,3-dihydro-1 H -isoindol-1-yl)-acetic acid
3,6-Di-tert-butylcarbazole
4,6-DIMETHYLPYRIMIDINE
2-CHLORO-4,5-DIFLUOROBENZENESULFONYL CHLORIDE
3-Hydroxy-N-2-naphthyl-2-naphthamide
2-Ethylpyridine
{2-[(4-Methylpiperazin-1-yl)Methyl]phenyl}MethylaMine
1-Ethoxy-2-propanol
Nevirapine
N-Methyl 4-aMinobenzenesulfonaMide
2,4,6-TriMethoxybenzylaMine hydrochloride, 98%
4,5-Dimethylthiazole
Maleic Acid
3-(4-Methoxyphenoxy)pyrrolidine hydrochloride
3-(2-Naphthyl)acrylic acid
2-methoxyphenylmagnesium bromide
bis(trimethylsilyloxy)methyl-methoxy-silane
GDF9 ELISA Kit
MAPKAPK2 ELISA Kit
Human Free Protein S,FP-S ELISA Kit
Human catecholamine,CA ELISA Kit
TNF-a ELISA Kit
ACTg1 ELISA Kit
LEP ELISA Kit
PKC ELISA Kit
Human THBS4 ELISA Kit
LAM ELISA Kit
Cary-Blair Transport Medium
Streptococcus mutans Medium
VINYLACETIC ACID
Kinetin, 99%
5-(2-Pyridyl)-1,3-oxazole, 97%
2-Iodopyridine-4-carboxylic acid, 95%
4,6-Dimethoxysalicylaldehyde
1-(3-BROMOPHENYL)TETRAHYDRO-2H-IMIDAZOL-2-ONE
Solvent Black 34
4-Iodophenol
1,4-Dibromo-2,5-dimethylbenzene
5-HEXENOIC ACID
1-(4-BroMophenyl)Hexane
Tungsten boride, 99.4% (Metals basis)
4-(trans-3-Methoxy-3-oxo-1-propen-1-yl)benzeneboronic acid, 97%
2-BroMotoluene, 99%
FMoc-Lys(Ddiv)-OH
METHYLDICHLOROPHOSPHINE
3-METHYLFORMANILIDE
2-BroMo-4-fluorophenol
3-(TrifluoroMethyl)benzhydrol, 97%
5-BroMo-4-(3,4-diMethylphenyl)pyriMidine
2,4,6-Tribromophenyl Hexanoate
2-Amino-3-methyl-5-nitropyridine
4-Butyl-2-methylphenyl isocyanate, tech