VERO C1008[Vero 76,clone E6,Vero E6]细胞系—非洲绿猴肾细胞系国外引进 货号:GD-C16720468
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤:【瘤细胞悬液接种】1)无菌选取生长良好(有光泽,淡红色)瘤组织或对数生长期培养瘤细胞;2)在PBS中将瘤组织剪碎后用匀浆器研磨,经80~100目筛网过滤成细胞悬液;3)培养细胞应用PBS洗两遍;4)计数并调整细胞浓度至107~108/ml;5)常规消毒后,于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位,>106细胞)。初次接种成功率低,细胞数尽可能多一些;6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期,以后随着传代潜伏期逐渐缩短,固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位,引起腹水,腹水中含有瘤细胞系—将这种腹水反复传代,即可成为腹水瘤。初次传代时,腹水常呈血性(含大量红细胞),反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤,进行细胞计数;2)消毒动物,左下腹穿刺接种106腹水瘤细胞;3)接种腹水瘤细胞后约7~12d,待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部,用9号针头抽取腹水,也可行腹部解剖后,用滴管吸取。每只小鼠可抽3~5ml;4)抽取的腹水经3000rpm离心15min,收集上清,分装冻存备用。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
3,5-Dinitrobenzoic acid, 98+%
Boc-D-lle-OH
ACETIC ACID (2R,3R,4R,5R)-3,4-DIACETOXY-5-(2-AMINO-6-CHLORO-PURIN-9-YL)-TETRAHYDRO-FURAN-2-YLMETHYL ESTER
3-Chloro-4-(2-fluorobenzyloxy)phenylboronic acid
6-BroMo-1,3-dihydro-2H-indol-2-one
16,17-Epoxypregnenolone acetate
2,4-DIMETHYL-1,3-PENTADIENE
4-(2-Hydroxy-ethoxy)-benzaldehyde
4-(Trifluoromethoxy)iodobenzene 97%
N-(1-Methyl-3-phenylpropyl)valeramide
2-chloro-6-fluorobenzothiazole
5-ACETYLURACIL
Lithium chromate
PHENYL CARBAMATE
3,4-DiaMinothiophene dihydrobroMide
2,4,5,6-TetraaMinopyriMidine sulfate hydrate
3-(DiMethylaMino)-1-(2-thienyl)-1-propanol
Methyl 6-broMo-4-oxo-1,4-dihydroquinoline-2-carboxylate
4-Methylpyrazole, 97%
Testosterone Enanthate (CIII), BP, EP
cis-Hydrindane
3-Cyclohexylpropionic acid
5-Benzyloxyindole-2-carboxylic acid
dl-aspartic acid hemimagnesium
Azetidine
3,5-DIFLUOROPHENYLACETONITRILE
4-CYANOPHENYL 4-PENTYLBENZOATE
2,4-Thiazolidinedione
5-Hydroxyindole-2-carboxylic acid, 97%
4-Allyl-2,6-diMethoxyphenol, 98%
6-BroMo-2-pyridinecarboxaldehyde
PotassiuM p-tolyltrifluoroborate
N-4-BOC-N-1-CBZ-2- CARBOXYLIC ACID
N-PROPYL-2-METHYL BUTYRATE
(R)-4-AMino-2-Methyl-1-butanol , 98%
4-HYDROXY-3-(TRIFLUOROMETHYL)BENZALDEHYDE
3-Bromo-2-methylbenzoic acid
Ethyl isobutyrylacetate, 95%
tert-Butylmethoxyphenylsilyl Bromide
5-Chloro-2-mercaptobenzimidazole
2,6-DIISOPROPYLNAPHTHALENE
2',4',6'-TRIHYDROXYACETOPHENONE
(-)-2,3-O-Isopropylidene-D-threitol
EC 1.1.1.1
tert-Butyl N-(3-aMino-3-thioxopropyl)carbaMate
4-Hydroxybutyl Acrylate (stabilized with MEHQ)
tert-Butyl N-(5-broMopyridin-3-yl)carbaMate
4-(MethylaMino)butyric acid hydrochloride, 99%
3,3,3-Trifluoro-(2-tetrahydrofuranylMethyl)propionic acid, 95%, Mixture of diastereoMers
S-Adenosyl-L-methionine tosylate
TrifluoroMethanesulfonyl Chloride
dioctadecylamine
D(+)-Xylose
3,5-Dichlorophenyl Boronic Acid
4-hydroxy-1-indanone
Salicylaldoxime
ACETYLTHIOCHOLINE IODIDE
1-Vinylnaphthalene (stabilized with TBC), 95%
NiobiuM potassiuM isopropoxide, 2.5% w/v in isopropanol, 99% (Metals basis)
L(+)-allo-Threonine
3'-Chloroindophenol Sodium Salt [Redox Indicator]
1-(Chloromethyl)-3,5-bis(methylsulfonyl)benzene
Hafnium(IV) dichloride oxide octahydrate (98+%-Hf, 1.5% Zr)
Entecavir
Methyl 2-methyl-3-furyl disulfide
5-FLUORO-2-METHYLBENZYL BROMIDE
Sodium antimonate
Pyrido[3,2-d][1,3]thiazole-2-thiol
1-Cbz-4-piperidinecarboxylic Acid
Methyl 2,3,6-Tri-O-benzoyl-alpha-D-galactopyranoside
2-Fluoro-3-Methoxyaniline
2-(BroMoMethyl)-6-pyrrolidin-1-ylpyridine, 97%
Bromoacetyl chloride
4-HydroxydiphenylaMine
(4-Carboxybutyl)triphenylphosphonium bromide
Popcorn aroma
5-Hydroxy-2-methylindole
D-mannosamine hydrochloride
Porcine Orexin receptor,OXR ELISA Kit
PDAP1 ELISA Kit
iNOS ELISA Kit
Human DPYS ELISA Kit
CIC ELISA Kit
β-EP ELISA Kit
CORT ELISA Kit
Protein S ELISA Kit
Human TX-A2 ELISA Kit
PGR ELISA Kit
Starch Hydrolysis Medium
Purple Broth Base
Acetylacetone Cobalt(II) Salt
Fluorobenzene
5-(4-tert-Butylphenyl)-1,3,4-oxadiazole-2-thiol, 96%
2-Buten-1-ol, cis + trans (ca 1:19), 96%
3-Cyclohexylpropionic acid
quinoline-3-carboxaldehyde
Ammonium paratungstate
Rosmarinic acid
ALPHA-METHYLSTILBENE
1,2-Dimethyl-5-nitroimidazole
Octyl Butyrate
2-BroMoisobutyryl broMide, 97%
5-Nitro-2-furoic acid, 98+%
2-Benzoxazolinone
Fenbutatin oxide
N-(4-Pyridyl)pyridiniuM chloride hydrochloride
3-PHENYLSALICYLIC ACID
3-ETHYL-5-METHYLISOXAZOLE-4-CARBOXYLIC ACID
5-Methyl-3-phenylisoxazole-4-carboxaldehyde
2-Chloro-4-nitrobenzenecarbonitrile
1-Chloro-2-iodoethane (stabilized with Copper chip)
N-(3-methylbenzyl)propan-2-amine(SALTDATA: HCl)
trans-Perfluorodecalin