C3H/10T1/2细胞系—小鼠纤维原细胞系来电咨询更多惊喜

C3H/10T1/2细胞系—小鼠纤维原细胞系来电咨询更多惊喜 货号：GD-C16619212

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息，我们拥有丰富的细胞系培养经验，能提供细胞培养全方位的技术支持，让您实验再无烦扰！公司由在国外工作和学习多年的留学人员创办，由国内ZG科学院上海生物化学与细胞生物学研究所，复旦大学，同济大学等多名工作在线的细胞培养专家，提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念，以世界的技术支撑阵容，提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美，提供了重要的基石；公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品，立足国内，面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位，为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内，在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室，以便更好的服务国内广大医院、大学、制药企业等，你们的每一份关心和支持，都是我们前进的动力！
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，原因比较复杂，常见原因可归纳为：培养基使用错误或培养基品质不佳；血清使用错误或血清的品质不佳；解冻过程错误；冷冻细胞解冻后，加以洗涤细胞和离心；悬浑细胞误认为死细胞；培养温度使用错误；细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来，其离心速率应为多少转速?欲回收动物细胞系—其离心速率一般为300×g(约1OOOrpm),5-10分钟，转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2，其中r为离心机转轴ZX与离心套管底部内壁的距离；rpm为离心机每分钟的转数；RCF(relative centrifugal force)为相对离心力，以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
3-Nitroaniline, 98%
N-MethyldiethanolaMine
2,2,5,5-TETRAMETHYL-4-PHENACETYLIDEN IMIDAZOLIDINE-1-OXYL FREE RADICAL
5-BroMobenzooxazole
Isobutyl chloride
(R)-N,N-DiMethyl-1-[(S)-1',2-bis(diphenylphosphino)ferrocenyl]ethylaMine , 98%
5-BroMo-2-chloropyriMidine
4-tert-Butylaniline
Phenanthridine
(4-Bromophenyl)succinic acid
3-Octadecylthiophene
2-(BUTYLAMINO)ETHANOL
1-METHYLPIPERIDINE-2-CARBOXYLIC ACID HYDROCHLORIDE
TRANS-3-ETHOXYCINNAMIC ACID
Phenolphthalein disulfate
IMidazo[1,2-a]pyridine-6-carbaldehyde
2,6-DibroModithieno[3,2-b:2',3'-d]thiophene
Benzyl cis-4-forMylcyclohexylcarbaMate
Vinylene carbonate, 98%, stabilized
SodiuM Sulfite, Reagent, ACS
Bis(trifluoro-2,4-pentanedionato)cobalt(II) Hydrate
cis-1,3-Dimethylcyclohexane
diethyl 4-oxopimelate
3,3-Tetramethyleneglutarimide
3-fluoropropiophenone
3-BROMO-3-METHYLPENTANE
1-Benzyl-3-piperidone
(R)-N-Boc-3-AMino-3-Phenyl-Propan-1-OL
2-(DiethylcarbaMoyl)benzeneboronic acid, 95%
4-(9-Carbazolyl)benzeneboronic acid, 98%
(R)-(+)-BINAP
1H,1H,2H,2H-Perfluorodecanethiol
Thiophene-2-ethylaMine
p-Arsanilic acid, 98%
2-ACETYL-5-NITROBENZO[B]FURAN
5-(t-ButyldiMethylsilyloxy)-2,3-difluorophenylboronic acid
Methyl 5-fluoro-2-nitrobenzoate
3-(2-Methoxyphenyl)-3-oxo-propionic acid ethylester
Z-Glu-OBzl
3-Chloro-4-iodotoluene
4-METHYL-1,3-DIOXANE
6-Chloropyridn-2-ol
2-Bromopentane
Diacetyl MonoxiMe;IsonitroMethyl ethyl ketone;Isonitroethyl Methyl ketone;Methyl isonitroethyl ketone
o-Toluidine
Oxalacetic acid
Ethyl 3-Hydroxyhexanoate
3-(4-Chloro-2-Methylphenyl)benzoic acid
4-Iodo-o-xylene, 97%
INDENO(1,2,3-C,D)PYRENE
L-(+)-2-(2-Chlorophenyl)glycine
4,6-Dichlorocresol
alpha-chymotrypsin from bovine pancreas
oxonic acid potassium salt
Androsta-1,4,6-triene-3,17-dione
4-BROMO-DL-PHENYLALANINE
2,7-DIBROMO-9H-CARBAZOLE
Boc-(S)-3-aMino-4-(4-trifluoroMethylphenyl)-butyric acid
2-PicolylaMine
2,4-Dimethyl-3-ethylpyrrole ,98%
4-Bromo-5-chlorothiophene-2-sulfonyl chloride , 95%
Copper(II) Bis(2-hydroxyethyl)dithiocarbamate
2-Fluoro-6-methylbenzaldehyde
1-beta-D-Arabinofuranosylcytosine hydrochloride
HEXABROMOBENZENE
MALEIC ANHYDRIDE
BISMUTH MOLYBDATE
2-BroMoaniline
1-BroMo-2-chloro-3-fluorobenzene
2,5-Dichloropyridine-4-boronic acid, pinacol ester
Copper(II) oxide, ACS reagent, powder
trans-1-CinnaMyl, 97%
6-Hydroxy-1-tetralone
1,2-Dihydroxynaphthalene
Dibutyl tin diacetate
3-(1H-PYRAZOL-3-YL)BENZOIC ACID
Quizalofop-p-tefuryl
1-n-Butyl-3-methylimidazolium chloride
PGF ELISA Kit
Slit3 ELISA Kit
Human Progesterone,PROG ELISA Kit
Human UBAP2 ELISA Kit
IL10 ELISA Kit
α Actin ELISA Kit
Chicken TIMP-2 (Tissue Inhibitors of Metalloproteinase 2) ELISA Kit
CETP/LTP ELISA Kit
Human HO1 ELISA Kit
IGF1 ELISA Kit
Saline Lactose Broth Medium
Bacteria Save Medium
Isopropyl acetoacetate, 98%
YttriuM Fluoride
5,6-Dihydroxyflavone, 97%
2-Fluoro-4-Nitroaniline
cis-1,3-Dimethylcyclohexane
Hydrazine acetate
hydroxyzine pamoate
5-hydroxy-4-oxo-4H-pyran-2-carboxylic acid

1-(2-BROMOETHOXY)-4-METHYLBENZENE
TRITON(R) X-100, HYDROGENATED
Di-n-Octyltin Oxide
2-AMinopyridine-5-Methanol, 97%
EthyltriphenylphosphoniuM acetate, 70% in Methanol
N,N-Dibenzylaniline, 99%
(S,S)-(+)-N,N′-Bis(3,5-di-tert-butylsalicylidene)-1,2-cyclohexanediaMine
DL-ALANYL-DL-ALANINE
5-AMINOTETRAZOLE MONOHYDRATE
p-Tolualdehyde
1-Chloroheptane
3-CHLORO-L-ALANINE HYDROCHLORIDE
Isobutyl isothiocyanate
Ethyl 1-Benzylpyrrole-3-carboxylate
6-Chloro-1H-pyrrolo[3,2-c]pyridine