293 Cells,low passage细胞系—人胚肾细胞系全国减价

293 Cells,low passage细胞系—人胚肾细胞系全国减价 货号：GD-C16819227

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息，我们拥有丰富的细胞系培养经验，能提供细胞培养全方位的技术支持，让您实验再无烦扰！公司由在国外工作和学习多年的留学人员创办，由国内ZG科学院上海生物化学与细胞生物学研究所，复旦大学，同济大学等多名工作在线的细胞培养专家，提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念，以世界的技术支撑阵容，提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美，提供了重要的基石；公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品，立足国内，面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位，为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内，在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室，以便更好的服务国内广大医院、大学、制药企业等，你们的每一份关心和支持，都是我们前进的动力！
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，原因比较复杂，常见原因可归纳为：培养基使用错误或培养基品质不佳；血清使用错误或血清的品质不佳；解冻过程错误；冷冻细胞解冻后，加以洗涤细胞和离心；悬浑细胞误认为死细胞；培养温度使用错误；细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来，其离心速率应为多少转速?欲回收动物细胞系—其离心速率一般为300×g(约1OOOrpm),5-10分钟，转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2，其中r为离心机转轴ZX与离心套管底部内壁的距离；rpm为离心机每分钟的转数；RCF(relative centrifugal force)为相对离心力，以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
BisMuth subgallate hydrate
Bulleyaconitine A
2-(3-Benzoylphenyl)propionitrile
Asiatic acid
0
3-(TrifluoroMethyl)benzaldehyde
1,1′-Bis[(2R,5R)-2,5-diisopropylphospholano]ferrocene
2-(Methylthio)nicotinic acid, 97%
cis-2-Methylcyclohexanol
Dansylglycine
6-O-(Triisopropylsilyl)-D-galactal
(S)-(+)-4-Phenyl-2-oxazolidinone
1-METHYLTHIO-2-PROPANONE
Nafamostat mesylate
5-AMino-2-chloropyridine
3,6-Dichloropyridazine
1,3-Dihydro-1-Methoxyisobenzofuran
Methyl 2-broMo-4,5-difluorobenzoate
Xylitol, 99+%
Sorbitan Sesquioleate, NF
N-Acetyl-2,3-didehydro-2-deoxyneuraMinic Acid
tricosane
D-(-)-Gulonic acid gamma-lactone
3,5-diphenyl-1H-pyrazole
4-Fluorobenzamidine hydrochloride
POLYPHENYLMETHYLDIMETHYLSILOXANE
Guanosine-5'-diphosphate disodium salt
Ethyl ethoxyacetate
EthylenediaMinetetraacetic acid, iron(III) MonosodiuM salt
1,3-Diphenylisobenzofuran, 97%
1,2:5,6-Di-O-isopropylidene-α-D-allofuranose
ChloroMethyl chlorosulfate
ERYTHROSIN B
Oxindole-4-boronic acid, pinacol ester
2-BroMo-6-Methoxybenzaldehyde
4-AcetaMido-1-(3-chloro-2-pyridyl)piperidine, 96%
Poly(benzyl methacrylate), average M.W. 70000
Duroquinone, 97%
Potassium (4-methoxy)benzyloxymethyltrifluoroborate
Methyl 2-methyl-3-furancarboxylate, 97%
Ethyl 4-hydroxyphenylacetate
4-tert-Amylphenol
Ferric ammonium oxalate trihydrate
Hexyl acetate;n-Hexyl acetate
4-Methoxy-alpha-toluenethiol
5-CouMarancarboxaldehyde
4-Nitrotoluene-2-sulfonic Acid Hydrate
D-GlutaMic acid alpha-aMide, HCl
PalladiuM(II) nitrate hydrate
Cineole
1-EthylpyridiniuM Chloride
ethyl 4-chloro-1,3-dimethyl-1H-pyrazolo[3,4-b]pyridine-5-car...
1-Amino-3,3-diethoxypropane
3-METHOXYPYRIDINE 1-OXIDE
barium diethyl bis(sulphate)
2-Bromo-4'-methoxyacetophenone
Ethylene glycol
Cytidine 5'-Monophosphate DisodiuM Salt Hydrate, 99%
2-FLUORO-6-METHOXYBENZOIC ACID
4'-Hydroxyhexanophenone
2,2-Difluorosuccinic acid
1,2-Dibromohexane, 97+%
3-Iodo-4-methoxy-1H-pyrrolo[2,3-b]pyridine
Methyl 2-benzoylbenzoate
5-Chloro-2-methoxyaniline
Fmoc-O-benzyl-L-tyrosine
2-Allyloxyethanol
4-MethyluMbelliferyl-beta-D-glucopyranoside
1-BroMo-3-(triMethylsilyl)benzene
4-(1,3-Dioxolan-2-yl)-2,6-difluorophenylboronic acid
D-Cysteine, 99%
{2-[1-(tert-Butoxycarbonyl)piperidin-4-yl]-5-Methyl-1,3-thiazol-4-yl}acetic acid, 97%
1,1'-SulfonyldiiMidazole
PentafluorobenzaMide
3-Acetylthianaphthene
2-Methoxy-4-(piperazin-1-yl)phenol
Boc-D-pyroglutaminol
2,3-Dihydroxypyridine
MPO-ANCA IgG ELISA Kit
Salusin a ELISA Kit
Human FBN1 (Fibrillin 1) ELISA Kit
Human UBA52 (Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1) ELISA Kit
β-lactamase ELISA Kit
α-SYNo ELISA Kit
T4 ELISA Kit
CHE ELISA Kit
Human alpha-granular membrane protein,GMP-140 ELISA Kit
Amylin ELISA Kit
Halophilic Bacteria Selective Agar
Eugon LT 100 Agar Base
(4-Nitrophenyl)Methyl 3-oxobutanoate
AntiMony trifluoride
5,6-Diphenyl-2-Morpholinone, 98%
2-Fluoro-4-Methylpyridine-5-Boronic Acid
tricosane
2-pentenenitrile
Lanthanum(III) nitrate hexahydrate
Kojic acid

(1S,4R)-2-AZABICYCLO[2.2.1]HEPTAN-3-ONE
ETHIOFENCARB-SULFOXIDE
2,6-Dihydroxyacetophenone
5-Methyl-2-(3-nitrophenyl)-3-pyrazolinone, 98%
(S)-(-)-N-(3-Pentyl)-1-phenylethylaMine hydrochloride, 99%
Hexanophenone, 98%
2-Fluorotoluene
D-Leucinol
2-(N-Boc)-5-(tributylstannyl)thiazole
5-CHLORO-3-METHYL-1-PHENYL-1H-PYRAZOLE-4-CARBONITRILE
2-FLUORO-5-METHOXYBENZALDEHYDE
2-AMino-3-chloro-5-trifluoroMethylpyridine
Diethyl dimethylmalonate, 97%
n-Octanohydrazide
Phthalimidoacetone