EL-4细胞系—小鼠淋巴瘤细胞系全国减价 货号:GD-C16819582
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
培养细胞真的不难,Z关键几点:1)所有的东西使用Z好的,如果你用的血清、培养皿、培养基、胰酶什么的都是进口的,真的很难相信你会把细胞养坏;2)动作要快,胰酶消化,换液什么,细胞暴露在空气中的时间越少越好。另外,要掌握好胰酶消化时间,所谓的细胞状态差,很多时候是传代的原因;3)有时间的话,需要细胞计数,传相应数目的细胞到培养皿中,可以大致清楚细胞的生长曲线,不会临时要处理,手足无措;4)要做什么心里要非常清楚,合理安排时间,传代细胞的实验穿插进行,可以节省很多时间,也不会耽误别人的实验;5)个人的实验器具自己收一套,不要和别人混用,Z近换了什么新的东西稍微记一下,试剂一旦怀疑污染,马上丢;6)传代细胞不能进去太多人,避免相互干扰。每天对待细胞比孝敬爹妈还用心,那真是捧在手里怕碎了,含在嘴里怕化了,看在眼里怕丢了,培养细胞时有哪些经验教训可以分享呢?1)严格无菌操作,多喷喷酒精,要是对灭菌的东西不放心,自己包自己送自己烘干;2)不要和别人共用试剂耗材,自己准备一套;3)有可能的话在拿到新细胞的时候做好支原体衣原体检测;4)水浴锅很脏,生化培养箱要是得手动加湿的话盘里的水也会很脏,勤换(灭菌水加进去);5)晚上离开的时候Z好给传代细胞照紫外,超净台是用完就开;6)Z好的是同一时间就你一个人在用传代细胞在养细胞。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
5-BroMo-1-indanone
(R)-(+)-2-(DiphenylMethyl)pyrrolidine
4,4'-Methylenebis(2-ethyl-6-Methylaniline)
3-(2-BENZOTHIAZOLYLTHIO)PROPIONIC ACID
EthylaMine hydrochloride
N-SucciniMidyl 4,4,5,5,6,6,7,7,8,8,9,9,10,10,11,11,11-heptadecafluoroundecanoate
Phenylglyoxal monohydrate, 97%
1,4-Epoxy-1,4-dihydronaphthalene
5-Methyl-pyrazine-2-carboxamide
Tetrabutylammonium Bifluoride
3-FLUORO-4-METHOXYPHENYLACETIC ACID
3-FLUORO-4-(TRIFLUOROMETHYL)BENZYL BROMIDE
2,5-Dimethylphenylacetonitrile
2-Aminoethylmethylsulfone hydrochloride
L-GlutaMic acid γ-Benzyl ester
1-Methyl-1H-iMidazole-5-carbonitrile
4-Ethoxy-2-butanone
2-Hydroxy-4-pyridinecarboxaldehyde
2,5-Difluorobenzaldehyde, 98%
ACIFLUORFEN
8-Chloroquinoline
N,N,N-trimethyl-1-dodecanaminium bromide
Cis-Hexahydrophthalimide
Azidoacetic acid methyl ester
2,3-Dimethylbenzoic acid
1,3-Dioxolane
BENZOYL ISOCYANATE
3-AMino-5,6-diMethyl-1,2,4-triazine
3-Chloro-2-fluoropyridine, 95%
2,2'-Dichlorobenzophenone, 98+%
FMoc-Pro-OH
CadMiuM arsenide
Tris(isopropylcyclopentadienyl)praseodyMiuM(III), 99.9% (REO)
N-AMYL NITRATE
(S)-RuCl[(p-cyMene)(DTBM-SEGPHOS?)]Cl
2,4-Dichlorophenoxyacetic acid
1-Undecene [Standard Material]
4-(hydroxymethyl)-4-piperidinol(SALTDATA: HCl)
(2-Pyrrolidin-1-ylpyrid-4-yl)methylamine, 97%
4-Methyl-3-(piperidine-1-sulfonyl)benzoic acid
Dipotassium hexachloroiridate
Ethyl 2-methylbenzoate
Isophytol
GUANOSINE 5'-DIPHOSPHATE SODIUM SALT
5-Nitrothiophene-3-carbonitrile
Crotaline
4-Methoxyphenylphosphonic Dichloride
4-[3-(TrifluoroMethyl)phenyl]phenylacetic acid
2-AMino-5-broMopyridine, 97%
NICKEL TETRAFLUOROBORATE
Ethyl 2-Fluoropropionate
Zinc carbonate
(1-hydroxyethylidene)bisphosphonic acid, sodium salt
ecteola cellulose fine mesh
3,4-(Methylenedioxy)phenylacetonitrile
Holmium(III) nitrate pentahydrate
1,3-DibroMo-5-fluoro-2-Methoxybenzene
Penicillin G PotassiuM Salt
2-Chlorobutyryl chloride, tech. 85%
4-Amino-2-methylthio-quinazoline
N-Decanoyl-D-erythro-sphingosine, synthetical, 99+%
1-(4-Nitrophenyl)pyrrolidine
Isobutyl Nitrite
3-Methyl-5-nitro-1H-indazole
2-HYDROXY-6-(TRIFLUOROMETHYL)PYRIDINE
Tecloftalam
Dimethyl sulfate
1,1-Cyclohexanediacetic acid
2-(EthylaMino)ethanol
4,4-Difluorocyclohexanecarboxylic Acid
2'-AMinobiphenyl-3-carboxylic acid
DiMethyl nitroterephthalate, 99%
1,2,4,5-TETRACHLOROBENZENE
5-Nitroguaiacol
7,7-dichlorobicyclo[4.1.0]heptane
4,4'-sulfonylbis(2-nitrophenol)
cholesteryl hemisuccinate free acid
Benzophenone oxime
GOD ELISA Kit
CD45 ELISA Kit
Human Pancreastatin ELISA Kit
Human apoptosis signal regulating kinase 1,ASK-1 ELISA Kit
MAPT ELISA Kit
Rat soluble interleukin-2 receptor,IL-2sRα ELISA Kit
IGFBP-3 ELISA Kit
Human APLP2 ELISA Kit
Human OCLM ELISA Kit
HMGCS1 ELISA Kit
MacConkey Agar
Rappaport-Vassiliadis R10 broth
ETHYLHYDROCUPREINE HYDROCHLORIDE
Trans-1,4-CyclohexanediMethanol
4-Ethoxy-2-fluoroaniline, 97%
2-AMinoisobutyric acid Methyl ester hydrochloride, 99%
N,N,N-trimethyl-1-dodecanaminium bromide
1H-Benzimidazole-5-carboxylic acid
N-tert-Butoxycarbonyl-L-glutamic acid gamma-tert-butyl ester
benzenesulphinic acid
2,3,4-TRIFLUOROPHENOL
1-Bromo-4-chlorobutane
2-BroMo-3-fluorobenzaldehyde
Tris(2,2,6,6-tetraMethyl-3,5-heptanedionato)praseodyMiuM(III), 99%
1,4-Dioxene, 98%
Saikosaponin D froM BupleuruM falcatnuM
L-Methionine
N-Boc-6-broMohexylaMine
EthanaMine, 2-(2-fluorophenoxy)- (9CI)
4-BroMo-2,6-difluoroanisole
1-[(4-Methylphenyl)sulfonyl]-1H-iMidazole
H-Lys-OMe.2HCl
5-(5-Bromo-2-thienyl)isoxazole, 97%
Perfluoro(1,2-oxathietane 2,2-dioxide)
2-Methyl-3-(trifluoromethyl)benzyl bromide