C-33 A细胞系—人子宫颈癌细胞系要多久复苏 货号:GD-C16419102
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞系—其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
Triethylborane, 1M soln. in THF
5-Methyl-2-hexanone
Tetrakis(acetonitrile)copper(I) tetrafluoroborate
2-BroMo-4,6-difluorophenol, 98+%
ETHYL N-ETHYLCARBAMATE
Maltitol
(-)-PERILLALDEHYDE
1-Tetrahydro-2H-pyran-3-ylmethanamine
6-Chloronicotinonitrile
2-(Hydroxymethyl)-15-crown 5-Ether
3-Bromobenzylamine, 95%
3-Hydroxy-4-nitrobenzaldehyde
Isopropylphenyl phosphate
STEARYL ACETATE
2-BroMobenzyl BroMide
Indole-5-carboxylic acid
1,4-DibroMooctafluorobutane
Methyl 2-benzyloxy-4-broMobenzoate
Tri-n-propylsilane, 99%
Resveratrol
4-(TrifluoroMethyl)benzylaMine
tricaprin
Rhodanine-N-acetic acid
1H-pyrrole-2,3,5-tricarboxylic acid
7-Fluoroindole
4-Bromo-3-fluorobenzenesulfonamide
2,3-Dichlorobenzaldehyde
3'-Chloro-4'-fluoroacetophenone, 97%
SeleniuM dichloride oxide, 99% (Metals basis)
2-Fluorobenzonitrile
2-Furanylboronic acid MIDA ester
Phthalic anhydride
Bis(diphenylphosphino)Methane
1-PHENYL-1-NONYNE
2,5-Difluorobenzotrifluoride
2,6-Dibromo-4-isopropylaniline
3-(4,4,5,5-Tetramethyl-1,3,2-dioxaborolan-2-yl)acetanilide ,98%
4-Methoxysalicylamide
L-Glutamic acid 5-methyl ester
METHYL 4-CHLORO-2-NITROBENZOATE
Cefpirome sulfate
DL-3-Methylvaleric acid
1-Chloro-2-iodobenzene (stabilized with copper chip)
Cholesterol Lauryl Carbonate
2-(MethylcarbaMoyl)phenylboronic acid
EthylenediaMinetetraacetic acid, disodiuM salt, 99+%, for Molecular biology, DNAse, RNAse and Protease free
2-Chloro-6-fluorophenylboronic acid, 97%
3-BroMo-o-xylene
Ethyl 3-AMinobenzoate
2-Aminopyrimidine-4-carboxylic acid
2-Methoxybiphenyl
(1S,3R)-N-BOC-1-Aminocyclopentane-3-carboxylic acid
1,1'-Biphenyl, 4-bromo-4'-iodo-
Amino tris(methylene phosphonic acid)
3-Chloro-2-fluorothioanisole
4-Benzylaniline, HCl
RuCl2[(S)-(DM-BINAP)][(S)-DAIPEN]
1-(2-Cyanoethyl)-3-(trifluoromethyl)-pyrid-2-(1H)-one
3,4-Dimethylaniline, 99.5%
Allylacetoacetate, 98%
4-Aminothiomorpholine 1,1-Dioxide
Methyl-4-nitro-1-methyl pyrrole-2-carboxylate
2-Bromo-1-[4-(methylsulfonyl)phenyl]-1-ethanone
Tetramethoxymethane
2,2-Dimethylpentane
SodiuM hyaluronate
1-AcetaMidoadaMantane
BisMuth SodiuM EthylenediaMinetetraacetate
2-(BenzylaMino)nicotinonitrile
1-BroMooctadecane, 96%
2,4,5-TRICHLOROANISOLE
Bis(4-chlorobutyl) Ether
3-Methyl-2-pyridinol
Vanadium atomic absorption standard solution
2,7-Naphthalenedisulfonic acid disodium salt
methyl O-acetylsalicylate
VD3 ELISA Kit
Mouse α1-microglobulin,α1-MG ELISA Kit
Human PSEN1 ELISA Kit
Human LTA ELISA Kit
IC ELISA Kit
TLL1 ELISA Kit
Chicken C3 (Complement Component 3) ELISA Kit
Human FMR1 ELISA Kit
Human VCAM-1 ELISA Kit
VEGFR 3 ELISA Kit
N6 Medium without Agar
Glfu Anaerobic Medium Agar
AluMinuM cobalt isopropoxide, 99.9% (Metals basis), 10% w/v in isopropanol
Glycine Methyl ester hydrochloride, 99%
5-Chloro-2-Methoxypyridine, 98%
2-FluorobenzylaMine, 97%
tricaprin
Reactive Yellow 39
calcitonin
5-fluoroquinoline
DL-2-Amino-1-butanol
Cupric Sulfate
2-Benzyloxypyridine-3-boronic acid pinacol ester, 95%
3',4'-Dichloroacetophenone, 98+%
Ethyl (phenylthio)acetate, 97%
HolMiuM chloride hexahydrate
N-HydroxyMaleiMide
(R,R)-(+)-N,N′-Bis(α-Methylbenzyl)sulfaMide
FUMARAMIDE
BenzyldiMethylhexadecylaMMoniuM chloride
Ethyl 3-Methylthiopropionate
Perfluoroglutaryl chloride 98%
3,5-Dichloro-4-hydroxybenzaldehyde
Octadecyl Isocyanate
Cobalt(II) carbonate hydrate (99%-Co) (Co min. 45.5%)