SW962[SW 962,SW-962]细胞系—人阴户磷癌细胞系全国减价

SW962[SW 962,SW-962]细胞系—人阴户磷癌细胞系全国减价 货号：GD-C16820381
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息，我们拥有丰富的细胞系培养经验，能提供细胞培养全方位的技术支持，让您实验再无烦扰！公司由在国外工作和学习多年的留学人员创办，由国内ZG科学院上海生物化学与细胞生物学研究所，复旦大学，同济大学等多名工作在线的细胞培养专家，提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念，以世界的技术支撑阵容，提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美，提供了重要的基石；公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品，立足国内，面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位，为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内，在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室，以便更好的服务国内广大医院、大学、制药企业等，你们的每一份关心和支持，都是我们前进的动力！
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤：【瘤细胞悬液接种】1)无菌选取生长良好(有光泽，淡红色)瘤组织或对数生长期培养瘤细胞；2)在PBS中将瘤组织剪碎后用匀浆器研磨，经80～100目筛网过滤成细胞悬液；3)培养细胞应用PBS洗两遍；4)计数并调整细胞浓度至107～108／ml；5)常规消毒后，于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml／部位，＞106细胞)。初次接种成功率低，细胞数尽可能多一些；6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期，以后随着传代潜伏期逐渐缩短，固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位，引起腹水，腹水中含有瘤细胞系—将这种腹水反复传代，即可成为腹水瘤。初次传代时，腹水常呈血性(含大量红细胞)，反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤，进行细胞计数；2)消毒动物，左下腹穿刺接种106腹水瘤细胞；3)接种腹水瘤细胞后约7~12d，待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部，用9号针头抽取腹水，也可行腹部解剖后，用滴管吸取。每只小鼠可抽3~5ml；4)抽取的腹水经3000rpm离心15min，收集上清，分装冻存备用。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
5-Nitro-M-xylene, 99%
Arg-Phe acetate salt
2-AMino-4,5-diMethoxybenzonitrile
1,6-Diisocyanohexane
2-N-PROPYLTHIAZOLE
ALLYL N-PROPYL SULFIDE
4-METHYL-2-PYRROLECARBOXYLIC ACID ETHYL ESTER
6-Iodo-pyridine-2-carboxylic acid methyl ester
4-(Hydroxymethyl)cyclohexanecarboxylic Acid (cis- and trans- mixture)
Ethyl 1,2,3-thiadiazole-5-carboxylate
Ketoconazole
2-Chlorobenzene-1,4-diammonium sulphate
5-FLUORO-4-HYDROXYQUINAZOLINE
2-CHLOROBENZENESULFONYL ISOCYANATE
2-BroMo-5-nitropyridine
Silver nitrate
(1R,2R)-N,N'-DiMethyl-N,N'-bis(3,3-diMethylbutyl)cyclohexane-1,2-diaMine
Methyl 5-broMo-4-chloro-8-Methylquinoline-2-carboxylate
VitaMin D2, 99%
SodiuM Thiosulfate, Pentahydrate, Reagent, ACS
Dihydrogen Di-Mu-chlorotetrakis(di-tert-butylphosphinito)dipalladate
Valeryl chloride
N-chloroacetylglycine
4-(2-pyridylazo)resorcinol
4-amino-3-fluorobenzoic acid
(R)-(-)-2-AMINO-6-PHOSPHONOHEXANOIC ACID HYDRATE
Methyl Green zinc chloride salt
D-Alaninol
2-BroMo-5-fluorobenzyl broMide, 97%
2-Chloro-2,2-difluoroacetaMide, 98%
1-(tert-ButyldiMethylsilyl)indole-5-boronic acid, 97%
3-(Boc-aMinoMethyl)azetidine hydrochloride
DiMethyl 3,3'-dithiobispropionate
PalladiuM(II)-aMMoniuM chloride
Chlorodicarbonyl(1,2,3,4,5-pentaphenylcyclopentadienyl)rutheniuM(II)
4-DIMETHYLAMINO-1-NAPHTHYL ISOTHIOCYANATE
1-(3-Methoxyphenyl)-1-heptanone
Calcium phosphate, dibasic (99.95%-Ca)
Acid Blue 20
3-Bromo-N-butylbenzenesulphonamide 98%
2,6-Dichloro-4-(piperidin-1-ylcarbonyl)pyridine
2-Picoline
1-Bromo-3,4,5-trimethoxybenzene
Calcium hydrogenphosphate dihydrate
Benzamidine hydrochloride
1-BroMo-2,6-dichlorobenzene
6-DiMethylaMino-1-hexanol
3-BroMo-4-Methoxyphenyl Mesylate
PotassiuM broMate, ACS reagent
Fullers Earth, Powder
2-PhenylethylaMine
n-Butyl Benzoate
Direct Yellow 4
Anethole Trithione
2,3-Dichloro-1-propene
2,6-Difluorobenzoic acid
3'-AMino-4'-Methoxyacetanilide
1-(6-CHLOROPYRIDAZIN-3-YL)PIPERIDINE-4-CARBOXAMIDE
Luteolin
4-Fluorobenzophenone
5-Bromo-2-methoxycinnamic acid
Lead antimonide, 99+%
5-Chloro-3-methyl-1-p-tolyl-1H-pyrazole-4-carbaldehyde
5-Aminonicotinic acid
C13-C16 Isoparaffin
BOC-D-GLU-OH
4-Nitrophenyl-beta-D-galactopyranoside
tert-butyl 4-[4-(4,4,5,5-tetraMethyl-1,3,2-dioxaborolan-2-yl) phenyl]-1-carboxylate
1-BroMo-2-fluoroethane
2,2,3,4,4,4-Hexafluoro-1-butanol
5-Fluoro-2-Mercaptobenzothiazole
2,5-DiMethyl-1,3-oxazole-4-carbonyl chloride, 97%
Z-VAL-ALA-OME
3-Mercaptopropionic Acid
m-methylcinnamic acid
LEAD SALICYLATE
1-phenylpiperidine
tylosin
CML ELISA Kit
Mouse Slit2 ELISA Kit
FBN1 ELISA Kit
Human ubiquitin D,UBD ELISA Kit
Chicken NT-ProBNP (N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
a-Glu ELISA Kit
Chicken very low density lipoprotein,VLDL ELISA Kit
PC/CPG ELISA Kit
HO2 ELISA Kit
IGFBP1 ELISA Kit
Wheat Bran Medium
Strain Medium
Ethyl acetoacetate
Zinc fluoride tetrahydrate, 98%
5,6-DiMethoxy-1-indanone, 97%
2-Fluoro-4-hydroxybenzeneboronic acid pinacol ester, 96%
Valeryl chloride
Ammonium iron(III) oxalate hydrate
Methamidophos E.C.
Benzenediazonium, 3-bromo-, tetrafluoroborate(1-)

Guaiacol carbonate
TRIPHENYLMETHYL MERCAPTAN
2-Propanethiol
Iron(III) fluoride trihydrate
(3-Benzyloxypropyl)triphenylphosphoniuM broMide, 98%
2-BroMo-6-fluorobenzonitrile
StrontiuM nitrate
4-(N,N-DiMethylsulfaMoyl)-2-Methylphenylboronic acid
5-Butoxy-4-chloro-2-fluorophenylboronic acid, pinacol ester
3,4-DIHYDRO-2H-1,5-BENZODIOXEPINE-7-CARBOXYLIC ACID
4-tert-Butylphthalic anhydride
Berberine Sulfate Hydrate
5-Bromo-4-methylpyrimidine
Pyrazolo[1,5-a]pyridine-3-carboxylic acid , 97%
Dilithium tetrabromonickelate(II), 0.5M solution in THF, AcroSeal