PCK细胞系—大黄鱼肾细胞系全国 货号：GD-C16919716
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息，我们拥有丰富的细胞系培养经验，能提供细胞培养全方位的技术支持，让您实验再无烦扰！公司由在国外工作和学习多年的留学人员创办，由国内ZG科学院上海生物化学与细胞生物学研究所，复旦大学，同济大学等多名工作在线的细胞培养专家，提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念，以世界的技术支撑阵容，提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美，提供了重要的基石；公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品，立足国内，面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位，为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内，在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室，以便更好的服务国内广大医院、大学、制药企业等，你们的每一份关心和支持，都是我们前进的动力！
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
养了几年细胞系—说一点自己培养细胞方面的看法：1)培养细胞前，可以看看细胞特点说明书，包括细胞正常生长的状态图、传代、培养基的类型等；2)不同细胞生长周期不同，有的生长很快，比如说MDA-MB-231，传代的时候取离心悬液的十分之一就已经足够了，有的又是特别慢，等的人心焦，BT474就是，传代是一分二，复苏起始的时候两周多才能长满，所以就要在培养细胞的过程中多多摸索了；3)对于娇弱的细胞系—就得细心呵护了，胰酶消化不能过久，吹得时候要轻柔，离心时候也要注意转速和时间，每天都要去看一下细胞系—肉眼观察培养基状况、显微镜下观察细胞生长状况；4)冻存细胞复苏后第二天Z好更换一次培养基；5)培养箱隔一段时间彻底用酒精棉擦洗一次；6)养细胞的教训：不能偷懒!细胞虐我千百遍，我待细胞如初恋。1)不管买的细胞、惠赠的细胞系—刚拿到手赶紧做两件事：检测是否有支原体污染; 迅速扩增冻存；2)发现有污染迅速处理掉，特别是当你养了很多种细胞时; 细菌污染，真菌污染很容易发现，支原体污染的话，细胞会长的慢，买个支原体检测的kit定期检测一下；3)传代细胞日常的值日清洁是必须的，此外还要定期熏蒸；4)细胞的传代一定要规律，保持相同的confluency和传代时间间隔，不要随心所欲或者拖延；5)注意个人卫生。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
3-BroMobenzoic acid
6-Heptynoic acid
2-AMINO-4-CHLOROBENZOTHIAZOLE HYDROBROMIDE
2-AMINO-6,7-DIMETHYL-4-HYDROXYPTERIDINE
2-Chloro-5-Methoxyaniline hydrochloride
2-BroMo-3-chloropyridine-4-boronic acid
1,2,3,4-Tetrahydroquinoline-6-carboxylic acid
Methyl 3-methoxyacrylate, 95%
Coumarin 337, laser grade, pure, 99%
Di-2-pyridyl thionocarbonate, 97%
2-Iodo-isonicotinic acid methyl ester
trans-2,5-Dimethyl
(2-AMINO-1-PHENYLETHYL)DIMETHYLAMINE
Cadmium fluoride
1-(4-Chlorophenyl)-1-cyclobutanecarboxylic acid
2-BroMo-4-fluoroaniline
(S)-(-)-2,6-DiaMino-4,5,6,7-tetrahydrobenzothiazole
5-AMino-4-cyano-1-(2-chlorophenyl)-3-Methylpyrazole
Tetrahydrothiophen-3-one, 98%
1,2-DICHLOROETHANE-D4
7-Tetradecanol
N,N-diethyloctanamide
2-bromo-5-nitrotoluene
2-AMINO-4-HYDROXY-6-(TRIFLUOROMETHYL)PYRIMIDINE
7-Hydroxy-4-(trifluoromethyl)coumarin
4-HYDROXYBUTYRIC ACID HYDRAZIDE
2-(2-Chloroethoxy)ethanol
2-BroMo-6-Methyl-3-Nitropyridine
2-BroMo-1,3,5-trichlorobenzene, 97%
3-Nonanol, 98%
(7-Azabenzotriazol-1-yloxy)tripyrrolidinophosphoniuM hexafluorophosphate
PIPES MonosodiuM salt
4-BroMo-6-chloro-2H-benzo[d][1,2,3]triazole
N-Ethyl 4-broMo-3-trifluoroMethylbenzenesulfonaMide
2,5-DiMethylpyridine
1,4-DI-N-BUTYLBENZENE
(2-methoxy-3-quinolinyl)boronic acid(SALTDATA: FREE)
1-(Cyclopentyl) 98%
4-Methoxy-2-methylphenyl isocyanate, 98%
5-(Dimethylcarbamoyl)-2-fluorobenzeneboronic acid 98%
1-Acetyl-2-imidazolidinone
2,2'-Bipyridine-4,4'-dicarboxylic acid
2,6-Difluorobenzonitrile
ALPHA-LACTALBUMIN
SodiuM tert-pentoxide
SodiuM phenoxyacetate
alpha,alpha,alpha',alpha'-Tetrafluoro-p-xylene
2-BroMo-4-Methylphenol, 96%
2,4,6-TribroMotoluene, 97%
ELEMI GUM
p-Tolylacetic Acid
3-bromobenzylmagnesium bromide
2-amino-1-pyridin-3-ylethanol
2-Chloro-6-ethoxypyridine
1-(3-bromophenyl)-4,4,4-trifluoro-butane-1,3-dione
Cycloleucine
4-Methoxy-3-trifluoroMethylphenylboronic acid
2-Chloro-4,6-diMethoxypyriMidine
2-ACETOXYACETOPHENONE
3-Fluoro-4-methoxyaniline
3,5-Diiodosalicylic Acid
2,4,6-Trichlorobenzoic Acid
2-(Chloromethyl)-5-methyl-1,3,4-oxadiazole
2-AMINO-4,6-DIMETHOXYPYRIDINE
3-HYDROXY-4-METHOXYPHENETHYLAMINE HYDROCHLORIDE
Benzaldehyde
4-AMINOPYRAZOLO[3,4-D]PYRIMIDINE
Phenolphthalein
1-Octen-3-ol
9-Ethylcarbazole-3-carboxaldehyde Diphenylhydrazone
2-Chloro-5-hydroxybenzonitrile
4-(1H-1,2,4-Triazol-1-yl)benzoic acid, 95%
DIELDRIN
4,4'-Diisocyanato-3,3'-diMethyldiphenylMethane
Pyrimidine, 5-bromo-4-methyl- (7CI,8CI,9CI)
kallikrein from human plasma
5-methylpyrimidine
3,5-Diiodo-DL-tyrosine
Porcine Heat Shock Protein 90,HSP-90 ELISA Kit
Mouse FLI1 (Friend Leukemia Virus Integration 1) ELISA Kit
Human hnRNP K ELISA Kit
Human D4R/DRD4 (Dopamine Receptor D4) ELISA Kit
Monkey αFP (Alpha-Fetoprotein) ELISA Kit
IL12B ELISA Kit
Chicken tumor necrosis factor-related apoptosis-inducing ligand 3,TRAIL-R3 ELISA Kit
Protease ELISA Kit
Human Platelet-Derived Growth Factor-BB,PDGF-BB ELISA Kit
Pax ELISA Kit
Bolton Broth Base
Bolton Broth Base
Manganese(II) Acetate
trans-DiaMMinedichloroplatinuM(II), Pt 64.5% Min
4-n-Butylbenzaldehyde, 97%
Phenethylamine
N,N-diethyloctanamide
thieno[2,3-b]thiophene-2-carboxylic acid
5-Hydroxy-2-methylindole
2,3-dimethylnaphthalene
DIMETHYLTIN DICHLORIDE
Stearyldimethylbenzylammonium chloride
6-Chloro-2-Methyl-3-nitropyridine
5-Hydroxy-2-(trifluoroMethyl)pyridine, 97%
N-Benzyloxycarbonyl-L-alaninol
2,4,6-TriMethylpyridine
3-BroMo-2-hydroxypyridine
3-Hydroxypiperidine
N-BENZYLAMINOACETALDEHYDE DIETHYL ACETAL
0
3-Chloro-2-isobutoxypyridine-5-boronic acid
D-Tetrahydro-furan-2-carboxylic acid
2-Thiophenecarbonyl chloride, 98%
(S,S)-(-)-2,2'-Bis[(R)-(N,N-dimethylamino)(phenyl)methyl]-1,1'-bis[di(3,5-trifluoromethylphenyl) phosphino]ferrocene, min. 97%
2,3-Dimethyl-1H-indol-5-ylamine
