Raw246.7细胞系—小鼠单核巨噬细胞系国外引进 货号:GD-C16720555
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤:【瘤细胞悬液接种】1)无菌选取生长良好(有光泽,淡红色)瘤组织或对数生长期培养瘤细胞;2)在PBS中将瘤组织剪碎后用匀浆器研磨,经80~100目筛网过滤成细胞悬液;3)培养细胞应用PBS洗两遍;4)计数并调整细胞浓度至107~108/ml;5)常规消毒后,于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位,>106细胞)。初次接种成功率低,细胞数尽可能多一些;6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期,以后随着传代潜伏期逐渐缩短,固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位,引起腹水,腹水中含有瘤细胞系—将这种腹水反复传代,即可成为腹水瘤。初次传代时,腹水常呈血性(含大量红细胞),反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤,进行细胞计数;2)消毒动物,左下腹穿刺接种106腹水瘤细胞;3)接种腹水瘤细胞后约7~12d,待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部,用9号针头抽取腹水,也可行腹部解剖后,用滴管吸取。每只小鼠可抽3~5ml;4)抽取的腹水经3000rpm离心15min,收集上清,分装冻存备用。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
3-Methoxy-5-(trifluoroMethyl)benzoic acid, 97%
(S)-3-(Boc-aMino)piperidine
2-Piperidinoethanol
4-BUTYLBENZONITRILE
4-BroMo-3-fluorotoluene
TopiraMate , 98%
4-Methyldiphenylmethane
1-(2-Ethoxyphenyl) Hydrochloride
2-Bromo-4-nitroanisole
Octaethylene Glycol Monododecyl Ether
1-Bromo-3-(1,1,2,2-tetrafluoroethoxy)-benzene
N-Boc-N-methylethylenediamine
CYCLOPROPYL DIPHENYL CARBINOL
2-Bromobenzyl cyanide
2-(4-Benzylpiperidino)-1-ethanaMine
1-(BroMoMethyl)-3-(trifluoroMethoxy)benzene
1-(2-Deoxy-2-fluoro-beta-D-arabinofuranosyl)-5-iodouracil
Tert-butyl 4-broMo-2-fluorobenzoate
(?)-2-Ethoxycyclohexanone, 98%
Tyrosinase, Lyophilized powder
D-(+)-Galactose Anhydrous
Tributylphosphine oxide
Cyanidin 3-O-glucoside
Polyaluminium Chloride
alpha,alpha'-diamino-P-xylene
N-BENZYLMALEAMIC ACID
CI 42755
SodiuM Tripolyphosphate
4-tert-Butylbenzyl broMide, 97%
3,4,5-TrihydroxybenzaMide, 98%
9,9-Di-(2′-ethylhexyl)-2,7-dibroMofluorene
1-Butanethiol
D-2,4-DICHLOROPHENYLALANINE
N-LAUROYLSARCOSINE
(2,2-DIMETHYL-[1,3]-DIOXOLAN-4-YL)-METHYLAMINE
Lauryl acrylate
2,3,5,6-Tetrafluoro-4-mercaptobenzoic Acid
N-(4-Aminophenyl)nicotinamide
1-Boc-Piperidin-4-ylpropionic acid
4,5-Bis(hydroxymethyl)imidazole
Adenine hydrochloride
1-(2,4-DIMETHOXYPHENYL)
4-Methylpyrazole
2-NAPHTHYL ISOCYANIDE 97
DiiodoMethane (stabilized with Copper chip)
3-(2-Pyridyl)-alanine
Trihexylsilane
2,5-Dichloronitrobenzene, 99%
4,4,6-TriMethyl-2-vinyl-1,3,2-dioxaborinane, 98%, stabilized
Cinchonidine
Phenylsulfonylacetonitrile
tert-butyl (R)-(+)-4-formyl-2,2-dimethyl-3-oxazol
Acetamide, N-[(2R)-2-acetyl-1,2,3,4-tetrahydro-5,8-dimethoxy-2-naphthalenyl]-
4-methyl-3-nitro-1H-pyrazole
1,3,5-tribromo-2-iodobenzene
METHYL BETA-(DIMETHYLAMINO)ISOBUTYRATE
2-BroMo-1-Methyl-4-(trifluoroMethylsulfonyl)benzene
CALCIUM PHOSPHATE TRIBASIC
(RP,R′P)-1,1′-Bis[bis(2-Methylphenyl)phosphino]-2,2′-bis[(S)-α-(diMethylaMino)benzyl]ferrocene
3,5-dichloro-4-(prop-2-yn-1-yloxy)benzaldehyde(SALTDATA: FREE)
Resiniferatoxin, high purity, 99%
2,5-Dimethyl-2,5-di(tert-butylperoxy)hexane, 92%
5-Nitro-2-furancarboxylic Acid
N-ETHYL-4-CHLOROANILINE
2-Allylphenol
5-AMINO-2-(4-TERT-BUTYLPHENOXY)PYRIDINE
Triazol-3-amine
1,2,4-Benzenetricarboxylic anhydride
1H-Pyrrolo[3,2-c]pyridine
Tetrafluorophthalonitrile
2,5-DiMethylpyrrolidine, 97%
IMidazo[1,2-a]pyridine-6-carbonyl chloride hydrochloride, Tech.
Methyl 2-methylbutyrate
2,3,4,5,6-PentabroMotoluene
Kathon 886
Ciprofloxacin Monohydrochloride
3-(4-Pyridyl)aniline
2-(2'-Hydroxy-3'-5'-di-tert-amylphenyl)benzotriazole
NRG-1 ELISA Kit
Mouse NTXI (Cross Linked N-Telopeptide of Type I Collagen) ELISA Kit
Human INMT ELISA Kit
Human GALNT14 ELISA Kit
Monkey cTn-I/TNNI3 (cardiac Troponin I) ELISA Kit
Orexin A ELISA Kit
VCAM-1/CD106 ELISA Kit
Human PKD2 ELISA Kit
GP-4 ELISA Kit
Strail ELISA Kit
LB Agar,Lennox
Levine’s Eosin-Methylene Blue Agar without Lactose
EuropiuM(III) acetate hydrate, 99.99%
Molecular sieves, , 3-5MM (0.12-0.20in) beads
4-n-Pentylbenzaldehyde, 95%
2-Iodo-1-trityliMidazole, 97%
Tributylphosphine oxide
tetratriacontane
Pigment Yellow 62
1-Bromo-2-naphthol
2,6-Dimethylbenzoic acid
4-METHOXY-2(5H)-FURANONE
(+/-)-2-AMinobutane
1-BroMo-4-phenylbutane, 98%
4-Ethylbenzaldehyde, 97%
4-Chloro-7-nitro-1,2,3-benzoxadiazole
MBTH hydrochloride hydrate
1-BroMo-4,4-diMethylcyclohex-1-ene
4-BroMo-N-ethyl-2-fluoroaniline HCl
4-Carboxynaphthalene-1-boronic acid
2,2,6-TRIMETHYLCYCLOHEXANONE
Esculetin
Diphenylborinic acid 2-aminoethyl ester, 98%
2-Methoxy-5-trifluoromethoxy-benzonitrile
Methoxybenzoquinone