MuM-2C细胞系—人侵袭性脉络膜黑色素瘤细胞系全国减价 货号:GD-C16819046
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
常见细胞培养中一些问题总结:1)培养基中血清的比例多少合适?血清是细胞培养上一个极为重要的营养来源,所以血清的含量多少会对细胞的生长会产生极大的影响。血清的含量一般为5%-15%,不要低于5%或高过20%,因为含量过低会导致细胞营养不足,过高则会破坏渗透压平衡。一般建议血清含量为10%,可以适量加减;2)何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上的更换时间,按时更换培养基即可。还可以参照指示剂的颜色变化进行更换;3)购买的复苏细胞系—为何会有脱落?因为运输的过程中不可避免的会有震荡,收到后可以离心收集;4)购买的细胞冷冻管经解冻后,为何会发生细胞数目太少之情形?研究人员在冷冻细胞之培养时出现细胞数目太少,大都是因为离心过程操作上的失误,造成细胞的物理性损伤,以及细胞流失。建议细胞解冻后不要立刻离心,应待细胞生长隔夜后再更换培养基即可;5)购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,常见原因可归纳为:培养基使用错误或培养基品质不佳。血清使用错误或血清的品质不佳。解冻过程错误。冷冻细胞解冻后,加以洗涤细胞和离心。悬浮细胞误认为死细胞。培养温度使用错误。细胞置于–80°C太久;6)收到的冷冻管瓶身破裂,瓶盖有裂纹,或瓶盖脱落之原因?冷冻管瓶盖裂纹,或瓶身破裂,可能是因为操作者夹取冷冻管时用力不当,造成冷冻管裂损,建议使用止血钳小心夹取。另冷冻管瓶盖松动或松脱,乃因热胀冷缩之物理现象,冷冻管有可能因此而造成细胞污染,故冷冻管于放入和取出液氮桶时,均应立刻将冷冻管再一次扭紧。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
3-(1H-Tetrazol-5-yl)phenol, 97%
1-Methyl-3-pyrrolidinol
BenzyltriMethylsilane, 98%
DiMethyl trisulfide
4-AMinophthaliMide
Methyl 3-Fluoro-2-thiophenecarboxylate
Phenyl isocyanatoforMate
Palladium(II) chloride, (trace metal basis), 99.999%
4-Isocyanato-3-methyl-5-phenylisoxazole , 90%
6-Chloro-3-formylchromone, 97%
1,5-Anhydro-D-glucitol
2-Chloro-4'-fluorobenzophenone
Z-ASP(OME)-OH
2-Ethoxycarbonylphenylboronic acid pinacol ester
2-Chloro-5-nitrobenzotrifluoride
Cyclohexyl Mercaptan
Decahydro-2-naphthyl ForMate (Mixture of isoMers)
Ethyl 4-broMo-3-nitrobenzoate
Trifluoroacetic acid 2,2,2-trifluoroethyl ester, 98%
PotassiuM Thiocyanate, Reagent, ACS
N-(Phenylthio)phthaliMide
3,5-dibromoanthranilic acid
3H-pyrrolo[3,2-d]pyrimidin-4(5H)-one
cis-4-tert-butylcyclohexanol
Thiocytosine
2-AMINOTEREPHTHALIC ACID
4,4'-Dihydroxytetraphenylmethane
ChloroforMic Acid AMyl Ester
(S)-1-(3-BroMophenyl)ethylaMine, ChiPros|r 99%, ee 98+%
Citraconic acid, 98+%
FMoc-Phe(4-Me)-OH
1,10-Phenanthroline hydrochloride Monohydrate
4-TERT-BUTYLCALIX[8]ARENE
POTASSIUM ALLYLTRIFLUOROBORATE
N-BenzylMaleiMide
3-Cyano-2-fluoropyridine
Hydroxypropyl Acrylate (mixture of 2-Hydroxypropyl and 2-Hydroxy-1-methylethyl Acrylate) (stabilized with MEHQ)
Cyclohexylmagnesium bromide [1M solution in THF]
Bis(pentamethylcyclopentadienyl)titanium dichloride, 99%
2-Methylpropylhydrazine hydrochloride
4,4'-DIAMINOSTILBENE DIHYDROCHLORIDE
HEXACHLOROBENZENE
1-Ethynyl-4-pentylbenzene
Toosendanin
4-AcetaMidothiophenol
p-Arsanilic acid
2-Isobutyl-3-Methylpyrazine
5-BroMo-2-ethylaMino-3-nitropyridine
N-BOC-AllylaMine, 98%
BOC-GLY-OSU
3-Chloro-2,4,5,6-tetrafluoropyridine
Bisphenol-A Bis(Diphenyl Phosphate)
6-Nitroindoline
1,3-dimethyl-1H-pyrazole-5-carboxylic acid
2,6-Dichloro-7-methylpurine
1-Methyl-4-piperidone
PENTAERYTHRITOL DIOLEATE
4-FLUORO-2-IODOTOLUENE
7-Methylindole
2-Fluoro-5-hydroxymethylpyridine
Dilauroyl peroxide, 99%
(pyrazolo[1,5-a]pyridin-3-ylmethyl)amine(SALTDATA: FREE)
(S)-3-Amino-1-carbobenzoxypyrrolidine Hydrochloride
4-Chlorobenzyl Mercaptan
N-FLUORO-4-METHYLPYRIDINIUM-2-SULFONATE
Aluminium glycinate
Ethylthiocyanate
Tritolyl phosphate
1,6-Diphenyl-1,3,5-hexatriene
(S)-(-)-6,7-DiMethoxy-1,2,3,4-tetrahydroisoquinoline-3-carboxylic Acid Hydrochloride
4-(DiMethoxyMethyl)pyriMidine
5-BroMo-1H-benziMidazole, 97%
1,4-DINITROBENZENE
1-Benzoyl-4-piperidone
N-Butaldimethylchlorosilane
Isoquinoline-4-carboxylic acid
Diethyldithiocarbamic acid diethylammonium salt
2-Methylacetophenone
CK 17 ELISA Kit
α-MSH ELISA Kit
Human MUC13 ELISA Kit
Human Lung resistance-related protein,LRP ELISA Kit
Chicken BMP-2 (Bone Morphogenetic Protein 2) ELISA Kit
S100A11 ELISA Kit
IL3 ELISA Kit
Human delta sleep-inducing peptide,DSIP ELISA Kit
Human VEGFC ELISA Kit
STAT6 ELISA Kit
Aerobe- Anaerobic Agar Medium
Yeast Synthetic Drop-out Agar Medium
Isobutyl isobutyrate, 98%
TRIHEPTADECANOIN
5-Chloro-8-hydroxyquinoline
2-Fluorobenzyl Cyanide
3,5-dibromoanthranilic acid
Acid Violet 48
zinc iodate
4-fluoroindole
1,4-Dibromopentane
PERFLUOROPENTADECANE
Tris-(4-Iodophenyl)AMine
N-Methyl-1-(2-thienyl)ethylaMine hydrochloride
DiMethyl selenide
Z-D-Met-OH
(TriMethylsilyl)diazoMethane
PHENOXYACETIC ANHYDRIDE
4'-N-HEXYLACETOPHENONE
SODIUM SULPHIDE HYDRATE
N-UNDECYLCYCLOHEXANE
6-Methylthionicotinamide
1-Benzhydryl-4-methyl
Didodecyl phthalate, 98%
β-D-Thiogalactose sodium salt ,98%