NCI-H1666[H-1666,H1666]细胞 人肺支气管癌细胞系更多培养技术支持 货号:GD-C19720461
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞 全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤:【瘤细胞悬液接种】1)无菌选取生长良好(有光泽,淡红色)瘤组织或对数生长期培养瘤细胞;2)在PBS中将瘤组织剪碎后用匀浆器研磨,经80~100目筛网过滤成细胞悬液;3)培养细胞应用PBS洗两遍;4)计数并调整细胞浓度至107~108/ml;5)常规消毒后,于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位,>106细胞)。初次接种成功率低,细胞数尽可能多一些;6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期,以后随着传代潜伏期逐渐缩短,固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位,引起腹水,腹水中含有瘤细胞 将这种腹水反复传代,即可成为腹水瘤。初次传代时,腹水常呈血性(含大量红细胞),反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤,进行细胞计数;2)消毒动物,左下腹穿刺接种106腹水瘤细胞;3)接种腹水瘤细胞后约7~12d,待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部,用9号针头抽取腹水,也可行腹部解剖后,用滴管吸取。每只小鼠可抽3~5ml;4)抽取的腹水经3000rpm离心15min,收集上清,分装冻存备用。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
6-Chloro-4-(furfurylaMino)pyriMidine, 96%
2-Iodopropane
α-(4-TolyliMino)-p-cresol
(3-ACETYLPHENOXY)ACETIC ACID
Ethyl 2-aMino-4-thiazoleacetate
Monoisopropyl FuMarate , 97%
4,4,4-TRICHLOROACETOACETIC ACID ETHYL ESTER
Gallium(III) oxide (99.998%-Ga) PURATREM
1,3-Phenylene-bis(2-thiourea)
Girard's Reagent P
2-Methyl-1-naphthyl Acetate
ETHYL 2,4-DIMETHYLTHIAZOLE-5-CARBOXYLATE
Cyclobutane-1,2,3,4-tetracarboxylic dianhydride
2-HYDRAZINOBENZOTHIAZOLE
Bis(triMethylsilyl)sulfide
Methanol-d
(-)-(5aS,10bR)-5a,10b-Dihydro-2-(2,4,6-triMethylphenyl)-4H,6H-indeno[2,1-b][1,2,4]triazolo[4,3-d][1,4]oxaziniuM Chloride Monohydrate
5-BroMo-1H-indazole-3-carbaldehyde
4-(2-Methyl-1H-iMidazol-1-yl)benzoic acid hydrochloride hydrate, 95%
TERT-BUTYL ALCOHOL
4-(5-Chloro-2-pyridylazo)-1,3-phenylenediaMine [for ColoriMetric Analysis of Co, Cd]
trichloro-p-tolylsilane
2-Chloro-5-nitrobenzaldehyde
methoxy-bis(dimethylamino)methane
4-Ketopimelic acid
N-(2-BROMOBENZYL)ACETAMIDE
4-Aminotoluene-3-sulfonic acid
N,N-Diphenylbenzidine
Gold(I) sodiuM thiosulfate hydrate, 99.9% (Metals basis)
4-Iodophenol, 98+%
Benzothiophene-2-Methanol
D-(-)-Tartaric acid
3,5-Difluoroanisole
N-t-Butyl 3-broMo-4-MethylbenzenesulfonaMide
2-Boc-AMino-4-chloropyridine
5-TERT-BUTYL-2-METHYLTHIOPHENOL
Montelukast acyl-β-D-glucuronide ,98%
1,3-Benzodioxol-5-ylacetyl chloride 97%
4-Nitrophenyl β-lactoside heptaacetate ,90%
Potassium triethylborohydride, 1M solution in THF, AcroSeal
Ethyl propionylacetate
Sodium 2-amino-1,4-benzenedisulfonate
1-AMINOHEPTADECANE
Thiamine hydrochloride
1-AMinopyrene
5,5'-DibroMo-2,2'-bithiophene
3-Methoxy-4-Methylbenzonitrile
N-(5-Fluoro-2-Methoxyphenyl)acetaMide
Chlorocyclopentadienylbis(triphenylphosphine)rutheniuM(II), 98%
Mebeverine hydrochloride
Hexadecyl Ether
Pigment Red 52:1
1-(6-methylpyridin-3-yl)ethan-1-one
2-(trifluoromethyl)thiophenol
4-Methyl-3-nitropyridine
Heptaldehyde
4-[4-Chloro-3-(trifluoroMethyl)phenyl]-4-piperidinol
POTASSIUM TETRABROMOPLATINATE(II)
3,5-DINITRO-L-TYROSINE SODIUM SALT
Diisopropyl Disulfide
Aminoacetonitrile Hydrogen Sulfate
4-Penten-1-ol
6,7-Dimethoxy-3-methyl-1,2,3,4-tetrahydroisoquinoline hydrochloride, 99%
Valeronitrile
Diethylaminoethanol
N-Methyliminodiacetic acid
Diaminomaleonitrile
3,4-Difluorobenzyl broMide
1-Butyl-4-MethylpyridiniuM BroMide
2-Cyano-1,5-diMethylpyrrole-3-boronic acid, pinacol ester
Chloroacetic acid sodiuM salt, 98%
3,5-Bis(trifluoroMethyl)benzylaMine, 95%
Indanthrene Black BBN
2-Methyl-1,4-pentadiene
2-Azetidinone
4-chloro-7-fluoroquinoline-3-carbonitrile
SERMORELIN ACETATE
( Z )-2-(2-Aminothiazol-4-yl)-2-(t-butoxycarbonylmethoxyimino)acetic acid
CYPA ELISA Kit
PL12/AlaRS ELISA Kit
Human AMELY ELISA Kit
Human dihydropyrimidinase-like 3,DPYSL3 ELISA Kit
AF ELISA Kit
β-Cat ELISA Kit
Chicken IgY (Immunoglobulin Y) ELISA Kit
Human POMT2 ELISA Kit
Human thrombus precursor protein,TpP ELISA Kit
PCDHβ2 ELISA Kit
Ammonium Dextrose Medium
Gelatin Medium
ScandiuM(III) 2,4-pentanedionate hydrate
VanadiuM(III) fluoride, 98%
5-(Boc-aMino)-2-chlorobenzoic acid, 98+%
2-(4-BROMOPHENOXY)TETRAHYDRO-2H-PYRAN
trichloro-p-tolylsilane
2,4-Difluorobenzenesulfonamide
Silver oxide
Phenylacetylene
2,4-Dimethoxybenzeneboronic acid
4-Cyanoindole
Allyltri-n-Butyltin
2,3,4,6-Tetrafluorobenzoyl chloride, 98%
L-3-(FMoc-aMino)-N-trityladipic acid 6-aMide, 95%
PalladiuM(II) trifluoroacetate
α,α-Difluorophenylacetic acid
2,3-DIMETHOXYTOLUENE
4-BroMo-2,5-diMethoxybenzaldehyde
1,2-Decanediol
Methyl 4-thiocyanatobutanoate
Deuterium Oxide BIS001E
2-Cyanoethyltriethoxysilane
Cefpirome Sulfate
Cerium(III) perchlorate hexahydrate, reagent