VERO-76细胞系—非洲绿猴肾细胞系来电咨询更多惊喜 货号:GD-C16619200
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞系—其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
Pyrazine-2-carboxylic acid, 99%
SodiuM iodate
2-(CarbobenzoxyaMino)-1-ethanol , 97%
4-UNDECANONE
SodiuM pyruvate-2-13C
Direct yellow 50
SucciniMidyl (2R)-6-(Tetrahydro-2H-pyran-2-yloxy)-2,5,7,8-tetraMethylchroMan-2-carboxylate
Tris(trimethylsilyloxy)silane, 97%
h-d-glu-otbu
Tantalum(V) ethoxide (99.99+%-Ta) PURATREM
2-Heptene (cis- and trans- mixture)
VINYL IODIDE
1-Hydroxy anthraquinone
N,N'-(1,4-Phenylene)bis(acetoacetamide)
2-BroMoisobutyric acid
4-(4,6-DiMethoxy-1,3,5-triazin-2-yl)-4-MethylMorpholiniuM chloride
2,5-Dihydroxyphenyl(diphenyl)phosphine Oxide
4-Acetoxy-2-broMo-5-Methoxybenzaldehyde
VanadiuM(V) oxytriethoxide, 95%
SodiuM Polystyrene Sulfonate, USP
2-AcetaMidoacrylic Acid
tetrahydrofurfuryl propionate
4-Methoxyresorcinol
1-bromo-2,3,4,5-tetrafluorobenzene
4-Aminotrifluorotoluene
3-Nitrobenzonitrile
2,3-Dihydroxypyridine
Di-n-butyltin diacetate, 95%
1-Methylpyrrole-2-carbonyl chloride, 95%
3-AMinopyridine-4-carboxylic acid, 95%
Benzene sulfonyl chloride
TERBIUM SULFATE
(1R,2S,5R)-(-)-MENTHYL (S)-P-TOLUENESULFINATE
Pentadecanophenone
3,5-DifluorobenzylaMine
2,4-Diphenyl-4-Methyl-1-pentene
Sodium Deoxycholate [for Electrophoresis]
9,10-Bis(chloromethyl)anthracene
N,N-Dimethylnicotinamide-2-sulfonamide
2-Hydroxymethyloxetane
ERBIUM CHLORIDE
Primisulfuron
1,4-Benzodioxan
TERT-BUTYL ETHYL ETHER
Azelaic acid
2-Butyl-1,3-diazaspiro[4.4]non-1-en-4-one Hydrochloride
2,5-Difluoropyridinr-3-boronic acid
Cyclohexylbenzene, 98%
DiMethyl pyridine-2,5-dicarboxylate, 97%
DibroMofluorescein (contains Mono-, Tri- and Tetra-)
3-Pyridinecarboxaldehyde
Dibromotriphenylphosphorane
Ethyl 2-bromo-3-nitrobenzoate
3-(2-Quinolyl)-DL-alanine
6-bromo-1,3-benzodioxole-5-carboxamide
1-CHLORO-3,3-DIMETHYLBUTANE
3-Nitrobenzyl chloride
(S)-(+)-Glycidyl nosylate
5-AMino-4-chloro-2-fluorobenzoic acid, HCl
1-Chloro-2-methylpropyl chloroformate, 98%
2-Phenoxybenzoyl chloride , 90%
Octafluorocyclobutane (PFC C-318) 99%
Hexyl Ether
2,3-Dihydro-2,2-dimethyl-7-benzofuranol
(5R)-3-(3-Fluoro-4-(4-morpholinyl)phenyl)-5-hydroxymethyl-2-oxazolidione
trans-2,3-Dibromo-2-butene-1,4-diol
2-Methylindene
3-ThienylMethylaMine
1-BroMo-2-(broMoMethyl)naphthalene
DiMethyl 2-(5-Methyl-2,4-dinitrophenyl)Malonate
AMMoniuM iron(III) citrate, 16.5 to 18.5% Fe
5,6-Dichloronicotinic acid, 98%
4-Heptylbenzoic Acid
alpha-Methyl-DL-tyrosine Methyl Ester Hydrochloride
3,3-dimethyl-1-butanol
ethyl 2-(trifluoromethyl)pyrimidine-5-carboxylate
4-Methylnicotinic acid hydrochloride
4-(5,5,6-trimethylbicyclo[2.2.1]hept-2-yl)cyclohexan-1-ol
CA1 ELISA Kit
FYN ELISA Kit
Human progestin and adipoQ receptor family member Ⅶ,PAQR7 ELISA Kit
Human E2/ubiquitin-conjugating enzyme,E2/UBCE ELISA Kit
IL15 ELISA Kit
Rat Interferon α,IFN-α ELISA Kit
ROS ELISA Kit
Human cholesterol,CH ELISA Kit
Human HBθ1 ELISA Kit
NO ELISA Kit
Water Agar Medium
Soya Casein Digest Lecithin Polysorbate Broth Base
GalliuM(III) ethoxide
FLURBIPROFEN
5,7-Dichloro-8-hydroxyquinaldine
2-Fluoro-4-nitrobenzeneboronic acid pinacol ester, 96%
tetrahydrofurfuryl propionate
1,2,3-tris(2,3-epoxypropoxy)propane
2,2-Dibromo-3-Nitrilopropionamide
Isonipecotic acid
DIMETHYL PIMELATE
P-TOLYL ACETATE
Ethyl 3-Methyl-1H-Pyrazole-5-Carboxylate
2-sec-Butylthiazole, 98+%
4-AMino-3-nitrophenol, 98%
N-Phenyl-3-biphenylaMine
sec-Butylbenzene
4,4,4-TRICHLOROBUTYRIC ACID
2-HYDROXY-5-METHYLISOPHTHALALDEHYDE
N,N′-Diethyl-1,3-propanediaMine
4-BroMo-3-Methoxybenzoic acid
1-Bromo-2-methylhexadecane
3,4-Dichloroaniline
5-Ethyl-2-furaldehyde, 98%
trans-2-Undecen-1-ol