B16-F0细胞系—小鼠黑色素瘤细胞系来电咨询更多惊喜 货号:GD-C16619012
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
常见细胞培养中一些问题总结:1)培养基中血清的比例多少合适?血清是细胞培养上一个极为重要的营养来源,所以血清的含量多少会对细胞的生长会产生极大的影响。血清的含量一般为5%-15%,不要低于5%或高过20%,因为含量过低会导致细胞营养不足,过高则会破坏渗透压平衡。一般建议血清含量为10%,可以适量加减;2)何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上的更换时间,按时更换培养基即可。还可以参照指示剂的颜色变化进行更换;3)购买的复苏细胞系—为何会有脱落?因为运输的过程中不可避免的会有震荡,收到后可以离心收集;4)购买的细胞冷冻管经解冻后,为何会发生细胞数目太少之情形?研究人员在冷冻细胞之培养时出现细胞数目太少,大都是因为离心过程操作上的失误,造成细胞的物理性损伤,以及细胞流失。建议细胞解冻后不要立刻离心,应待细胞生长隔夜后再更换培养基即可;5)购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,常见原因可归纳为:培养基使用错误或培养基品质不佳。血清使用错误或血清的品质不佳。解冻过程错误。冷冻细胞解冻后,加以洗涤细胞和离心。悬浮细胞误认为死细胞。培养温度使用错误。细胞置于–80°C太久;6)收到的冷冻管瓶身破裂,瓶盖有裂纹,或瓶盖脱落之原因?冷冻管瓶盖裂纹,或瓶身破裂,可能是因为操作者夹取冷冻管时用力不当,造成冷冻管裂损,建议使用止血钳小心夹取。另冷冻管瓶盖松动或松脱,乃因热胀冷缩之物理现象,冷冻管有可能因此而造成细胞污染,故冷冻管于放入和取出液氮桶时,均应立刻将冷冻管再一次扭紧。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
Methyl 4,5-dibroMo-3-hydroxythiophene-2-carboxylate, 97%
2-Cyano-3,5-difluorpyridine
(R)-(+)-Propylene oxide
ADONITOL
Salicylaldehyde thioseMicarbazone
5-Chloro-2-(Methoxycarbonyl)phenylboronic acid
5-Chlorothiophene-2-boronic acid
L-Sorbitol ,98%
2-(ethylamino)pyrimidine-5-carbaldehyde(SALTDATA: FREE)
2-(Tetrazol-5-yl)phenylboronic acid
(4-methoxybenzyl)2-propyn-1-ylamine(SALTDATA: HCl)
4-Chloroindoline
(R)-(-)-2-(2,5-Dihydrophenyl)glycine
2-METHYL-3-HEPTANONE
3,3,3-Trifluoro-1-propanol
1',3',3'-TriMethyl-6-hydroxyspiro(2H-1-benzopyran-2,2'-indoline)
2,5-Diacetoxytoluene
N,N-DiMethyl 3-broMo-5-trifluoroMethylbenzenesulfonaMide
Tributylphosphine oxide, 98%
PotassiuM Binoxalate, Anhydrous, Reagent
1,4-Cyclohexanediol (cis- and trans- Mixture)
3-Chloro-o-xylene
3-Dimethylaminopropylchloride hydrochloride
dodecylsilane
4-Trifluoro Methyl Salicylic Acid
Diethyl adipate
MagnesiuM Perchlorate, Anhydrous
3,5-Difluoro-4-Methoxybenzonitrile, 97%
IndiuM(III) acetate, 99.99% (Metals basis)
ACES, 99%
2,4,6-Tri(9H-carbazol-9-yl)-1,3,5-triazine
4-Fluorophenethyl isocyanate
(S)-N-Boc-1-Naphthylalanine
Pregnenolone
4-BroMo-2-hydroxypyridine
3-BroMo-4-fluorophenylboronic acid
3-(3-Methyl-1,2,4-oxadiazol-5-yl)benzyl bromide 97%
1-Tridecene
6-Bromo-3-oxo-4H-benzo[1,4]oxazine
1-Butyl-4-methylpyridinium Hexafluorophosphate
2,3,5-Trimethylphenol
2-Chloro-6-phenylnicotinonitrile
Butyl formate
4-Ethylbenzoic acid
Silver iodide
4-[(tert-ButoxycarbonylaMino)Methyl]pyridine-2-carboxylic Acid [Reagent for application of the exciton chirality Method]
3-Chloro-5-isopropoxyphenylboronic acid, pinacol ester
Bis(triMethylsilyl)carbodiiMide, 98%
1-(5-BroMo-2,3-dihydro-1H-indol-1-yl)ethan-1-one, 97%
4-AMino-2-chloropyridine
Flavin Adenine Dinucleotide DisodiuM Salt Hydrate
diatrizoic acid sodium salt dihydrate
1-Pyrrolidinecarboxylic acid, 4-hydroxy-2-oxo-, 1,1-dimethylethyl ester
N-Methoxy-N-Methyl-Propionamide
3-bromo-4-methyl-5-nitropyridine
1-(4-BROMOBUTOXY)-2-BROMOBENZENE
DiethylMalonyl dichloride
N-TRIDECYLCYCLOHEXANE
2,5-Bis(triMethylsilyl)thiazole, 96%
DODECAFLUOROSUBERIC ACID
Chloroacetyl-DL-serine
3-Formylphenylboronic acid
2,5-Dimethyl-4-nitroaniline
1-(Hydroxymethyl)-5,5-dimethylhydantoin
Benz[cd]indol-2(1H)-one
Sodium nitrite
Perfluorobutyl iodide
(R)-Styrene oxide
1,4-Diazabicyclo[2.2.2]octane
4'-Decyloxybiphenyl-4-carboxylic Acid
2-BroMo-5-Methylsulfonylnitrobenzene
Pyrrole-2-carboxaldehyde, 99%
IndoMethacin, USP
4-AMinobenzyl Cyanide
Dicloxacillin sodium
N-(tert-Butyl)benzylamine
1-Naphthalenol,4-bromo-
Guaiacol
FN ELISA Kit
BMG/β2-MG ELISA Kit
Human OBSCN ELISA Kit
Human SNX13 ELISA Kit
MSTN ELISA Kit
Rat P-Selectin ELISA Kit
IL-12/70 ELISA Kit
FSH ELISA Kit
AGT ELISA Kit
ADCY3 ELISA Kit
Reinforced Clostridium Medium
YPD Broth
Isoquinoline
GadoliniuM(III) perchlorate hexahydrate, 50% w/w aq. soln., Reagent Grade
5-Chlorouracil
2-Fluoropyridine-4-carboxylic acid, 98%
3-Chloro-o-xylene
4(7)-NITROBENZIMIDAZOLE
Ammonium dichromate
Methyl 2-Fluoroisobutyrate
AC-D-NVA-OH
Oxalyl dihydrazide
Beta-D-Galactose Pentaacetate
2-ForMylthiophene-4-boronic acid, 98%
Methyl carbaMate, 99%
Tetrodotoxin
Methyl Fluoroacetate
4-FLUORO-3-(TRIFLUOROMETHYL)BENZYL BROMIDE
Bis(tetraMethylcyclopentadienyl)chroMiuM(II), 98+%
3,4-DIBENZYLOXYBENZYL CHLORIDE
N-(4-ACETYLPHENYL)MALEIMIDE
1-[4-Chloro-3-(trifluoromethyl)phenyl]thiourea 98%
3-Chloromethyl-5-methylisoxazole
Coconut oil, refined, pure
N-(2-Aminoethyl)-N-ethyl-m-toluidine