HBL-100[HBL100]细胞系—人整合SV40基因的乳腺上皮细胞系全国减价 货号:GD-C16819167
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞系—其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
4-Chlorobenzotrifluoride, 98+%
JASMIN CONCRETE FINE
N-Ethyl 3-broMo-5-trifluoroMethylbenzenesulfonaMide
5-BroMo-2-ethoxyphenylboronic acid
2-(4-Methylcyclohexylidene)acetic acid
Ethyl 4-Methyl-2-MorpholinopyriMidine-5-carboxylate
ETHYL 3-METHYL-4-NITROBENZOATE
4-Acetamidobenzoic Acid
2-N-BOC-Amino-3-(4-tetrahydrothiopyranyl)propionic acid, 95%
4-Bromo-2-fluorobenzylamine, 99%
1-Bromo-4-(tert-butylsulfanyl)benzene
1,2,3,4-TETRAHYDRO-ISOQUINOLIN-6-OL
Lithium diisopropylamide
2-MERCAPTO-6-NITROBENZOTHIAZOLE
LissaMine Green B
3-BroMo-2-Methylpyridine
DiMethyl cis-1,2-Cyclohexanedicarboxylate
Voglibose
Tungsten hexacarbonyl, 99%
SodiuM Carbonate, Anhydrous, Technical
3-Cyano-2-fluoropyridine
3-(dimethylamino)-1,2-propanediol
Ethyl diethoxyacetate
2-Bromofluorobenzene
6-fluoroquinoline
POTASSIUM CARBONATE, SESQUIHYDRATE
5-Bromouridine
4-Iodoaniline
2-Chloro-3-fluoropyridine, 97%
1-Heptene, 98+%
EthylenediaMinetetraacetic acid tetrasodiuM salt
Methyl 4-(cyanoMethyl)benzoate
4'-DIETHYLAMINOACETOPHENONE
PetroleuM ether 60/80
N-t-Butyl 2-(5-broMopyridin-2-yl)acetaMide
2-(4-BroMobenzaMido)ethyl 4-broMobenzoate
Propionaldehyde Dimethyl Acetal
Triphenylstannyl butyl dimethyl silane
4,4,5,5-Tetramethyl-2-(3-trifluoromethylphenyl)-1,3,2-dioxaborolane
5-(cyclohexylthio)-2-furaldehyde(SALTDATA: FREE)
Palladium diacetate
6-Hydroxy-1-indanone
NICKEL POTASSIUM CYANIDE
Methyl vanillate;Methyl 4-hydroxy-3-Methoxybenzoate
2-Pentanone
2,5-Lutidine
Penicillin G SodiuM Salt
1,1'-Bis(diphenylphosphino)ferrocene, 98%
5-(BroMoMethyl)-1-Methyl-1H-1,2,3-benzotriazole, 95%
Mitoxantrone hydrochloride
1,2-Epoxypentane
3,4-Dicyano Aniline
10,12-Tricosadiynoic acid
Triisooctyl phosphite
4-Benzyloxy-3-nitroacetophenone
3,4-DINITROANILINE
N-[α-(4-Methylphenyl)sulfonyl)benzyl]diphenylphosphinic aMide
D-(-)-ALPHA-PHENYLGLYCINE ETHYL ESTER HYDROCHLORIDE
1-BENZYL-4-(ETHOXYCARBONYLMETHYL)
3-(Cyclopentyloxy)-4-methoxybenzoic acid
2-Chloro-5-fluorobenzyl alcohol
3-Iodo-o-xylene
tert-Butyl (2-aminophenyl)carbamate
3,4-Epoxytetrahydrofuran
2,4-Dichloro-5-nitrophenol
Tetrachloropropene
3-Fluorophenol
2',5'-Dichloroacetophenone
1-Benzyl N-(tert-Butoxycarbonyl)-L-glutaMate
4'-Ethylbiphenyl-3-carboxylic acid
Benzoyl broMide, 97%
N-(tert-Butoxycarbonyl)-L-alanine N'-Methoxy-N'-MethylaMide, 98%
1-(M-Tolyl)
2',4'-Difluoroacetophenone
2,3,5,6-Tetrafluorobenzoic acid
N-Boc-3-piperidinepropionic acid
sodium phenothiazine-10-yl-propylsulfonate
1-bromo-7-chloroheptane
RANTES ELISA Kit
TLR3 ELISA Kit
Human apoprotein A5,apo-A5 ELISA Kit
Human AhR ELISA Kit
Chicken Interleukin 4,IL-4 ELISA Kit
α1AGP ELISA Kit
Chicken toxoplasma circulating antigen,TCA ELISA Kit
Human monocyte chemotactic protein 2,MCP-2 ELISA Kit
ADAMTS13/vWF-cp ELISA Kit
PDGF ELISA Kit
Tryptose Phosphate Broth
Ureaplasma urealyticum Agar Base
ZirconiuM(IV) isopropoxide isopropanol coMplex
FERBAM
5-(Phenylmethoxy)-1H-indole-3-carbaldehyde
2-FLUORO-5-HYDROXYBENZALDEHYDE
3-(dimethylamino)-1,2-propanediol
4-fluoro-2-iodotoluene
BES
4'-fluoropropiophenone
Thulium oxide
4'-(HEXYLOXY)-4-BIPHENYLCARBONITRILE
3-Chloro-5-Hydroxy-Benzonitrile
5-BroMo-2-(tert-butoxy)pyriMidine, 95%
6-Fluoroquinoline, 97%
3-Methyl-3-(p-toluenesulfonyloxyMethyl)oxetane, 98%
FenaMiphos
N1,N5-Bis-Boc-sperMidine
2-Chloro-3-(pyrrolidin-1-ylsulfonyl)pyridine
3-Chloro-4-Methylpyridine
AMinoguanidiniuM nitrate
7-METHOXY-3-PHENYLSULFONYL-1(3H)-ISOBENZOFURANONE
4-Fluoro-1H-indazole
Ancymidol
Phenyl Isonicotinate