L-929[L929]细胞系—小鼠结缔组织L细胞系开学季折扣更多 货号:GD-C16519161
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞系—其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
2-(4-Ethylphenyl)-5-n-propylpyriMidine, 99+%
Celastrol
DIISOPROPYL METHYLPHOSPHONATE
(S)-(+)-2-AMino-1-hexanol
2'-AMINO-3'-HYDROXYACETOPHENONE
Cbz-L-Tyrosine benzyl ester
CIS-1,2-DICHLOROETHYLENE
2-Chloro-5-fluorotoluene
4-(2-Phenylethoxy)benzoic acid
5-Chloro-benzo[b]thiophene
1-Hexacosanol
N,N,N',N'-TETRAMETHYL-1,3-BUTANEDIAMINE
4'-(Chloroacetyl)-acetanilide
Isobutyl hexanoate
5-Ethyluracil
Quinhydrone
DiMethyl trans-1,2-Cyclohexanedicarboxylate
N-VinylforMaMide
Tropine, 98%
SodiuM BisMuthate, Powder, Reagent, ACS
2,6-Dinitroaniline
ethyl 1,2-dichloroethyl ether
calcium bis(4-aminosalicylate)
1,4-Dimethylpyrazole
1-(bromomethyl)-2-(trifluoromethyl)benzene
2-Bromobenzoic acid
Dichloroacetyl chloride
Cedarwood Oil
3,5-DibroMo-4-Methylphenol, 97%
2-BroMo-3'-Methoxyacetophenone, 98%
XMC
Dess-Martin Periodinane
MontMorillonite (AluMinuM Pillared Clay)
Phenazopyridine hydrochloride
3-AMino-5-cyclobutyl-1H-pyrazole
4-AMinobenzylaMine
(+)-2,3-O-Benzylidene-D-threitol, 98%
1-benzylpiperidin-3-amine(SALTDATA: FREE)
2-Chloro-4-(3-methoxyphenyl)pyrimidine
4,4'-Dithiodianiline
10-METHYLPHENOTHIAZINE
2-CHLORO-6-METHYLPHENOL
Trimethyl orthobutyrate
DICHLOROMETHANE-D2
3,4-DibroMothiophene
2-AMino-5-chlorothiazole hydrochloride
Isovalerylshikonin
2-BroMo-6-fluorobenzylaMine
TripalMitin, 99%
Arecoline hydrobromide
4-Fluoro-alpha-Methylstyrene (stabilized with TBC)
2,4-Dichloro-3-nitropyridine
4-Heptyn-2-ol
4-bromo-1,3-dimethyl-1H-pyrazole
(p-aminophenoxy)acetic acid
3,4,5-Trifluorophenylboronic acid
PENTAERYTHRITYL TETRACHLORIDE
(R,R)-(+)-HYDROBENZOIN
Celliton Fast Pink B
2,2-Difluorobutyric acid
4-Amino-2-bromophenol
5-methyl-1,2,4-oxadiazol-3-amine(SALTDATA: FREE)
4'-Methylformanilide
Vinyltriethylsilane(hexafluoroacetylacetonato)silver(I) (99.9%-Ag)
3-METHOXY THIOANISOLE
4-Benzyloxy-3-methylbenzeneboronic acid
2-Bromobutane
6-(1,4-Diazepan-1-yl)nicotinonitrile
1-Benzothiophen-2-ylMethylaMine
N,N-DiMethyl-1,4-cyclohexanediaMine (cis- and trans- Mixture)
1-Cyclopropyl-2,5-diMethyl-1h-pyrrole-3-carbaldehyde
1-Methyl-6,7-diMethoxy-3,4-dihydroisoquinoline, 99+%
Zinc, 30 Mesh, Granular, Reagent, ACS
(+)-Dibenzoyl-D-tartaric Acid
linoleic acid, sodium salt
2-(methylsulfonyl)benzothiazole
L-Glutamic acid-5-methyl ester
Maleimide
Hepc ELISA Kit
TLR9 ELISA Kit
Apo-B100 ELISA Kit
Human AADAC ELISA Kit
IL6 ELISA Kit
α1AT ELISA Kit
IFNα ELISA Kit
Human monocyte chemotactic protein 1/monocyte chemotactic and activating factor,MCP-1/MCAF ELISA Kit
Human von Willebrand Factor,vWF ELISA Kit
TPO ELISA Kit
Psychrophilic Bacterium Count Agar
Mycoplasma Agar Medium
Aluminium isopropoxide
HuMic acid sodiuM salt
5-Chloro-1-(4-piperidinyl)-2-benziMidazolidinone, 98%
2-Fluoro-5-(TrifluoroMethyl)BenzylaMine
ethyl 1,2-dichloroethyl ether
H-D-Asp(OBzl)-OH
N-Acetyl-D-Phenylalanine
Ethyl 3-amino-4-fluorobenzoate
2,3-Difluorobenzoic acid
THYMIDINE 5'-TRIPHOSPHATE SODIUM SALT
2-(TrifluoroMethoxy)BenzylaMine
7-Hydroxy-4-Methyl-8-nitrocouMarin, 98%
3-Hydroxybenzyl alcohol, 98%
β-NicotinaMide adenine dinucleotide phosphate
5-Methoxy-2-nitroaniline
2-AMINO-1-(2-FLUORO-PHENYL)-ETHANOL
3,5,5-TriMethylhexanoyl chloride
(S)-(+)-2-AMino-3-cyclohexyl-1-propanol hydrochloride
Quinacrine Dihydrochloride Hydrate
3-Amino-2-bromo-4-methylpyridine
3-Bromo-5-fluoroanisole
2-(2,4-Dinitrophenylthio)benzothiazole
Potassium (3-fluoro-4-methoxyphenyl)trifluoroborate