MCG803细胞系—人胃腺癌细胞系全国减价 货号:GD-C16819689
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
培养细胞真的不难,Z关键几点:1)所有的东西使用Z好的,如果你用的血清、培养皿、培养基、胰酶什么的都是进口的,真的很难相信你会把细胞养坏;2)动作要快,胰酶消化,换液什么,细胞暴露在空气中的时间越少越好。另外,要掌握好胰酶消化时间,所谓的细胞状态差,很多时候是传代的原因;3)有时间的话,需要细胞计数,传相应数目的细胞到培养皿中,可以大致清楚细胞的生长曲线,不会临时要处理,手足无措;4)要做什么心里要非常清楚,合理安排时间,传代细胞的实验穿插进行,可以节省很多时间,也不会耽误别人的实验;5)个人的实验器具自己收一套,不要和别人混用,Z近换了什么新的东西稍微记一下,试剂一旦怀疑污染,马上丢;6)传代细胞不能进去太多人,避免相互干扰。每天对待细胞比孝敬爹妈还用心,那真是捧在手里怕碎了,含在嘴里怕化了,看在眼里怕丢了,培养细胞时有哪些经验教训可以分享呢?1)严格无菌操作,多喷喷酒精,要是对灭菌的东西不放心,自己包自己送自己烘干;2)不要和别人共用试剂耗材,自己准备一套;3)有可能的话在拿到新细胞的时候做好支原体衣原体检测;4)水浴锅很脏,生化培养箱要是得手动加湿的话盘里的水也会很脏,勤换(灭菌水加进去);5)晚上离开的时候Z好给传代细胞照紫外,超净台是用完就开;6)Z好的是同一时间就你一个人在用传代细胞在养细胞。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
1-Cyclohexene-1-carboxylic acid
Benzoin diMethyl ether
N-(3-Chloropropyl) 4-boronobenzenesulfonaMide
TrifluoroMethanesulfonic acid tert-butyldiMethylsilyl ester
4-Methyl-3-nitrobenzonitrile
4-ETHYL-1,3-DIOXOLAN-2-ONE
5-Bromo-Indol-3-propionic acid
1-Pyrimidin-2-yl-piperidine-4-carboxylic acid , 97%
(5-Methyl-1-phenyl-1H-pyrazol-4-yl)methanol
2-Methyl-2-propenyl Acetate
2-Cyano-3-nitropyridine
3,5-Dimethylisoxazole-4-boronic acid
Lead arsenite
4-PYRIDYLTHIOUREA
Citraconic anhydride
5,6,7,8-Tetrahydroquinoline
Ethyl Nonafluorobutyl Ether (Mixture of isoMers)
2-AcetaMido-5-chlorophenylboronic acid, pinacol ester
1-(1H-Pyrazol-5-yl)ethan-1-one hydrochloride, 95%
DIBENZ(AE)PYRENE
2,3,3',4,4',5'-Hexahydroxybenzophenone
3,5-dichlorofluorobenzene
1,3-Diphenyl-1,1,3,3-tetramethyldisilazane
diethyl (thiophen-2-ylmethyl)phosphonate
Trans-1,4-cyclohexanedicarboxylic cid
N-BSMOC-L-VALINE
3,4,5-Trimethoxyphenylacetonitrile
Adenosine triphosphate
Glycine diMethylaMide, 97%
Benzhydrazide, 98%
AMantadine hydrochloride
Hexatriacontane
MAGNESIUM METHYL CARBONATE
N,N'-BIS(2-AMINOBENZAL)ETHYLENEDIAMINE
n-Butyl carbaMate
3,3-DiphenylpropylaMine
tert-Butyldimethylsilyl Trifluoromethanesulfonate
1,1,3-Trichloroacetone
2,4,6-Trimethylbenzenesulfonyl hydrazide, 97%
1-Amino-8-naphthol-4-sulfonic Acid
L-ALANYL-L-TYROSINE
DI-N-HEXYLAMINE
Ethacrynic acid
1-Ethyl-3-methylimidazolium tetrafluoroborate
N-PhenylMaleiMide
Phenylphosphonous dichloride
Isooctyl Thioglycolate (Mixture of branched chain isoMers)
2,2-DiMethyl-3-pentanol, 97%
6-[4-(tert-Butoxycarbonyl)piperazin-1-yl]nicotinic acid, 97%
Lincomycin hydrochloride monohydrate
Chlorodiethylisopropylsilane
Aloe barbadensis extract
2,6-Dichloro-1,4-dihydro-3,5-pyridinedicarboxaldehyde
N-Acetyl-L-tyrosine ethyl ester monohydrate (ATEE)
Harpagosid
Trimagnesium dicitrate
3-FLUORO-4-METHOXYBENZYL BROMIDE
2-(4-Chloro-3-nitrobenzoyl)benzoic acid, 98%
STILBAZO
2-Methyl-2-hexanol
Chlorobenzene, pure, 99+%
1-(β-D-Arabinofuranosyl)thymine ,98%
Dimethylvinylmethoxysilane
4-Cyanopyridine
2-CHLORO-6-FLUORO-3-METHYLBENZALDEHYDE
Dimethyl 3,3'-dithiobispropionate
Adenosine 5'-monophosphate monohydrate
BenzyltriMethylaMMoniuM hydroxide
2,2'-DinaphthylaMine
4-Methoxy-2-(trifluoroMethyl)benzaldehyde
(R)-2-(3-Fluorophenyl)pyrrolidine, HCl
[2-(1-PiperidinylMethyl)phenyl]MagnesiuM broMide, 0.25M solution in THF, AcroSeal
2,3-Butanedione monoxime
2,3,4,6-Tetra-O-benzyl-alpha-D-glucopyranosyl Fluoride
2-isobutyl-4-methyl-1,3-dioxolane
6-Fluorosalicylaldehyde
Dichloromethylpentylsilane
2,3-dimethylpyrazine
ES ELISA Kit
Mouse apelin 12,AP12 ELISA Kit
IGFBP3 ELISA Kit
LDL ELISA Kit
MK ELISA Kit
Rat CD3 (Cluster of Differentiation 3) ELISA Kit
AREG ELISA Kit
Human TNKS2 ELISA Kit
IRS-2 ELISA Kit
Col Ⅲ ELISA Kit
Gause’s Synthetic Agar Medium
Folic Acid Assay Medium
Ethyl 1-(hydroxyMethyl)cyclobutanecarboxylate, 97%
Trans-2-Hexenal
4-Ethylpyridine, 98%
Aniline-2-sulfonic acid
3,5-dichlorofluorobenzene
3,5-Dichloro-4-hydroxypyridine
Triphenylmethyl Azide
corynoxeine
Iron phthalocyanine
Cholesteryl oleyl carbonate
4,4-Dibenzoylquinone DioxiMe
2-BroMo-5-(difluoroMethyl)pyridine, 97%
2'-Deoxycytidine hydrochloride, 99%
(2-Chloroethyl)triMethylaMMoniuM chloride, 98% (dry wt.), May cont. up to 5% water
3-Isothioureidopropionic Acid
L-3-Cyanophenylalanine
4-Methoxy-1-butanol
N-Ethyl-4-nitroaniline
4-N-HEXYLOXYBROMOBENZENE
3-(TRIFLUOROMETHYLTHIO)ANILINE
4-(Dimethylamino)butylamine
2'-Deoxyguanosine 5'-monophosphate, sodium salt hydrate, 99%
5-Methyl-3-phenyl-1H-pyrazole