CAL-62细胞系—人甲状腺癌细胞系全国减价 货号:GD-C16819084
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
常见细胞培养中一些问题总结:1)培养基中血清的比例多少合适?血清是细胞培养上一个极为重要的营养来源,所以血清的含量多少会对细胞的生长会产生极大的影响。血清的含量一般为5%-15%,不要低于5%或高过20%,因为含量过低会导致细胞营养不足,过高则会破坏渗透压平衡。一般建议血清含量为10%,可以适量加减;2)何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上的更换时间,按时更换培养基即可。还可以参照指示剂的颜色变化进行更换;3)购买的复苏细胞系—为何会有脱落?因为运输的过程中不可避免的会有震荡,收到后可以离心收集;4)购买的细胞冷冻管经解冻后,为何会发生细胞数目太少之情形?研究人员在冷冻细胞之培养时出现细胞数目太少,大都是因为离心过程操作上的失误,造成细胞的物理性损伤,以及细胞流失。建议细胞解冻后不要立刻离心,应待细胞生长隔夜后再更换培养基即可;5)购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,常见原因可归纳为:培养基使用错误或培养基品质不佳。血清使用错误或血清的品质不佳。解冻过程错误。冷冻细胞解冻后,加以洗涤细胞和离心。悬浮细胞误认为死细胞。培养温度使用错误。细胞置于–80°C太久;6)收到的冷冻管瓶身破裂,瓶盖有裂纹,或瓶盖脱落之原因?冷冻管瓶盖裂纹,或瓶身破裂,可能是因为操作者夹取冷冻管时用力不当,造成冷冻管裂损,建议使用止血钳小心夹取。另冷冻管瓶盖松动或松脱,乃因热胀冷缩之物理现象,冷冻管有可能因此而造成细胞污染,故冷冻管于放入和取出液氮桶时,均应立刻将冷冻管再一次扭紧。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
N-Acetyl-L-alanine, 96%
4-Chlorophenethyl broMide
2-METHOXYBENZOPHENONE
DL-1-Phenethylalcohol
4,5-Difluoro-2-Methoxyphenylboronic acid
3-(DICYANOMETHYLIDENE)INDAN-1-ONE
DIMETHYL BROMOMALONATE
Methyl 3-bromothiophene-2-carboxylate , 97%
2-Fluoropyridine-4-boronic acid, pinacol ester
[2-(4-Methoxy-phenyl)-5-oxo-cyclopent-1-enyl]-acetic acid
2,3-Difluorophenylisothiocyanate
4-Bromo-o-xylene
N-ACETYL-DL-NORLEUCINE
Disodium citrate
PhosphoMolybdic acid hydrate
1-Phenyl-2-thiourea
1,3-Di-O-benzyl-2-O-lauroylglycerol
N-Isopropyl 2-broMo-5-fluorobenzaMide
TriMethylsilylacetylene, 98%
PyBOP
N,N'-Bis(diMethylaMinoMethylene)hydrazine
5-Nitrouracil
3-(CYANOMETHYL)BENZOIC ACID
4,5-dichlorophthalic anhydride
1,1,1-trifluoro-2-butanone
(HEPTAFLUOROBUTYRO)PHENONE
Ethyl gallate
PhenylMagnesiuM broMide, 3M in 2-MeTHF
N-Boc-D-valine, 98+%
2-Mesitylethanol, 98%
2,2,2-Trichloroethanol
1-METHOXY-4-(METHYLTHIO)BENZENE
N-(4-AMinobutyl)-N-ethylisoluMinol [CheMiluMinescence Reagent], 97%
POLY(DIBUTYLTITANATE)
(+/-)-1-(4-BROMOPHENYL)ETHYL ISOCYANATE
3-(3,4-METHYLENEDIOXYPHENYL)PROPIONIC ACID
Bis(2,2,6,6-tetramethyl-3,5-heptanedionato)zinc, 99% [Zn(TMHD)2]
Isophthalic Acid
(2-methoxyethyl)(2-pyridinylmethyl)amine(SALTDATA: HCl)
1-methylcyclopentanecarboxylic acid(SALTDATA: FREE)
6-Bromoindole-3-carboxaldehyde
4-Methylcatechol
Propidium iodide
Methylcyclopentane
N1-(4-AMino-2-Methylphenyl)acetaMide
Propyl Benzoate
Methyl 2-chloro-5-hydroxybenzoate
4-Vinylaniline, 90%, tech.
Ursodeoxycholic acid, 99%
RESINIFERATOXIN
PentafluorophenylethoxydiMethylsilane
isoimperatorin
2-[(1S,2S)-1-Ethyl-2-bezyloxypropyl]-2,4-dihydro-4-[4-[4-(4-hydroxyphenyl)-1-piperazinyl]phenyl]-3H-1,2,4-triazol-3-one
6-D-Ala-10-D-gly-LHRH-ethylamide
Sodium triacetoxyborohydride
2-(DIFLUOROMETHYLTHIO)BENZOYL CHLORIDE
CINCHONINE MONOHYDROCHLORIDE HYDRATE 9&
3,5-DIHYDROXYBENZONITRILE
2-BroMo-6-(pyrrolidin-1-yl)pyridine
3,4-Benzopyrene
3-(4-Methoxyphenyl)propionic acid
2-Methyl-5-(piperidin-1-ylsulphonyl)-3-furoic acid 95%
2-Ethyl-2-methyl-1,3-propanediol
2,4,5-TRIMETHYLBENZALDEHYDE
Naphthionic acid
N-Cbz-L-glutamic acid
CaMphene;2,2-DiMethyl-3-Methylenenorbornane; 2,2-DiMethyl-3-Methylene bicyclo[2.2.1-heptane; 3,3-DiMethyl-2-MethylenenorcaMphane;
Tetracontane
11H-Benzo[a]fluoren-11-one
2-Fluoro-5-nitrophenylboronic acid
DiMethyl 1,4-cyclohexanedione-2,5-dicarboxylate, 99+%
3,4-PyridinedicarboxiMide, 97%
N-[(9H-Fluoren-9-ylMethoxy)carbonyl]-L-alanine Hydrate
Methoxyhydroquinone
1,15-Pentadecanedioic acid
2-(chloromethyl)-1,4-dioxaspiro[4.5]decane
Mozavaptan
(3S,4S)-(+)-1-Benzyl-3,4-pyrrolidindiol
GRP ELISA Kit
Mouse α-L-iduronidase,IDUA ELISA Kit
Human EGR3 (Early Growth Response Protein 3) ELISA Kit
Human DR-70TM ELISA Kit
Chicken prolactin,PRL ELISA Kit
Rat TAF1 (Tata Box Binding Protein Associated Factor 1) ELISA Kit
Chicken Laminin,LN ELISA Kit
Human matuRation promoting factor,MF/MPF ELISA Kit
Human Vascular Endothelial cell Growth Factor D,VEGF-D ELISA Kit
AGTR2 ELISA Kit
Medium 4(Yeast Beef Agar)
FM Agar, Modified
Diethyl isobutylMalonate, 98%
H-GLY-DL-ASP-OH H2O
5-Chloro-2-hydroxynicotinic acid, 97%
2-Fluoro-N-(3,5-difluorophenyl)benzaMide, 97%
5-Nitrouracil
C.I.Pigment Red 122
Potassium alginate
5-Fluoro-2-methoxybenzoic acid
3-Iodobenzyl bromide
N-CAPRYLIC ACID ISOBUTYL ESTER
1-(1,3-Thiazol-2-yl)Ethan-1-One
Methyl hydrogen isophthalate, 97%
(^+)-2-AMino-1-propanol, 98%
1-AMino-2-naphthol hydrochloride
SulfadiMethoxine
7-(TrifluoroMethyl)indoline-2,3-dione
PolyvinylforMal
CERIUM TUNGSTATE
SUBERIC ACID MONOMETHYL ESTER
4-Fluorobenzenesulfonyl chloride, 98%
4'-Chloropropiophenone
3-Amino-5-nitrobenzeneboronic acid, pinacol ester 97%
2-Fluoro-5-nitropyridine