HUV-EC-C细胞系—人脐静脉内皮细胞系要多久复苏

HUV-EC-C细胞系—人脐静脉内皮细胞系要多久复苏 货号：GD-C16419178

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息，我们拥有丰富的细胞系培养经验，能提供细胞培养全方位的技术支持，让您实验再无烦扰！公司由在国外工作和学习多年的留学人员创办，由国内ZG科学院上海生物化学与细胞生物学研究所，复旦大学，同济大学等多名工作在线的细胞培养专家，提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念，以世界的技术支撑阵容，提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美，提供了重要的基石；公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品，立足国内，面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位，为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内，在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室，以便更好的服务国内广大医院、大学、制药企业等，你们的每一份关心和支持，都是我们前进的动力！
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，原因比较复杂，常见原因可归纳为：培养基使用错误或培养基品质不佳；血清使用错误或血清的品质不佳；解冻过程错误；冷冻细胞解冻后，加以洗涤细胞和离心；悬浑细胞误认为死细胞；培养温度使用错误；细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来，其离心速率应为多少转速?欲回收动物细胞系—其离心速率一般为300×g(约1OOOrpm),5-10分钟，转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2，其中r为离心机转轴ZX与离心套管底部内壁的距离；rpm为离心机每分钟的转数；RCF(relative centrifugal force)为相对离心力，以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
(2S,3S)-3-(Boc-aMino)-1,2-epoxy-4-phenylbutane, 98%
Azocyclotin
MagnesiuM hydroxide
2,3-Difluorobenzonitrile
ETHYL CHLOROFLUOROACETATE
ETHYL 4-CHLORO-5,7-DIMETHYLQUINOLINE-3-CARBOXYLATE
HENEICOSANOIC ACID
1-[2-Chloro-4-(4-chlorophenoxy)phenyl]ethan-1-one , 95%
5-Chloro-2-methoxybenzonitrile, 97%
4-Bromo-7-methylisatin
Sodium Abietate
3',4'-DIMETHYLACETANILIDE
Deslanoside
2-Bromoanthraquinone
Benzil
Tri-p-tolyl phosphate
Diethyl cis-4-Cyclohexene-1,2-dicarboxylate
2,6-DiMethylaniline Hydrochloride
Undecanoic acid, 99%
SodiuM Glucoheptonate
Methyl 4-AMino-3-Methylbenzoate
1,2-Propylene diacetate
3,4,6-Trichloropyridazine
Benzylhydrazine monohydrochloride
2-Fluoro-4-methylphenol
CYCLOPROPANEMETHYLAMINE HYDROCHLORIDE
ACETIC ACID
5-BroMo-1H-Indole-3-Carboxylic Acid
2-Thiopheneacetic acid, 98%
2-(Chloroethyl)benzene, 99%
4-Dodecylbenzenesulfonic acid
Saikosaponin C
N-CAPRIC ACID ISOAMYL ESTER
PERFLUORO-2,5,8-TRIMETHYL-3,6,9-TRIOXADODECANOYL FLUORIDE
7-(TRIFLUOROMETHYL)QUINOLINE
2-BroMothiazole-4-carboxylic acid
1-(4-methyl-2-thienyl)ethanone(SALTDATA: FREE)
3-(4-Methylpiperazin-1-yl)benzoic acid
(2,3-Dihydro-benzo[1,4]dioxin-6-yl)-acetic acid
2,4,6-Trimethylphenyl isothiocyanate
Bromocresol Purple
N-(2-Hydroxyethyl)acetamide
2-NONENOIC ACID
4-Methoxybenzoyl chloride
2,3-Dihydro-1-benzofuran-5-carbonyl chloride
Tauroursodeoxycholic Acid Dihydrate
Tetrakis(ethylthio)tetrathiafulvalene [Organic Electronic Material]
2-AMino-2-Methyl-1-propanol, 99%
4-(PhenoxyMethyl)benzoic acid, 97%
S-[N-(3-PHENYLPROPYL)(THIOCARBAMOYL)]-L-CYSTEINE
1-Butyl-4-MethylpyridiniuM Chloride
Esmolol
2-Methoxy-5-nitro-3-picoline
1-Methyl-1H-imidazole-4-carboxylic acid
Methyl 5-methylsalicylate
4-Pyridinecarboxaldehyde
Cholesteryl palMitate
Z-Lys(Z)-OSu
ChlorodiMethylphenylsilane
β-Galactosidase [ 400 units/mg]
ethyl (4-formyl-2-methoxyphenoxy)acetate(SALTDATA: FREE)
Cinnamoyl chloride, predominantly trans, 98%
3-Chloro-2-(4-methylpiperazin-1-yl)pyridine
Sclareol
TRIBROMONEOPENTYL METHACRYLATE
2-(2-(3-(2-(7-Chloro-2-quinolinyl)-ethenylphenyl)-3-hydroxypropyl)phenyl)-2-propanol
2-phenyl-2-iMidazoline
Victoria Blue B
1-Benzyl-3-(MethylaMino)pyrrolidine
2-(t-ButylsulfaMoyl)-5-Methylphenylboronic acid
AMberlite? IR120, Na forM, ion-exchange resin
2,4-Difluorobenzaldehyde, 98%
2-(TriMethylsilyl)ethyl 3-Nitro-1H-1,2,4-triazole-1-carboxylate
2-Phenoxypropionic Acid
(E)-o-Hydroxycinnamic acid
Methyl 4-butylacetamino-3-methylbenzoate
2-(3-Fluorophenyl)-2-methylpropanoic acid
Butanoic acid,2-amino-3-oxo-,ethyl ester
GSK-3 ELISA Kit
TIEG1 ELISA Kit
Pre-APO-A1 ELISA Kit
Human ARSF ELISA Kit
IL-2 ELISA Kit
α2M ELISA Kit
Chicken BMP-4 (Bone Morphogenetic Protein 4) ELISA Kit
Human MCP-3 (Monocyte Chemotactic Protein 3) ELISA Kit
Human VASH1 ELISA Kit
GYPE ELISA Kit
Acid Broth
Nutrient Agar with TTC
FORMATE ION CHROMATOGRAPHY STANDARD
PotassiuM hydrogen fluoride, 98%
5alpha-Androstane-3b,17b-diol
2-Fluoro-5-iodobenzonitrile, 97%
1,2-Propylene diacetate
Ethanone, 1-(3-hydroxy-2-pyridinyl)-
4-PHENYLPYRAZOLE
3-fluorobenzyl chloride

3-TERT-BUTYL-1-METHYL-2-PYRAZOLIN-5-ONE
Titanium tetraisopropanolate
7-Fluorooxindole
SodiuM n-heptafluorobutyrate, 98%
Bis(tert-butylcyclopentadienyl)diMethylhafniuM(IV), 98+%
2-AMinopyridine
4-Methylpyridine-2-carboxaldehyde
2'',4'',6''-TRIFLUOROPROPIOPHENONE
N-Methoxy-N-MethylbenzaMide
3-AMino-L-tyrosine dihydrochloride Monohydrate
(S)-3,3-Diphenyl-1-Methylpyrrolidino[1,2-c]-1,3,2-oxazaborole
3-(Methoxycarbonyl)-5-nitrobenzeneboronic acid
4-(4-Propylphenyl)benzoic Acid
(S)-(-)-2,2'-Bis(diphenylphosphino)-1,1'-binaphthyl, 99+%
Potassium niobate (99.999%-Nb) PURATREM