M2e细胞系—人喉癌细胞系全国减价 货号:GD-C16819677
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
培养细胞真的不难,Z关键几点:1)所有的东西使用Z好的,如果你用的血清、培养皿、培养基、胰酶什么的都是进口的,真的很难相信你会把细胞养坏;2)动作要快,胰酶消化,换液什么,细胞暴露在空气中的时间越少越好。另外,要掌握好胰酶消化时间,所谓的细胞状态差,很多时候是传代的原因;3)有时间的话,需要细胞计数,传相应数目的细胞到培养皿中,可以大致清楚细胞的生长曲线,不会临时要处理,手足无措;4)要做什么心里要非常清楚,合理安排时间,传代细胞的实验穿插进行,可以节省很多时间,也不会耽误别人的实验;5)个人的实验器具自己收一套,不要和别人混用,Z近换了什么新的东西稍微记一下,试剂一旦怀疑污染,马上丢;6)传代细胞不能进去太多人,避免相互干扰。每天对待细胞比孝敬爹妈还用心,那真是捧在手里怕碎了,含在嘴里怕化了,看在眼里怕丢了,培养细胞时有哪些经验教训可以分享呢?1)严格无菌操作,多喷喷酒精,要是对灭菌的东西不放心,自己包自己送自己烘干;2)不要和别人共用试剂耗材,自己准备一套;3)有可能的话在拿到新细胞的时候做好支原体衣原体检测;4)水浴锅很脏,生化培养箱要是得手动加湿的话盘里的水也会很脏,勤换(灭菌水加进去);5)晚上离开的时候Z好给传代细胞照紫外,超净台是用完就开;6)Z好的是同一时间就你一个人在用传代细胞在养细胞。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
3-(DiethylaMino)propylaMine
L-glutaMic acid p-nitroanilide
2-BroMo-4'-Methylacetophenone
XyloMetazoline hydrochloride
2-Methoxy-4-Methylpyridine
4,4,4-TRIFLUOROCROTONITRILE
pyrazolo[1,5-a]pyrimidine-3-carbaldehyde(SALTDATA: FREE)
6-Chloro-2-oxindole
boc-gly-osu
2,4-Bis(trifluoromethyl)phenylacetonitrile
5-AMINO-1H-TETRAZOLE
3-Chloro-2-fluoro-pyridine
1-Hydroxybenzotriazole hydrate
3,5-DIBROMOBENZONITRILE
4,4'-Dichlorobenzophenone
4-Methyl-3,4-dihydro-2H-1,4-benzoxazine-7-carbaldehyde
1,2-Bis(MaleiMido)ethane
2-Chloro-4-fluoro-3-Methylbenzonitrile
Methyl 4-iMidazolecarboxylate, 98%
DALAPON
Zopiclone
2-Trifluoromethylphenylboronic acid
Bitumen
1-Tetradecylamine
4-Hydroxybenzamide
3-Octanone
1-(3-DIMETHYLAMINOPROPYL)-3-ETHYLCARBODIIMIDE METHIODIDE
LanthanuM(Iii) Chloride Heptahydrate
N-Propyl-4-[(3S)-pyrrolidinyloxy]benzaMide, 99%
ChroMiuM(III) acetate hydroxide, Cr 24%
4-Nitrobenzenesulfonyl chloride, 97%
Benzyl (S,S,S)-2-azabicyclo[3.3.0]octane-3-carboxylate hydrochloride
4-ForMylfuran-2-boronic acid
N,N'-Di-2-naphthyl-1,4-phenylenediaMine , 96%
2-BROMO-5-NITROTOLUENE
2-(2-(tert-ButyldiMethylsilyloxy)ethyl)phenylboronic acid
4-(3-Methylbutanoyl)phenylboronic acid
Phenylselenocyanate, tech., 90%
(R)-4-Formyl-2,2-dimethyl-oxazolidine-3-carboxylicacid tert-butyl ester
2-(chloromethyl)-4-methoxypyridine(SALTDATA: 1.02HCl 0.19H2O)
5-Methyl-2-nitrobenzaldehyde
Boc-L-Glutamic acid
Phosmet
BENZOYL BROMIDE
trans-Ethyl cinnaMate
2-BroMo-1-(1-Methyl-1H-benziMidazol-2-yl)-1-ethanone
(1R,2R)-N,N'-Dihydroxy-N,N'-bis(diphenylacetyl)cyclohexane-1,2-diaMine
2,4-DiMethylaniline-5-sulfonic Acid SodiuM Salt Hydrate
Undecylbenzene, 99%
SodiuM Hypochlorite Solution, 5 Percent Available Chlorine, 5 Percent Available Chlorine, Solution, Reagent, ACS
4-(TrifluoroMethoxy)phenol
Diethyl fumarate
2-Chloro-1-phenyl-1-propanone
4-Chlorostyrene
4-fluoro-3-iodotoluene
MAGNESIUM HYDROXIDE
Ethyl butyrylacetate
TERT-BUTYLCARBAMIDINE HYDROCHLORIDE
PotassiuM bis(triMethylsilyl)aMide
[2-(3-Cyclohexenyl)ethyl]trichlorosilane
tetrazolo[1,5-a]pyridine-6-carboxylic acid(SALTDATA: FREE)
cis-1-N-Boc-4-Methoxy-L-proline
Methyl 5-chloropentanoate, 97%
Gabapentin-lactam
3-Amino-2-pyridinecarbonitrile
1-TRIDECYNE
3-Amino-3-(4-fluorophenyl)propanoic acid
1-Methyl-4-(3-nitrophenyl)
2,2'-Carbonylbis(3,5-dioxo-4-Methyl-1,2,4-oxadiazolidine)
4-(2-Nitrophenoxy)phenylboronic acid
Glyoxylic acid Monohydrate, 98%, pure
CalciuM citrate tribasic tetrahydrate, 98+%, for analysis
8-AMino-1-naphthalenesulfonic Acid
Glycerol [Matrix for FABMS and liquid SIMS]
Ethylmagnesium Chloride
2,4-Dichlorobenzylamine
(R)-1-(3-Bromophenyl)ethylamine
Ethyl 2-(2-mercapto-4-methyl-1,3-thiazol-5-yl)acetate
NOX4 ELISA Kit
BAX ELISA Kit
Human IGFBP-5 ELISA Kit
LRP1 ELISA Kit
TNFsR-I ELISA Kit
LTC4 ELISA Kit
NE ELISA Kit
TE ELISA Kit
Human INSR (Insulin Receptor) ELISA Kit
Mouse Collagen Type Ⅱ,Col Ⅱ ELISA Kit
mEI Agar
Nutrient Glucose Agar(Waksman, 1957)
Ethyl 2-(2-thienyl)thiazole-4-carboxylate, 97%
trans-3-(TrifluoroMethyl)cinnaMoyl chloride, 99%
4-EthylMorpholine, 98%
2-AMinobenzaMide, 98+%
2-Trifluoromethylphenylboronic acid
3-Cyanocinnamic acid
Xipamide
Hexatriacontane
THULIUM 2,4-PENTANEDIONATE, TRIHYDRATE
6-AMINO-2-NAPHTHOIC ACID
1,3-Di-tert-butylbenzene, 99%
4-Fluoro-3-(trifluoroMethyl)aniline, 98%
N-Benzyl-N-phenyl-4-fluorobenzaMide, 97%
Folinic acid calciuM salt hydrate
TETRONIC ACID
AluMiniuM sulfate
3-METHOXY-5-METHYLPHENOL
1,3-Bis(aMinoMethyl)benzene
5-BroMo-4-p-tolylpyriMidine
3-(Perfluoro-3-methylbutyl)-1,2-propenoxide
5-Amino-2-pyridinol hydrochloride
(S)-1,2-Diaminopropane Dihydrochloride
p-(t-Butyl)phenethyltrichlorosilanecontains ~ 5% meta isomer