Rat CEA / Carcinoembryonic Antigen ELISA Kit (Sandwich ELISA) - LS-F34373

Rat CEA / Carcinoembryonic Antigen ELISA Kit (Sandwich ELISA) - LS-F34373
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LS-F34373-1
1 plate
$990 USD
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SpecificationsPublicationsReviewsImagesPopular CEA / Carcinoembryonic Antigen Elisa Kits
Mouse CEA / Carcinoembryonic Antigen ELISA Kit (Sandwich ELISA) - LS-F5042
Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Mouse
Range: 31.25-2000 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Culture Supernatants, Cell Lysates, Serum, Tissue Homogenates, Plasma

Rat CEA / Carcinoembryonic Antigen ELISA Kit (Sandwich ELISA) - LS-F5892
Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Rat
Range: 0.313-20 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Tissue Homogenates, Plasma

Human CEA / Carcinoembryonic Antigen ELISA Kit (Sandwich ELISA) - LS-F5206
Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Human
Range: 19.53-1250 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Culture Supernatants, Cell Lysates, Serum, Tissue Homogenates, Plasma

Human CEA / Carcinoembryonic Antigen ELISA Kit (Sandwich ELISA) - LS-F432
Type: Quantitative Sandwich ELISA
Format: 96-Well Microplate
Reactivity: Human
Range: 0.343-83.33 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Culture Supernatants, Serum, Plasma

Human CEA / Carcinoembryonic Antigen ELISA Kit (Sandwich ELISA) - LS-F9998
Type: Quantitative Sandwich ELISA
Format: 96-Well Microplate
Reactivity: Human
Range: 5-120 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Tissue Homogenates, Plasma

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Product DescriptionLS-F34373 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat CEA / Carcinoembryonic Antigen in samples of Plasma and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of CEA / Carcinoembryonic Antigen as low as 0.094 nanograms per millilter.
About CEA / Carcinoembryonic Antigen
Carcinoembryonic antigen (CEA) describes a set of highly related glycoproteins involved in cell adhesion. CEA is normally produced in gastrointestinal tissue during fetal development, but the production stops before birth. Therefore CEA is usually present only at very low levels in the blood of healthy adults. However, the serum levels are raised in some types of cancer, which means that it can be used as a tumor marker in clinical tests. Serum levels can also be elevated in heavy smokers.
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SpecificationsType
 
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
 
CEA / Carcinoembryonic Antigen
Reactivity
 
Rat
Manual
 

Intended Sample Types
 
Plasma, Tissue Homogenates
Format
 
96-Well Strip Plate
Detection
 
Colorimetric - 450nm (TMB)
Measurement
 
Quantitative
Detection Range
 
0.156 - 10 ng/ml
Sensitivity
 
0.094
Precision
 
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
 
Short term: 4°C; Long term: see manual.
Quality Assurance
 
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
 

• Adhesive Plate Sealers
• Coated 96-well Strip Plate
• Detection Antibody Diluent
• Biotinylated Detection Antibody (100x)
• HRP-Streptavidin Conjugate (100x)
• HRP-Streptavidin Conjugate Diluent
• Sample Diluent
• TMB Substrate
• Stop Solution
• Wash Buffer (25x)
• Standard (Lyophilized)

Background
 
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- CEA antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-CEA antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CEA amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CEA can be calculated.
Restrictions
 
For research use only.
Guarantee
 
This elisa kit carries the LSBio Guarantee.
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