MA891细胞系—小鼠乳腺癌高转移细胞系国外引进 货号:GD-C16720541
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤:【瘤细胞悬液接种】1)无菌选取生长良好(有光泽,淡红色)瘤组织或对数生长期培养瘤细胞;2)在PBS中将瘤组织剪碎后用匀浆器研磨,经80~100目筛网过滤成细胞悬液;3)培养细胞应用PBS洗两遍;4)计数并调整细胞浓度至107~108/ml;5)常规消毒后,于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位,>106细胞)。初次接种成功率低,细胞数尽可能多一些;6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期,以后随着传代潜伏期逐渐缩短,固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位,引起腹水,腹水中含有瘤细胞系—将这种腹水反复传代,即可成为腹水瘤。初次传代时,腹水常呈血性(含大量红细胞),反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤,进行细胞计数;2)消毒动物,左下腹穿刺接种106腹水瘤细胞;3)接种腹水瘤细胞后约7~12d,待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部,用9号针头抽取腹水,也可行腹部解剖后,用滴管吸取。每只小鼠可抽3~5ml;4)抽取的腹水经3000rpm离心15min,收集上清,分装冻存备用。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
PotassiuM pyriMidine-5-trifluoroborate, 95%
1,2,3,5-Tetrachlorobenzene
2',4',6'-TRIFLUOROACETOPHENONE
1,2-BIS(DIMETHYLPHOSPHINO)ETHANE
5-(2-NITROPHENYL)FURAN-2-CARBONYL CHLORIDE
1-AMino-7-naphthol
3-Bromo-1-(triisopropylsilyl)indole
1H-Benzimidazole-2-ethanol
2-chloro-3-(1-pyrrolidinyl)pyrazine(SALTDATA: FREE)
Boc-S-methyl-L-cysteine
2-Bromoethyl Benzoate
Sodium cromoglycate
L-Histidine hydrochloride
2,3,3',4'-BIPHENYL TETRACARBOXYLIC DIANHYDRIDE
2-MethylcinnaMic acid
Dipropyl disulfide
4-(4,5-Diphenyl-1H-iMidazol-2-yl)phenol [for BiocheMical Research]
N-Propyl 4-(4-broMopyrazol-1-yl)benzenesulfonaMide
4-BroMo-2-(trifluoroMethoxy)benzene-1-thiol, 95%
TriMethopriM, Powder, USP
5-Methyluridine
1,1,3,3-Tetraphenyldimethyldisiloxane
(2S,4S)-4-Fluoropyrrolidine-2-carboxylic acid
Pigment Blue 10
Dibenzylideneacetone
Sebacic acid
TRIS(DIBENZYLIDENEACETONE)DIPALLADIUM(0)
3-ChlorobenzylaMine
tert-Butyl pivalate, 98%
2,4-Dichloro-5-Methylpyridine, 96%
Cortisone
N,N-DiisopropylethylaMine
4-Chloro-2'-broMoacetophenone
N-Methyl 4-fluoro-2-(trifluoroMethyl)aniline, HCl
4-BROMO-2-CHLOROBENZENESULFONYL CHLORIDE
9,9-Dihexyl-2,7-dibroMofluorene
4-Methyl-3-nitrobenzonitrile, 97%
Pamoic acid, 98+%
Ethyl (S)-(+)-2-pyrrolidone-5-carboxylate, 99%
5-isopropyl-1H-pyrazole(SALTDATA: FREE)
Amstat
2-Cyano-3,5-difluoropyridine
Actarit
AluMinuM trichloride anhydrous;AluMiniuM chloride;AluMinuM trichloride
(2-Methylphenyl)Methanesulfonyl chloride
FMoc-Cys(pMeOBzl)-OH
N-Phenylglycine PotassiuM Salt
Pyrido[2,3-d]pyriMidine-2,4(1h,3h)-dione
5-AMinoindan-1-one, 97%
ERYTHROSIN B
4-(N-Butyl-N-nitrosaMino)butyric Acid
Iminostilbene carbonyl chloride
2,3-dihydrobenzofuran-5-carboxaldehyde
2-(Bicycloheptyl)trichlorosilane
Microcrystalline Cellulose
2-CHLORO-6-FLUORO-3-METHYLBENZOIC ACID
(Z)-2-BroMo-2-butene
N-(3,5-DibroMophenyl)pivalaMide
3-(4-BroMophenyl)-5-Methyl-1,2,4-oxadiazole
3-(Pyridine-4-carbonyl)benzenesulfonyl chloride
3-Fluoro-4-(trifluoromethyl)benzyl bromide 97%
Methyl 3-O-(2-acetamido-2-deoxy-β-glucopyranosyl)-β-D-galactopyranoside ,98%
Potassium hexafluorogermanate
Flufenoxuron
4,4'-Isopropylidenedicyclohexanol
DL-BENZYLSUCCINIC ACID
Disodium edetate dihydrate
4-(Methylthio)phenol
1-Heptyn-3-ol
O-(2,3,4,5,6-Pentafluorobenzyl)forMaldoxiMe
(IodoMethyl)triMethylsilane, 99%
Tetrahydro-2H-pyran-4-carboxylic acid, 97%
LITMUS
N,N-Diethyl-p-toluidine
5-bromo-2-chloro-4,6-dimethylpyridine-3-carbonitrile
Direct Yellow 96
3-Chloro-2-hydroxy-5-nitropyridine
Isosorbide-2-acetate
NAIP ELISA Kit
MKI67 ELISA Kit
Human STRC ELISA Kit
Human PYY (Peptide YY) ELISA Kit
Monkey PECAM1/CD31 (Platelet/Endothelial Cell Adhesion Molecule 1) ELISA Kit
γ-GCS ELISA Kit
NEOP ELISA Kit
Human PKCζ (Protein Kinase C Zeta) ELISA Kit
Human PA-IgA ELISA Kit
NAP-2 ELISA Kit
Lactose Monohydrate Broth
Phosphate solution(1M,pH6.0)
Ethanesulfonyl Chloride
Phenol Red sodium salt
4-n-Octylphenol, 99%
5-Chloro-2-Iodopyridine
1,1,3,3-Tetraphenyldimethyldisiloxane
acetonitrile-13C2-15N
Acid brown 58
Dithiobiuret
CARMINE
1-ADAMANTANECARBOXAMIDE
2,3-Lutidine
Bis(diphenylphosphino)Methane, 97%
1-Propynyltri-n-butyltin, 96%
(S)-(+)-2-(AnilinoMethyl)pyrrolidine
Pituitary adenylate cyclase activating polypeptide-38
Thio-L-citrulline
3-CYANOCHROMONE
Chloro[[1,3-bis(2,6-diisopropylphenyl)iMidazol-2-ylidene](N,N-diMethyl-3,5-diMethoxybenzylaMine)palladiuM(II)]
2,4-Dinitrotoluene
2-Bromo-4-fluorobenzyl alcohol
2,6-Difluorotoluene 99%
Ethyl 2-Oxo-4-phenylbutyrate
[N-[1-[2-(2-Pyridylcarboxamido)phenyl]ethylidene]glycinato]nickel