SUME-a细胞系—人鼻咽癌细胞系国外引进 货号:GD-C16719268
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞系—其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
3-BroMo-4-Methoxybenzoic acid, 98+%
4-BroMo-3,5-difluoroanisole
Propyl isocyanate
9,9-Bis(4-broMophenyl)fluorene
1,2-DibroMobenzene
2-(BENZYLOXY)-3-METHOXYBENZALDEHYDE
2-BroMotetradecane, 95%
5-amino-1-propyl-1H-imidazole-4-carboxamide(SALTDATA: FREE)
(S)-N-Boc-(2'-Chlorophenyl)glycine
1,2,4-Triaminobenzene Dihydrochloride
4-(Isobutyramido)benzeneboronic acid 98%
1,3-DIPHENOXYBENZENE
4-Bromo-1-fluoro-2-nitrobenzene
4-AMINO-TEMPO
1,2-CyclohexanedicarboxiMide
2-Acetoxyisobutyryl chloride
3,4'-Dihexyl-2,2'-bithiophene
3-BroMo-4-ethoxybenzaldehyde
Sudan Orange G, pure
SulfaMethizole
Isopropyl Acetoacetate
3-Nitrobenzamide
N-Benzylglycine ethyl ester hydrochloride
3-Acetyloxypregn-16-en-20-one
2,4-Pyridinedicarboxylic acid
9-HYDROXY-4-METHOXYACRIDINE
CARPROPAMID
Methyl Crotonate
SodiuM octanoate, 96%
2-Chloro-5-Methoxybenzoic acid, 98%
2-NP-AOZ
β-Gentiobiose
3-(Piperidine-1-carbonyl)phenylboronic acid, pinacol ester
O-Acetyl-N-carbobenzoxyhydroxylaMine
2-AMino-3-broMo-5-chloro-4-Methylpyridine
2-Acetoxyphenylboronic acid, pinacol ester
N-BOC-4-Chloroaniline, 98%
4-Benzyloxybromobenzene
1H,1H,2H,2H-Perfluorooctyl acrylate 95%
Disodium Citrate Sesquihydrate
Hexaethylcyclotrisiloxane
PHTHALIMIDOMALONIC ACID DIETHYL ESTER
3-Methylphthalic anhydride
1-PROPOXY-2-PROPANOL
Triphenylphosphine
L-Tyrosine ethyl ester hydrochloride
2,2,6,6-TetraMethyl-4-piperidyl Methacrylate
2-Furoic acid, 98%
AMMoniuM hexafluorosilicate, 99.999%, (trace Metal basis)
BOC-N-methyl-D-alanine
3,5-Dichlorobenzoic Acid
3-(4-BROMOBENZYL)-2-BUTYL-4-CHLORO-1H-IMIDAZOL-5-YLMETHANOL
2-chloroisonicotinamide
N-(3-Butenyl)phthalimide
3-chloro-6-methyl-dibenzo[c,f][1,2]thiazepin-11(6H)-one 5,5-dioxide
1,4-DICHLORO-5,6,7,8-TETRAHYDRO-5,8-ETHANOPHTHALAZINE
TRIETHYL 2-PHOSPHONOBUTYRATE
TRANS-2-OCTENOIC ACID
γ-(2-Naphthyl)-γ-butyrolactone
3-[(4-Methylpiperazin-1-yl)methyl]benzaldehyde, 95%
2-Butyl-4,4,5,5-tetramethyl-[1,3,2]dioxaborolane
1,5-Diiodopentane (stabilized with Copper chip)
2-Thiopheneglyoxal hydrate 95%
Acetonitrile
Flunixin meglumin
Dichloromonofluoromethane
TopiraMate
2-BroMoethyl ether
1-BroMotridecane
2-Methoxy-5-nitrobenzonitrile
6-Methoxyquinoline, 98%
4-Phenyltetrahydropyran-4-carboxylic acid, 97%
4-NitrodiazoaMinobenzene
p-Naphtholbenzein
phytosphingosine hydrochloride
2-(Boc-amino)-4-bromopyridine
2,4-Dichloro-5-fluorobenzaldehyde
Methyl-13C-amine hydrochloride
AQP1 ELISA Kit
RORC ELISA Kit
Human PCDHα1 ELISA Kit
Human DPP10 (Dipeptidyl Peptidase X) ELISA Kit
CD4 ELISA Kit
Rat amyloid beta peptide 1-42,Aβ1-42 ELISA Kit
GS-ANA ELISA Kit
Human ELA3A ELISA Kit
Human Serum amyloid A,SAA ELISA Kit
INHβA ELISA Kit
Urease Agar Base
Modified Phosphate Saline Buffer
Zinc(II) Acetylacetonate
LutetiuM(III) fluoride, 99.9% (Metals basis)
5,5',6,6'-Tetrahydroxy-3,3,3',3'-tetraMethyl-1,1'-spirobisindane, 97%
2-Fluoro-3-Methylpyridine, 97%
3-Nitrobenzamide
2',3',4'-Trichloroacetophenone
Aluminum oxide
(E)-2-Buten-1-ol
1,2-O-Isopropylidene-alpha-D-xylofuranose
PHENETHYLZINC BROMIDE
5-Fluoro-2-nitroanisole
3-BroMopropylboronic acid pinacol ester, 98%
3-Fluoro-4-Methoxyphenylacetonitrile, 99%
Ioxynil-octanoate
2′,3′-Dideoxyuridine
3,4-Dihydro-7-hydroxy-2(1H)-quinolinone
4-Propoxy-3-Methylphenylboronic acid
BISPHENOL A DIMETHACRYLATE
3-(DipropylcarbaMoyl)phenylboronic acid
tert-Butyl 4-(3-aminobenzyl)-1-carboxylate, 97%
2,6-Dimethyl-4-methoxybenzeneboronic acid
2,5-Difluoro-4-methoxybenzonitrile ,99%
Ethyl 3-(trifluoromethyl)benzoate